Difference between revisions of "MitoEAGLE protocols, terminology, documentation"
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:::: It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease. | :::: It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease. | ||
== WG1 Participants == | |||
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::: '''Best practices''' for implementation of analytical and process standards. | ::: '''Best practices''' for implementation of analytical and process standards. | ||
Revision as of 14:50, 27 April 2016
News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE protocols, terminology, documentation
WG1
- Standard operating procedures and user requirement document: Protocols, terminology, documentation
WG1 Description
- The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.
Respirometric reference protocols
- A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also Fig. 3).
Interlaboratory proficiency test
- Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
Instrumental platforms
- Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.
Nomenclature
- Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
Knowledge management system
- With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
- The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
- Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
- It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
WG1 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Australia | Sydney | Krycer James R |
Australia | Queensland | Holland Olivia J |
Australia | Newtown, Sydney | Stocker Roland |
Australia | Geelong, Victoria | McKenzie Matthew |
Australia | Queensland | Fisher Joshua J |
Australia | Melbourne | Stepto Nigel K |
Australia | Queensland | Dong Lan-Feng |
Australia | Melobourne | Botella Javier |
Australia | Victoria | Van Bergen Nicole J |
Australia | Southport | Vidimce Josif |
Australia | Melbourne | Ferri Alessandra |
Australia | Melbourne | Whitfield Jamie |
Australia | Queensland | Radenkovic Filip |
Australia | Clayton | Wolff Jonci Nikolai |
Australia | Melbourne, VIC | Muccini Anna Maria |
Australia | Melbourne | Kuang Jujiao |
Australia | Melbourne | Saner Nicholas |
Australia | Syndey | Ton Riccardo |
Australia | Nedlands | Perks Kara L |
Australia | Nedlands | Filipovska Aleksandra |
Australia | Victoria | Winwood-Smith Hugh |
Australia | Melbourne | Hewakapuge Sudinna |
Australia | Burwood | Trewin Adam J |
Australia | Melbourne | Lionett S |
Australia | Clayton Victoria | Alton Lesley |
Australia | Sydney | Hoehn Kyle L |
Australia | Melbourne | Cassar Samantha |
Australia | Parkville | Hardee Justin P |
Australia | Melbourne | Coughlan Melinda T |
Australia | Melbourne | Genders Amanda J |
Austria | Innsbruck | Laner Verena |
Austria | Innsbruck | Keller Markus A |
Austria | Innsbruck | Nindl Veronika |
Austria | Innsbruck | Gnaiger Erich |
Austria | Salzburg | Kofler B |
Austria | Innsbruck | Thapa Maheshwor |
Austria | Innsbruck | Plangger Mario |
Austria | Innsbruck | Schartner Melanie |
Austria | Innsbruck | Passrugger Manuela |
Austria | Innsbruck | Goebel G |
Austria | Innsbruck | Di Marcello Marco |
Austria | Vienna | Kozlov Andrey V |
Austria | Innsbruck | Bergmeister Lisa |
Austria | Innsbruck | Antunes Diana |
Austria | Kapferer Werner | |
Austria | Innsbruck | Burtscher Martin |
Austria | Innsbruck | Klocker Helmut |
