Search results

...sheets for different SUIT protocols are incorporated in DatLab (DL7.1): [[Protocols in DatLab]] Library of SUIT protocols [[MitoPedia:_SUIT#SUIT_protocols|MitoPedia: SUIT protocols]]

...le=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Laboratory protocol: isolation of rat heart mitochondria. |abstract=Sumbalova Z, Fontana M, Krumschnabel G (2016) Laboratory protocol: isolation of rat heart mitochondria. Mitochondr Physiol Network 2

...|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: Isolation of rat liver mitochondria. |abstract='''Lassnig B, Gnaiger E. Laboratory protocol: Isolation of rat liver mitochondria. Mitochondr Physiol Network 0

...ls.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] |abstract=Sumbalova Z, Fontana M, Krumschnabel G (2016) Laboratory protocol: Isolation of rat brain mitochondria. Mitochondr Physiol Network 2

::::# [[MitoFit protocols]] ::::## [[Harmonization protocols]] (Begley, Ioannidis 2015)

...|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory Protocol: isolation of rat liver mitochondria.

...|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: isolation of beef heart mitochondria.

|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]]Laboratory protocol: isolation of mouse heart mitochondria. |abstract=Komlodi T, Cardoso LHD, Gnaiger E (2021) Laboratory protocol: Isolation of mouse heart mitochondria. Mitochondr Physiol Network

:::: In particular, we are interested in the standardization of protocols to separate blood cells for the respirometry analysis. We do have a large b == Visiting scientist in the Oroboros O2k-Laboratory ==

...'''Vill AT''', 2019 Feb 21. Third retreat of the Daniel Swarovski Research Laboratory (DSL). |abstract= Third retreat of the Daniel Swarovski Research Laboratory (DSL), Innsbruck, Austria

...|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: Complex I (NADH:Ubiquinone Oxidoreductase, EC 1.6.5.3). Mitochond |abstract='''Kuznetsov AV, Gnaiger E. Laboratory protocol: Complex I (NADH:Ubiquinone Oxidoreductase, EC 1.6.5.3). Mitochond

...|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. |abstract=Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: [[Citrate synthase]] a mitochondrial marker enzyme. Mitochondr Ph

...ger.com/biomed/pharmacology+%26+toxicology/book/978-1-4939-2287-1 Springer Protocols] ...sary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

[[File:Lab in action.jpg|right|500px|Oroboros Laboratory]] <!--[[File:OROBOROS MitoFit-Lab.jpg|left|240px |Oroboros O2k-Laboratory]]-->

...n published protocols, it is frequently assumed that the standards of each laboratory are applied as to the quality of purified water. Purification is not only t

...respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the ...k-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.

...[http://link.springer.com/book/10.1007/978-1-4939-2257-4#page=334 Springer Protocols] ...sary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

...tochondrial function assay: monitoring preparative steps and comparison of protocols. In our laboratory we are mainly focused on PBMCs blood cells. One of the main issues of worki

Laboratory Manager * Laboratory infrastructure

...nk=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols contents]]Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. |abstract='''Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr Physio

...cols published over the years, the aim of my STSM consists in comparing my protocols with those used in the host lab in order to harmonize our procedures and, a ...nity; in particular our aim is the interlaboratory comparisons of specific protocols, a critical issue in results interpretation.

...BOROS MitoFit-Lab.jpg|right|210px|link=Oroboros_Laboratories |Oroboros O2k-Laboratory]] ...control in high-resolution respirometry, aimed at standardization of SUIT protocols and fostering proficiency to generate experimental data of assured high qua

|institution=Laboratory of Cellular Metabolism, ::::I am employed as an associate professor at the Laboratory of Cellular Metabolism of the Nencki Institute of Experimental Biology. Sin

...eft|90px|Carolina Doerrier]] Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers.

