MiPNet20.12 IOC103 Manaus

MiPNet20.12 IOC103 Manaus

Manaus BR, 2015 Jul 06-07. To the heart of the Amazon. O2k-Workshop on HRR and O2k-Fluorometry, IOC103.

OROBOROS (2015-07-06) Mitochondr Physiol Network

Abstract:

2015 Jul 06-07. To the heart of the Amazon: comparative cardiac mitochondrial physiology of Amazon fish. O2k-Workshop on HRR and O2k-Fluorometry, IOC103.

• O2k-Network Lab: BR Manaus Val AL, AT Innsbruck OROBOROS

Labels:

ORO, IOC, MiPNet, 2015, Next

To the heart of the Amazon

Mitochondrial function will be compared in mitochondrial preparations of fish heart. According to the suggestion by Dr. Adalberto Luis Val, one of each of the major groups should be selected: Potamotrygonidae, Osteoglossidae, Characiformes, Siluriformes, Perciformes (Cichlidae). The results obtained with a basic SUIT protocol will be compared with mitochondrial respiratory control patterns from other species, including mouse and human heart mitochondria, to provide a basis for our understanding of mitochondrial respiratory control patterns in different species and tissues.

Venue and organization

Venue

Instituto Nacional de Pesquisas de Amazonia (INPA)

Laboratório de Ecofisiologia e Evolução Molecular (LEEM)

Ministerio da Ciencia e Tecnologia

Av. Andre Araujo, 2938

69067-375 Manaus, AM, Brazil

Local organizers and contact

Proceedings of the O2k-Workshop

IOC103 Manaus 2015-07-06

Day 1: Assembly of the O2k

From left to right: Erich Gnaiger, Reginaldo da Silva Oliveira, Derek Felipe Campos, Wadir Heinrichs Cardas, Ramon B Baptista.

Photo by Carolina Gnaiger.

Lecturers and tutors

Gnaiger Erich, CEO, OROBOROS INSTRUMENTS

Carolina Gnaiger, Assistant, OROBOROS INSTRUMENTS

SUIT protocol for the cardiac mitochondrial project

Heart homogante from various fish species will be analyzed with a basic SUIT protocol. The experimental programme is planned from July 8 to 11. 800 px 800 px

Trout heart homogenate (15 °C, 4 mg W_w/ml; 2011-10-05 A-02). Very low LEAK and ROX. No ADP limitation at D1 (1 mM) with glutamate&malate (GM), but glutamate&malate&pyruvate (GMP) was not saturated at 2 mM ADP (D2), as shown by the increase of flux when doubling the ADP concentration (4 mM; D4). No oxygen limitation. Low P/E ratio, indicating limitation of OXPHOS by the phosphorylation system. The optimum uncoupler (U; FCCP) concentration is higher than in liver homogenate (2.5 versus 1.0 µM), but 3 µM exerts already a pronounced time-dependent inhibitory effect (not due to limitation by oxygen, as shown by the lower flux after reoxygenation).

Mouse heart homogenate (37 °C) is shown for comparison.

References

On respiratory control of human heart mitochondria

Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38. »Bioblast link«