Austria | Innsbruck | Gallee Leon |
Austria | Innsbruck | Karabatsiakis Alexander |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Perez Valencia Juan Alberto |
Austria | Aldrans | Haider Markus |
Austria | Innsbruck | Hoppel Florian |
Austria | Innsbruck | Schmitt Sabine |
Austria | Innsbruck | Schwarzer Christoph |
Austria | Innsbruck | Meszaros Andras T |
Austria | Klosterneuburg | Sazanov Leonid A |
Austria | Innsbruck | Doerrier Carolina |
Austria | Innsbruck | Cecatto Cristiane |
Austria | Innsbruck | Sobotka Ondrej |
Austria | Innsbruck | Garcia-Souza Luiz F |
Belgium | Brussels | Sonveaux P |
Belgium | Leuven, Flanders | Baekelandt V |
Belgium | Liege | Votion Dominique-Marie |
Belgium | Namur | Arnould Thierry |
Belguim | Sart Tilman (Liège) | Kruse C |
Brasil | Porto Alegre | Haas Clarissa |
Brasilia | Limeira | Ropelle Eduardo R |
Brazil | Rio de Janeiro | Oliveira Marcus F |
Brazil | São Paulo | Crispim Marcell |
Brazil | Sao Paulo | Vercesi Anibal E |
Brazil | Sao Paulo | Silber Ariel Mariano |
Brazil | Santa Maria - RS | Goncalves Debora Farina |
Brazil | Santa Maria - Rio Grande do Sul | Soares Felix Alexandre Antunes |
Brazil | Rio de Janeiro | Vieyra Adalberto |
Brazil | São Paulo | Kowaltowski Alicia J |
Brazil | Rio de Janeiro | Galina Antonio |
Brazil | Jaboticabal | Oliveira Marcos Tulio |
Canada | Montréal | Breton Sophie |
Canada | Edmonton | Lucchinetti Eliana |
Canada | Hamilton | Scott Graham R |
Canada | Ottawa | Harper Mary-Ellen |
Canada | Hamilton | Tarnopolsky Mark A |
Canada | Montreal, Quebec | Bergdahl Andreas |
Canada | Guelph | Holloway Graham P |
Canada | Saint John | Pulinilkunnil Thomas |
Canada | Edmonton | Han Woo Hyun |
Canada | Vancouver, BC | Tausan Daniel |
Canada | Ottawa | Darveau Charles-A |
Canada | Quebec | Joseph Vincent |
Canada | Charlottetown | Adiele Reginald C |
Canada | Rimouski | Blier Pierre U |
Canada | Moncton, NB | Pichaud Nicolas |
Canada | Antigonish | Weir M |
Canada | Vancouver | Bovard Josh |
Canada | Edmonton (Alberta) | Lemieux Helene |
Canada | Edmonton | Zaugg Michael |
Canada | Winnipeg | Chowdhury Subir Roy |
Canada | Rimouski | Munro Daniel |
Chile | Coyhaique | Jana Prado Fabian |
China | Xi`an | Liu Jiankang |
China | Tianjin | Zhang Yong |
China | Pukou, Nanjing, | Gan Zhenji |
China | Fuzhou, Fujian Province | Chen Qi |
Croatia | Split | Ljubkovic M |
Croatia | Zagreb | Saric A |
Croatia | Zagreb | Curik I |
Croatia | Zagreb | Rubelj Ivica |
Czech Republic | Hradec Kralove | Stankova Pavla |
Czech Republic | Plzen -Severni predmesti | Jedlicka Jan |
Czech Republic | Hradec Kralove | Cervinkova Zuzana |
Czech Republic | Pardubice | Majtnerova Pavlina |
Czech Republic | Ceske Budejovice | Zikova Alena |
Czech Republic | Pardubice | Rousar Tomas |
Czech Republic | Prague | Drahota Zdenek |
Czech Republic | Pardubice | Handl Jiri |
Czech Republic | Prague | Krizova J |
Czech Republic | Praha 2 | Danhelovska Tereza |
Czech Republic | Prague | Houstek Josef |
Czech Republic | Hradec Kralove | Endlicher Rene |
Czech Republic | Vestec | Ezrova Zuzana |
Czech Republic | Vestec | Rohlena Jakub |
Czech Republic | Praha 2 | Zdrazilova Lucie |
Czech Republic | Hradec Kralove | Kucera Otto |
Czech Republic | Vestec | Davidova Eliska |
Czech Republic | Prague 10 | Urban Tomas |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Aarhus N | Jespersen Nichlas Riise |