:::: <big><big><big>'''Laboratory standards for high-resolution respirometry: towards a QCS'''</big></big></b ...overcome, providing a rationale for establishing a set of basic reference protocols.

|institution=Laboratory of Pharmaceutical Pharmacology, ...ise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabo

...h-2k (Oroboros Instruments, Innsbruck, Austria). We will optimize the SUIT protocols with defined substrate combinations, uncoupler and inhibitor titrations, wh == Visiting scientist in the Oroboros O2k-Laboratory ==

...ise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabo == Visiting scientist in the Oroboros O2k-Laboratory ==

:* Harmonization of respirometric protocols comparing isolated mitochondria and permeabilized muscle fibers ...the Action. The STSM will aid in the standardization and harmonization of protocols that increase the value and reduce the noise in mitochondrial research. The

...t I will get, it could lead to the introduction of learned methods in home laboratory. ::::# The 14th to 23th January 2020: training in laboratory techniques for (1) hPBMCs preparation: blood sampling, transport, storage a

...le in the progression of non-alcoholic steatohepatitis, therefore improved protocols are required to evaluate mitochondrial fitness in the course of disease. Et :::: 2. The goal of this secondment is to provide standardized protocols to study FAO and to deepen our knowledge about the effect of etomoxir on F-

|institution=[[File:Vendelin Marko.jpg|right|150px|Vendelin Marko]] Laboratory of Systems Biology, ...spirometer. This alternative hardware would allow us to verify whether the protocols work on them as well.

...f MitoEAGLE. During the 8-day STSM mission I could acquire new measurement protocols which could be applied in future projects. These are the planned program of ...SM project could be a basis of a flourishing cooperation with the Oroboros laboratory in Innsbruck in the near future.

...mphasis on internal quality control and possible adaptation of measurement protocols to samples from various tissues. Furthermore, we are going to use tools of ...rm having up-to-date instrumental background, well established measurement protocols and top level proficiency.

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: ...roficiency_test| MitoFit Proficiency test]] for inter-individual and inter-laboratory reproducibility quality control.

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: ...roficiency_test| MitoFit Proficiency test]] for inter-individual and inter-laboratory reproducibility quality control.

::::» [[Oroboros Laboratory: visiting scientists]] ...s may join us for a defined project in the Oroboros Mitochondrial Research Laboratory. The scholarship will provide an in-depth introduction or extension of pre

...preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]]. :::: '''»''' [[Oroboros Laboratory|Oroboros O2k-Laboratory]] - open for innovation and cooperation.

...lls and biopsied skeletal muscle tissue using complementary approaches and protocols. In addition to standard clinical measures, analyses of physical ability an == Visiting scientist in the Oroboros O2k-Laboratory ==

...mpared and strategies defined for improvement of quality control. An inter-laboratory ring test will be established as a world-wide innovation in the field of mi :::: D. Swarovski Research Laboratory

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: ...roficiency_test| MitoFit Proficiency test]] for inter-individual and inter-laboratory reproducibility quality control.

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: ...roficiency_test| MitoFit Proficiency test]] for inter-individual and inter-laboratory reproducibility quality control.

...stions. Furthermore, this workshop covers interpretation of data from SUIT protocols. ::::» Oroboros O2k-Laboratory and ''MiPArt''

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: ...roficiency_test| MitoFit Proficiency test]] for inter-individual and inter-laboratory reproducibility quality control.

...a Sumbalova]], [[Krumschnabel G |Gerhard Krumschnabel]]: Towards reference protocols. * Standard protocols for blood cell separation, cell type characterization (purity, composition)

...ver 'ethanol abs.' is mentioned without further specification in published protocols, it refers to ≥ 99 % ethanol a.r. (analytical reagent grade).

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows to: ...roficiency_test| MitoFit Proficiency test]] for inter-individual and inter-laboratory reproducibility as quality control.

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: ...roficiency_test| MitoFit Proficiency test]] for inter-individual and inter-laboratory reproducibility quality control.

...ata and finalize the realization of a joint publication aimed to harmonize protocols and procedures for handling and analyzing mitochondria of PBMCs and PLTs. == Visiting scientist in the Oroboros O2k-Laboratory ==

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: ...roficiency_test| MitoFit Proficiency test]] for inter-individual and inter-laboratory reproducibility quality control.

...lls and biopsied skeletal muscle tissue using complementary approaches and protocols. In addition to standard clinical measures, analyses of physical ability an == Visiting scientist in the Oroboros O2k-Laboratory ==

...d inhibition of ETC components. Under such conditions mSLP is addressed by protocols relying on membrane potential measurements using safranine O, also monitore == Visiting scientist in the Oroboros O2k-Laboratory ==

...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown during the training. The [[Gnaiger 2020 BEC MitoPathways| ...xtremely helpful and provide much guide and information regarding the O2k, protocols and bioenergetics.

