Difference between revisions of "MiPNet20.12 IOC103 Manaus"
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== To the heart of the Amazon == | == To the heart of the Amazon == | ||
[[File:Amazon 4403.JPG |left |138px |file=MiPNet20.12 IOC103|Amazon, Manaus]] | |||
Mitochondrial function will be compared in mitochondrial preparations of fish heart. According to the suggestion by Dr. Adalberto Luis Val, one of each of the major groups should be selected: Potamotrygonidae, Osteoglossidae, Characiformes, Siluriformes, Perciformes (Cichlidae). The results obtained with a basic [[SUIT protocol]] will be compared with mitochondrial respiratory control patterns from other species, including mouse and human heart mitochondria, to provide a basis for our understanding of mitochondrial respiratory control patterns in different species and tissues. | Mitochondrial function will be compared in mitochondrial preparations of fish heart. According to the suggestion by Dr. Adalberto Luis Val, one of each of the major groups should be selected: Potamotrygonidae, Osteoglossidae, Characiformes, Siluriformes, Perciformes (Cichlidae). The results obtained with a basic [[SUIT protocol]] will be compared with mitochondrial respiratory control patterns from other species, including mouse and human heart mitochondria, to provide a basis for our understanding of mitochondrial respiratory control patterns in different species and tissues. | ||
== Venue and organization == | == Venue and organization == | ||
'''Venue''' | '''Venue''' | ||
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== SUIT protocol for the cardiac mitochondrial project == | == SUIT protocol for the cardiac mitochondrial project == | ||
Heart homogante from various fish species will be analyzed with a basic SUIT protocol. The experimental programme is planned from July 8 to 11. | Heart homogante from various fish species will be analyzed with a basic SUIT protocol. The experimental programme is planned from July 8 to 11. | ||
[[File:Trout-heart 2011-10-05 A-02.jpg |800 px]] | [[File:Trout-heart 2011-10-05 A-02.jpg |800 px]] | ||
[[File:Mouse-heart_2011-11-16_C-02.jpg |800 px]] | [[File:Mouse-heart_2011-11-16_C-02.jpg |800 px]] | ||
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Mouse heart homogenate (37 °C) is shown for comparison. | Mouse heart homogenate (37 °C) is shown for comparison. | ||
=== References === | |||
* On respiratory control of human heart mitochondria | * On respiratory control of human heart mitochondria | ||
:: Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38. »[[Lemieux 2011 Int J Biochem Cell Biol |Bioblast link]]« | :: Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38. »[[Lemieux 2011 Int J Biochem Cell Biol |Bioblast link]]« | ||
* » [[O2k-Publications: Comparative MiP;environmental MiP |Comparative mitochondrial physiology]] | |||
* » [[O2k-Publications: Fishes]] | |||
* » [[MiPNet17.03 Shredder vs Fibres]] | |||
=== Recommended IOC reading === | |||
== Recommended reading == | |||
Please click »[[IOC Recommended Reading| here]]« | Please click »[[IOC Recommended Reading| here]]« | ||
Revision as of 12:58, 6 July 2015
MiPNet20.12 IOC103 Manaus
Manaus BR, 2015 Jul 06-07. To the heart of the Amazon. O2k-Workshop on HRR and O2k-Fluorometry, IOC103. |
OROBOROS (2015-07-06) Mitochondr Physiol Network
Abstract:
2015 Jul 06-07. To the heart of the Amazon: comparative cardiac mitochondrial physiology of Amazon fish. O2k-Workshop on HRR and O2k-Fluorometry, IOC103.
• O2k-Network Lab: BR Manaus Val AL, AT Innsbruck OROBOROS
Labels:
ORO, IOC, MiPNet, 2015, Next
To the heart of the Amazon
Mitochondrial function will be compared in mitochondrial preparations of fish heart. According to the suggestion by Dr. Adalberto Luis Val, one of each of the major groups should be selected: Potamotrygonidae, Osteoglossidae, Characiformes, Siluriformes, Perciformes (Cichlidae). The results obtained with a basic SUIT protocol will be compared with mitochondrial respiratory control patterns from other species, including mouse and human heart mitochondria, to provide a basis for our understanding of mitochondrial respiratory control patterns in different species and tissues.
Venue and organization
Venue
- Instituto Nacional de Pesquisas de Amazonia (INPA)
- Laboratório de Ecofisiologia e Evolução Molecular (LEEM)
- Ministerio da Ciencia e Tecnologia
- Av. Andre Araujo, 2938
- 69067-375 Manaus, AM, Brazil
Local organizers and contact
Lecturers and tutors
Gnaiger Erich, CEO, OROBOROS INSTRUMENTS
- Carolina Gnaiger, Assistant, OROBOROS INSTRUMENTS
SUIT protocol for the cardiac mitochondrial project
Heart homogante from various fish species will be analyzed with a basic SUIT protocol. The experimental programme is planned from July 8 to 11. 800 px 800 px
Trout heart homogenate (15 °C, 4 mg Ww/ml; 2011-10-05 A-02). Very low LEAK and ROX. No ADP limitation at D1 (1 mM) with glutamate&malate (GM), but glutamate&malate&pyruvate (GMP) was not saturated at 2 mM ADP (D2), as shown by the increase of flux when doubling the ADP concentration (4 mM; D4). No oxygen limitation. Low P/E ratio, indicating limitation of OXPHOS by the phosphorylation system. The optimum uncoupler (U; FCCP) concentration is higher than in liver homogenate (2.5 versus 1.0 µM), but 3 µM exerts already a pronounced time-dependent inhibitory effect (not due to limitation by oxygen, as shown by the lower flux after reoxygenation).
Mouse heart homogenate (37 °C) is shown for comparison.
References
- On respiratory control of human heart mitochondria
- Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38. »Bioblast link«
Recommended IOC reading
Please click » here«
» MitoPedia: Respiratory states
O2k-Workshops are listed as MitoGlobal Events.