Denmark | Copenhagen | Dela Flemming |
Denmark | Copenhagen | Nielsen B |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Aarhus N | Boetker Hans Erik |
Denmark | Copenhagen | Gonzalez-Franquesa Alba |
Denmark | Copenhagen | Kraunsoee R |
Denmark | Copenhagen | Larsen Steen |
Denmark | Copenhagen | Soendergaard SD |
Denmark | Copenhagen N | Mortensen OH |
Denmark | Copenhagen N | Maise Chroeis K |
Denmark | Copenhagen N | Scheibye-Knudsen Morten |
Denmark | Roskilde | Dalgaard Louise T |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Egypt | Cairo | Ali Sameh S |
Estonia | Tallinn | Tepp Kersti |
Estonia | Tallinn | Puurand Marju |
Estonia | Tartu | Paju Kalju |
Estonia | Tallinn | Laasmaa Martin |
Estonia | Tallinn | Klepinin Aleksandr |
Estonia | Tallinn | Karro N |
Estonia | Tallinn | Shevchuk Igor |
Estonia | Tallinn | Truu Laura |
Estonia | Tallinn | Klepinina Lyudmila |
Estonia | Tallinn | Vendelin Marko |
Estonia | Tallinn | Kaambre Tuuli |
Finland | Turku | Stier Antoine |
Finland | Helsinki | Jackson Christopher Benjamin |
Finland | Helsinki | Suomalainen Wartiovaara Anu |
Finland | Jyvaeskylae | Kainulainen Heikki |
France | Bordeaux | Amoedo Nivea Dias |
France | Maisons-Alfort Cedex | Prola Alexandre |
France | Bordeaux | Sarlak Saharnaz |
France | Gueguen Naig | |
France | Paris | Armand Anne-Sophie |
France | Bordeaux cedex | Mourier A |
France | Bordeaux cedex | Rossignol Rodrigue |
France | Plouzané | Salin Karine |
France | Tours | Dumas Jean-Francois |
France | Martinique | Neviere Remi |
France | Nouzilly | Collin-Chenot Anne |
France | Angers | Spinazzi Marco |
France | Angers | Procaccio Vincent |
France | Paris | Bouillaud Frederic |
France | Lille | Montaigne David |
France | Bordeaux | Mazat Jean-Pierre |
France | Montpellier | Wrutniak-Cabello Chantal |
France | Martinique | Cano Sanchez Maria Consolacion |
Germany | Cologne | Pesta Dominik |
Germany | Biberach an der Riß | Nold V |
Germany | Tuebingen | Kappler Lisa |
Germany | Frankfurt | Warnsmann Verena |
Germany | Wilhelmshaven | Salmon Pablo |
Germany | Duesseldorf | Piel Sarah |
Germany | Cologne | Ho Dieu Hien |
Germany | Giessen | Schulz Rainer |
Germany | Ulm | Schaefer PM |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Jena | Szibor Marten |
Germany | Cologne | Maciej Sarah |
Germany | Hannover | Das Anibh Martin |
Germany | Mainz | Methner Axel |
Germany | Munich | Zischka Hans |
Germany | Düsseldorf | Goy C |
Germany | Frankfurt | Hamann Andrea |
Germany | Magdeburg | Schoenfeld Peter |
Germany | Marburg | Vogt Sebastian |
Germany | Cologne | Wiesner Rudolf J |
Germany | Wickert Anika | |
Germany | Duesseldorf | Roden Michael |
Germany | Gießen | Silaidos Carmina |
Germany | Freiburg | Schuele R |
Germany | Cologne | Trifunovic Aleksandra |
Germany | Bremerhaven | Mark Felix Christopher |
Germany | Marburg | Ramzan Rabia |
Germany | Frankfurt am Main | Viel Christian |
Germany | Rostock | Sokolova Inna |
Germany | Duesseldorf | Haendeler Judith |
Germany | Doermann Niklas | |
Germany | Giessen | Sommer Natascha |
Germany | Duesseldorf | Jelenik T |
Germany | Mainz | Maull Felicia |
Germany | Frankfurt | Osiewacz Heinz D |
Germany | Gießen | Grewal Rekha |
Germany | Neuherberg | Einer C |
Greece | Athens | Trougakos Ioannis P |
Greece | Thessaloniki | Lazou Antigone |
Greece | Heraklion | Tavernarakis Nektarios |
Greece | 15771 | Andreadou Ioanna |