...n in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establi ...onomical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability.'' -

== Specific SUIT protocols == ...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows:

...lated mitochondria and permeabilized tissue to standardized and harmonized protocols and applications with tissue slices. Before starting this experimental plan == Visiting scientist in the Oroboros O2k-Laboratory ==

...endence only on limited amount of canonical substrates. Furthermore, these protocols can be very helpful for identifying substrates of other mitochondrial metab ...Slc25a34-KD) from my home laboratory and grow and differentiate them in a laboratory in the host institute. As an interesting extension (during the STSM or afte

== Specific SUIT protocols == ...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows:

...Action MITOEAGLE. Moreover, I will continue the studies on methodology and protocols for mitochondrial analyzes in PBMCs using new techniques I will learn durin == Visiting scientist in the Oroboros O2k-Laboratory ==

...physiology. In conclusion, an optimal design and comprehension of the SUIT protocols is crucial for a proper evaluation of mitochondrial function in healthy and ...Dept Visceral, Transplant and Thoracic Surgery, Daniel Swarovski Research Laboratory, Medical University Innsbruck, Austria

...and substrate control states [1] were established in 6 different [[SUIT]] protocols. In calculation of ΔΨ, mitochondrial protein was normalized to citrate sy |keywords=ΔΨ, Mitochondrial preparations, [[SUIT]] protocols

...ide optimisation of the methods for new users, and provide full laboratory protocols in Supplementary materials.

...nimals was evaluated according to the neurological deficit scale for small laboratory animals and Garcia’s scale. Mnestic and cognitive abilities were also tes Protocols of acute and chronic prenatal hypoxia modeling for mice have been designed.

...opose potential solutions for these problems, including '''improvements in protocols and documentation''', consideration of evidence from studies in progress, ' ::::* Make publicly available the full protocols, analysis plans or sequence of analytical choices, and raw data for all des

...ture of biomedical and patient specimens with respect to experimental SUIT protocols to be applied.

...velopments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols in HRR using the O2k-Fluorescence LED2-Module for simultaneous measurement ...rondheim_Rognmo_O]] uses three O2k-instruments as a [[Power-O2k| Power-O2k Laboratory]].

...ototype test scenario and expanding the range of application-specific SUIT protocols in the MitoFit-data base. O2k-Core applications are extended by application

...sions and new, user-friendly features on [[Run DL-Protocol/Set O2 limit|DL-protocols]], [[Export DL-Protocol User (*.DLPU)|DL-Protocol User]], quality control, ...ds= DatLab, [[MiPNet22.16 DatLab 7 Innovations: DL-Protocols|Innovation DL-Protocols]]

...pects of flow cytometry, pointing to the key elements of the commonly used protocols for determining of multiple parameters of blood platelet (patho)physiology

::::* [[Doerrier 2018 MiP2018a |Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers]] ::::* [[Doerrier 2018 MiP2018c |Inter-laboratory study on human permeabilized myofibres: effects of fibre preparation, respi

...E, Garcia-Roves PM (2020) '''Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers.''' - Updated: 2020-03-08 ...E is preparing a manuscript 'Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers'. The group is working on it with Open

The experiments were carried out following SUIT protocols, with minor modifications [2]. We investigated Complex I and II and a combi ...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

|Demo: Instrumental quality control 1. Introduction to SUIT protocols RP1&RP2 |SUIT protocols

...nings concerning the quality control in high-resolution respirometry, SUIT protocols, experimental designs and DatLab analysis. == Visiting scientist in the Oroboros O2k-Laboratory ==

...s, living cells). Optimized substrate-uncoupler-inhibitor titration (SUIT) protocols offer a powerful tool for comprehensive mitochondrial physiology analysis b #proficiency tests with reference samples for inter-laboratory harmonization[4,5]; and

...to Innsbruck 22 January – 2 February “ training days in Oroboros MitoFit laboratory 3 February“ departure from Innsbruck During STSM my objectives will be: == Visiting scientist in the Oroboros O2k-Laboratory ==

...offering practical guidance and insights from experiments performed in our laboratory, as well as examples of the applicability of such a protocol. <br>

...work as research assistant in the Latvian Institute of Organic Synthesis, Laboratory of Pharmaceutical Pharmacology (under supervision of Prof., Dr. Pharm. Maij ...cts on mitochondrial and extra-mitochondrial glucose metabolism in another laboratory setting using myocyte cell lines. In addition, results obtained in the Musc

