MiPNet17.03 Shredder vs Fibres
Abstract: Doerrier C, Draxl A, Wiethuechter A, Eigentler A, Gnaiger E (2015) Mitochondrial respiration in permeabilized fibres versus homogenate from fish liver and heart. An application study with the PBI-Shredder. Mitochondr Physiol Network 17.03(04):1-9. »Versions
In the present study we compared mitochondrial function of permeabilized fibres and homogenate of heart muscle of mice. In addition, respiration of trout heart homogenate preparations were compared with permeabilized fibres, and the PBI-Shredder was successfully tested with preparation of trout liver.
Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP Non-mammal: Fishes Tissue;cell: Heart, Liver Preparation: Homogenate Coupling state: LEAK, OXPHOS, ETS Substrate state: CI, CII, CI&II HRR: Oxygraph-2k, O2k-Protocol Additional: O2k-Demo, Mt-Preparation, Auxiliary, PBI-Shredder, O2k-Core