...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days. ::::» Oroboros O2k-Laboratory

...019 MitoFit Preprint Arch EA|Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers]]'''''</big>. :::: DSL-Oroboros Research Laboratory

...inclusion of large animal models is necessary to develop safe preclinical protocols. Therefore, we tested the hypothesis that addition of MitoQ to cold storage

...ation, detailed descriptions of [http://www.oroboros.at/?O2k-protocols O2k-Protocols] on our website, and direct communication with our customers and O2k-users ...ntribute to a more general understanding of the rationale for standardized protocols, while the Bioblast wiki website (launched in 2010) may help to develop a m

[[Image:O2k-Protocols.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Procedures |O2k-Pr ...ttp://wiki.oroboros.at/index.php/The_world_as_a_laboratory |The world as a laboratory]]

...provement over time. Ongoing quality assessment helps laboratories improve protocols and performance on a real-time basis to ensure optimal cryopreservation of

...by using different types of Substrate-Uncoupler-Inhibitor-Titration (SUIT) protocols (SUIT1 and SUIT2) where we evaluated the possible deviations between differ ...unlimited titrations and, therefore, the possibility to apply various SUIT protocols designed specifically for a certain project.

...y final '''best'''. QA in general may set impractically high demands on a laboratory, hence the '''required level of QA depends on the specific application'''. ...lved), the individual scientist (all of us, all of our collaborators), the laboratory, mt-respiratory physiology in terms of scientific reputation (including som

...er E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. https://doi.org/10.26124/mitofit:ea19 ...tofitea19.MiPSchool.0009.pdf Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers]

:::: '''Standard operating procedures and user requirement document: Protocols, terminology, documentation''' ::::» [[WG1 MitoEAGLE protocols, terminology, documentation |WG1 Action]]

Applied Surgery and Metabolism Laboratory ...OROS is Erich's groups outstanding efforts to provide detailed methods and protocols for both the machines and experiments. ...'' '''[[Hickey Anthony JR|Anthony

...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days. ::::» Oroboros O2k-Laboratory

...|left|90px|Carolina Anneliese Doerrier]] Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers. ...rted data on mt-respiration from the scientific literature and inter/intra-laboratory comparisons are very difficult. In permeabilized muscle fibers (pfi) one of

...peroxide production. Many optimized [[SUIT protocols]] are available as DL-Protocols. The [[SUITbrowser]] helps you find the best SUIT protocol for your specifi ...t, the lecture by Erich Gnaiger on Monday, 11:15-12:00, on 'The world as a laboratory: Exploring mitochondrial fitness in human populations in extreme environmen

...tion protocols not only for PBMC but including PLT, applying respirometric protocols specifically developed for diagnostic monitoring. ...amage of PLT by cryopreservation. Further optimization of cryopreservation protocols for blood cells provide new tools for storage and effective use of these ce

(2) Harmonization of experimental procedures (protocols) is a basic requirement to increase the robustness of information and knowl ...s to research targeted at hypothesis testing. Experimental optimization of protocols including negative results should be made publicly available at an early ti

...atLab 7 Innovations |DatLab 7.3]] - see [[Run DL-Protocol/Set O2 limit |DL-Protocols]] ::::# Instrumental quality control with DL-Protocols.

...ring the STSM I will participate in the experiments carried on in the host laboratory using the above mentioned techniques and then I will perform them myself.

...topics in Mitochondrial Physiology' extend the discussions on respiratory protocols. ...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory

[[Image:The world as a laboratory.jpg|right|180px|link=http://wiki.oroboros.at/index.php/The_world_as_a_labor ...Williamson''' ([[US OK Stillwater Davis MS|Comparative Exercise Physiology Laboratory, Oklahoma State University]]) with [http://www.buserdog.com '''Martin Buser

# Protocols for high-resolution respirometry: focus on combined measurement of respirat ...we offer pre-expedition training to international partners in our research laboratory, for scientists and students to gain competence for the challenges met when

...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory *'Specific protocols and procedures for cells and isolated mito.'

...donors: 4 to 5 • Respiration media: MiR05 •Cell type: freshly isolated PLT Protocols (respirometry/Fluorometry): • AmR_SUIT 9_pce: 1S;2D;3P;4Ama • SUIT 7: c == Visiting scientist in the Oroboros O2k-Laboratory ==

[[Image:The world as a laboratory.jpg|right|180px|link=http://wiki.oroboros.at/index.php/The_world_as_a_labor ...Dr.a Isabel Moreno (right) belongs to the physics staff of the Chacaltaya laboratory (Jul/Aug 2012).]]

...days) prior to being saponin permeabilized for HRR using a variety of SUIT protocols on an Oroboros Oxygraph-2k. 1 - Mitochondrial Physiology Laboratory, Department of Health and Exercise Science, Colorado State University, Fort

...Diagnosis of Mitochondrial Defects and Tissue Characterization''' - MiPNet Protocols for High-Resolution Respirometry ...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory (AT)

...rent methods in healthy human volunteers. In addition, we are applying the protocols to a porcine trauma brain injury model, to analyze mitochondrial (dys-)func ...otocols to a porcine TBI model, which we are currently establishing in our laboratory. Our preliminary data suggests that the techniques, established in human PB

...ific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days. ::::» Oroboros O2k-Laboratory

Laboratory of Behavioral Genetics :* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...iable data in commonly used substrate-uncoupler-inhibitor-titration (SUIT) protocols and cyanide titration is needed to obtain precise data for COX excess capac ...xygen sensor were performed with a software package developed for internal laboratory use. In the second part of the study, COX inhibition was tested with sequen

...in many laboratories [1]. Beyond the instrumental level, a standardized mt-laboratory quality management system (QMSmtf [2]) is required for establishing a globa ...ph-2k applying standard substrate-uncoupler-inhibitor-titration ([[SUIT]]) protocols for permeabilized cells in [[MiR05]]. For HRR with intact cells, cyropreser

...with the isolated mitochondria using a conventional oxygraph and standard protocols (performed by Mariana Rosca, M.D. [1]), while for the permeabilized fibers, 2-Daniel Swarovski Research Laboratory, Dept General Transplant Surgery, Med Univ Innsbruck, Austria

...epilept(ogen)ic medication. Therefore, we are in the process of developing protocols to analyze different mitochondrial parameters using the Oxygraph-2K (Orobor ...rtment of Visceral, Transplant and Thoracic Surgery, D. Swarovski Research Laboratory, Innsbruck, Austria

|title=[[Image:DoerrierC.JPG|left|90px|Carolina Doerrier]] Inter-laboratory study on human permeabilized myofibers: effects of fiber preparation, respi ...ented during the MitoEAGLE meeting and next steps will be discussed. Inter-laboratory studies, ring tests, more robust experimental design, and care in statistic

...[3]; 2) a blinded international study measuring simultaneously in the same laboratory respiration of permeabilized human skeletal fibers by high-resolution respi ...er E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. MitoFit Preprint Arch. doi:10.26124/m

...dedicated MitoEAGLE knowledge management network developing harmonization protocols towards generating a rigorously monitored data repository on mitochondrial ...Dept Visceral, Transplant and Thoracic Surgery, Daniel Swarovski Research Laboratory, Medical University Innsbruck, Austria

...y''. Links will be made available to relevant websites, including selected protocols for practical approaches to MiP. In due time, a hard copy will be produced :::<small>Daniel Swarovski Research Laboratory, Department of General and Transplant Surgery, Medical University Innsbruck

...ry OXPHOS control: multiple substrate-uncoupler-inhibitor titrations (SUIT protocols) and enzymatic thresholds. ...ros Instrumetns and Medical University of Innsbruck, D. Swarovski Research Laboratory

:::: National Research Laboratory for Mitochondrial Signaling ::::::* SUIT-Protocols » [[Run DL-Protocol/Set O2 limit]]

|DatLab 7, DL-Protocols. ...nce methods for evaluation of mt-membrane potential. Examples in different protocols.

..., as it offers a large collection of relevant preprints, publications, and protocols, which are always up-to-date and therefore create a platform where recent k ...l in the process of final manuscript preparation, such as harmonization of protocols, which not only help to create clear guidelines for other researchers but a

...om C57BL/6 mice. Respiration media (37 °C) were optimized for the specific protocols. High-resolution respirometry (HRR) was applied with the O2k-Fluorometer (O ...niv, Bratislava, Slovakia; 3-Dept Medical Biochem, Semmelweis Univ, MTA-SE Laboratory Neurobiochem, Budapest, Hungary; 4-D.Swarovski Research Lab, Dept Visceral,

6th floor conference room / nine bench laboratory module for the experimental program <big>'''SUIT protocols for high-resolution respirometry'''</big>

...types of oxygraphs make not such valuable a scientific mission in another laboratory. The applicant in the past had a post-doc who worked with her and was train ...a new technique that will allow her to broaden her field of research. The protocols followed will include: testing of complex I activity (by glutamate plus mal

...of titration volumes and automatic control of complex titration-injection protocols. The TIP2k was specially developed for the O2k. The main modes of operation ...g the solution may be necessary. Small particles can also be introduced by laboratory activities (e.g., from paper towels or from powdered gloves). Only use powd

::: [[MitoEAGLE protocols, terminology, documentation |WG1: MitoEAGLE protocols, terminology, documentation]] ::::* [[Doerrier 2018 MiP2018a |Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers]]

...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory :::: [[Eigentler Andrea]], D. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical Universit

...mitochondrial capacities and create a Library of protocols: reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis ...k-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks for mitochon

...Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and is :::# Daniel Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical Universit

...due to tissue heterogeneity, which restricts comparability when different protocols are applied in parallel in different O2k-chambers – with tissue homogenat ...rtment of Visceral, Transplant and Thoracic Surgery, D. Swarovski Research Laboratory, Innsbruck, Austria; Email: [email protected]

...sion injury [5-8]. These studies established a first level of bioenergetic protocols for biomedical applications of high-resolution respirometry [9-12]. With co ...oration between the Medical University of Innsbruck (D. Swarovski Research Laboratory), Oroboros Instruments and WGT-Elektronik was recognized by granting the Cl

:* Previous laboratory: [[ES Granada Acuna-Castroviejo D]], [[AT Innsbruck Oroboros]]. :* [[Library of protocols]]

[[File:SUIT-nomenclature.jpg|right|400px|link= MitoPedia: SUIT|SUIT protocols]] === A1: Diagnostic SUIT protocols of mitochondrial fitness ===

...Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and is ...ife, Organ Regeneration Center of Excellence and Daniel Swarovski Research Laboratory, Dept Visceral, Transplant and Thoracic Surgery, Medical Univ Innsbruck, In

[[Image:The world as a laboratory.jpg|right|180px|link=http://wiki.oroboros.at/index.php/The_world_as_a_labor ...project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]], D. Swarovski Research Laboratory (Medical University of Innsbruck), and Oroboros Instruments.

...nnovation and [[Gentle Science]], the [[Oroboros Laboratories|Oroboros O2k-Laboratory]] welcomes visiting researchers from all over the world.'''</big> ...OBOROS MitoFit-Lab.jpg|left|240px|link=Oroboros Laboratories |Oroboros O2k-Laboratory]]

=== Lead partner: D. Swarovski Research Laboratory (DSL), Medical University of Innsbruck (MUI) === ...physiology and pathology, for development and application of experimental protocols for functional mitochondrial diagnosis.

...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory ...prehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control. Mitochondrial pathways and coupling'''

...igh oxygen regime can be done using dithionite titrations following the DL-Protocols: Instrumental high O2 background_manual injections or Instrumental high O2 ...dium. All Instrumental DL-Protocols available can be found in C:\DatLab\DL-Protocols\Instrumental\ (default directory after installation of DatLab).

...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory ...ments in the New Year, and I will keep you posted regarding our fatty acid protocols. Hopefully we can create a 'fatty acid substrate' protocol that will become

File:IOC115 (5).jpg|Pedro explaining the steps in the SUIT protocols ...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory

:::: Erich Gnaiger (Medical University of Innsbruck, D. Swarovski Research Laboratory) and his O2k-Team (Oroboros Instruments, Innsbruck) in collaboration with P :::: Medical University of Innsbruck, D. Swarovski Research Laboratory

...tochondrial physiology and pathology, and for a variety of O2k-MultiSensor protocols applied for functional diagnosis of mitochondrial performance. The fast and 2 -Daniel Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical Universit

...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory :::: [[Eigentler Andrea]], D. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical Universit

::::* Integration of the new protocols in the DatLab software ...nd correction, experimental SUIT (substrate-uncoupler-inhibitor-titration) protocols and data analysis.

...lism with subsequent increased use of glucose. As a consequence, human and laboratory animals suffer from an acute hypoglycaemic syndrome due to blockade of gluc Although these protocols do not reveal the MCPA action site(s), these respirometric studies may serv

...Skłodowska-Curie]] secondment at the [[Oroboros Laboratories|Oroboros O2k-Laboratory]]: [[Revenco_2019_Abstract_IOC141|Analysis of mitochondrial respiration in ...Skłodowska-Curie]] secondment at the [[Oroboros Laboratories|Oroboros O2k-Laboratory]]: [[Revenco_2019_Abstract_IOC141|Analysis of mitochondrial respiration in

...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory |'''[[ES Santiago De Compostela Mendez-Alvarez E]]:''' Laboratory of Neuroanatomy and Experimental Neurology, Department of Morphological Sci

::::» [[Oroboros Laboratory]] ...ium will collaborate on the implementation of refined and novel diagnostic protocols as a milestone towards an internationally recognized center of excellence,

::: [[MitoEAGLE protocols, terminology, documentation |WG1: MitoEAGLE protocols, terminology, documentation]] ...19 MitoFit Preprint Arch EA| Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers]]

The project [[K-Regio MitoFit |MitoFit]] aims at developing novel laboratory standards and diagnostic monitoring of a mitochondrial fitness score. With ...working with "mitochondria people", getting to know the Oxygraph, methods, protocols. I am well certain that soon I will be also a user of the Oroboros instrume

:::: D. Swarovski Research Laboratory :::: 2017 - Ao. Univ.-Prof. i.R.; D. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical Universit

...lt the Information for Authors of the particular journal and should report laboratory information in both local and International System of Units (SI). ...thor contributions and acknowledgements. Taken together, '''the 'Vancouver protocols' are used as guidelines for conducting [[research]] and open communication

...management network will be a strategic innovation to develop harmonization protocols towards generating a rigorously monitored data repository on mitochondrial ...y is required to implement quality control systems and optimally harmonize protocols across research groups. The primary challenge is to ‘increase value and r

...oject and will contribute by training participants in the Oroboros MitoFit Laboratory and also send people form Oroboros to other institutes. ...mitochondrial defects associated with liver diseases. Application of these protocols can be used in different preparations such as isolated liver mitochondria,

| White adipose tissue mitochondrial respiration protocols - Experiments and Results discussion | Inter-laboratory harmonization of protocols to set a final consensus for the evaluation of the technical skills needed

(1) Laboratory of Translational Myology, Dept. Biomedical Sciences, University of Padua, I # Kern H, Hofer C, Mayr W, Carraro U (2009) European Project RISE: Partners, protocols, demography. Basic Appl Myol/ Europ J Translat Myol 19: 211-216.

..., respiration, hydrogen peroxide production or mt-membrane potential. SUIT protocols, calibration and instrumental service are covered as well. ...boros Instruments & Medical University of Innsbruck, D. Swarovski Research Laboratory

...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory ...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl

...uncoupler-inhibitor titration|substrate-uncoupler-inhibitor titration SUIT protocols]]) and experiments run in "Special interest groups". Short presentations of ...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory

15:35 '''Erich Gnaiger''' (D. Swarovski Research Laboratory, Department of Visceral, Transplant * [[Troppmair J|'''Troppmair Jakob''']], D. Swarovski Research Laboratory, Department of Visceral,

...issues. The active user forums and O2k-Network serve as a rich source of protocols, shared knowledge, and information on all topics related to bioenergetics a ...etry system in Antarctica! I already worked on the Oroboros in my previous laboratory in Canada and, to my opinion, it’s the best and more accurate system for

...LE) in studies of mitochondrial function. MitoEAGLE develops harmonisation protocols towards generating a data repository on mitochondrial respiratory function. ...mitochondrial capacities and create a Library of protocols: reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis

...ros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory ...-Fluorometry will be presented in overview lectures and hands-on sessions; protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membra

...3. Ten years later, ''bioRxiv'' was launched in 2013 by Cold Spring Harbor Laboratory Press in New York as a ‘free online archive and distribution service for ...ith a rigorous quality management, such as pre-publication of time stamped protocols, access to raw data of the complete data set, including calibration procedu

...ds Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscl # Tyler DD, Gonze J (1967) The preparation of heart mitochondria from laboratory animals. In: Estabrook RW, Pullman ME (eds) Meth Enzymol:75-7.