Greece | Athens | Gumeni Sentiljana |
Hungary | Budapest | Chinopoulos Christos |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Horvath G |
Hungary | Budapest | Tretter Laszlo |
Hungary | Szeged | Ferdinandy Peter |
Hungary | Budapest | Komlodi Timea |
Hungary | Szeged | Boros Mihaly |
India | New Delhi | Vincent Vinnyfred |
India | Lucknow | Gayen Jiaur |
India | Haryana | Chakrabarti Sasanka |
India | Hyderabad | Jha Rajan Kumar |
India | Varanasi | Sonkar Vijay K |
India | Kollam | Suravajhala Prashanth |
India | New Delhi | Thakkar Himani |
Iran | Tehran | Safaei Zahra |
Iran | Tehran | Shirazi Reza |
Ireland | Dublin | Porter Richard K |
Ireland | Dublin | O'Gorman Donal |
Israel | Jerusalem | Saada Reisch Ann |
Israel | Rishon Le Zion | Hachmo Yafit |
Israel | Zrifin | Sova Marina |
Israel | Beer Sheva | Mishmar Dan |
Italia | Udine | Salvadego Desy |
Italien | Padova PD | Szabo Ildiko |
Italy | Udine | Grassi B |
Italy | Catania | Messina Angela |
Italy | Bologna | Genova Maria Luisa |
Italy | Campobasso | Pallotta Maria Luigia |
Italy | Catania | De Pinto Vito |
Italy | Milan | Clementi Emilio |
Italy | Rome | Scatena Roberto |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Bologna | Paterlini S |
Italy | Milano | Bottoni P |
Italy | Ancona | Battino Maurizio |
Italy | Bologna | Porcelli AM |
Italy | Padova | Viscomi Carlo |
Italy | Padova | Bernardi Paolo |
Italy | Bolzano | Zanon Alessandra |
Italy | Bologna | Gasparre G |
Italy | Bolzano/Bozen | Volani Chiara |
Italy | Monserrato | Isola Raffaella |
Italy | Bari | Attimonelli M |
Italy | St. Lorenzen | Harrison David K |
Italy | Catania | Magri Andrea |
Japan | Sapporo | Matsumoto J |
Japan | Itabashi-ku Tokyo | Tanaka Masashi |
Kitzbühel | Innsbruck | Fischer Michael J |
Korea | Seoul | Pak Youngmi Kim |
Korea | Incheon | Kwak Hyo Bum |
Korea (South) | Seoul | Lee Hong Kyu |
Latvia | Riga | Dambrova Maija |
Latvia | Riga | Vilks Karlis |
Latvia | Riga | Svalbe Baiba |
Latvia | Riga | Zvejniece Liga |
Latvia | Riga | Stelfa Gundega |
Latvia | Riga | Liepins Edgars |
Latvia | Riga | Volska Kristine |
Latvia | Riga | Jansone Baiba |
Latvia | Riga | Inashkina I |
Luxembourg | Strassen | Devaux Yvan |
Malaysia | Kuala Lumpur | Hassan Hazirah |
Malta | Msida | Vella Joanna |
Mexico | Mexico City | Aparicio Trejo OE |
Mexico | Tlalpan | Rodriguez-Enriquez Sara |
México | San Pedro Garza García, Nuevo Leon | Garcia-Rivas Gerardo |
Netherlands | GA Wageningen | Grefte Sander |
Netherlands | Maastricht | Grevendonk L |
New Zealand | Auckland | Ward Marie Louise |
New Zealand | Wellington | Berridge Michael V |
New Zealand | Auckland | Kaur Sarbjot |
New Zealand | Auckland | Hickey Anthony J |
Norway | Bergen | Dyrstad Sissel E |
Norway | Trondheim | Lerfall Joergen |
Norway | Trondheim | Grill V |
Norway | Bergen | Berge Rolf K |
Norway | Bergen | Hoel Fredrik |
Norway | Bergen | Pettersen Nitschke Ina Katrine |
Norway | Bergen | Haavik Jan |
Norway | Trondheim | Smenes Benedikte Therese |
Norway | Oslo | Eide L |
Norway | Trondheim | Rognmo O |
Norway | Bergen | Tronstad Karl Johan |
Norway | Tromsoe | Boardman Neoma T |
Norway | Bergen | Roesland Gro Vatne |
Norway | Bergen | Blindheim Dan Filip |
Poland | Warsaw | Wieckowski Mariusz R |
Poland | Warsaw | Bednarczyk Piotr |
Poland | Poznan | Jarmuszkiewicz Wieslawa |
Poland | Gdańsk | Kaczor Jan Jacek |
Poland | Warsaw | Sek Aleksandra |
Poland | Warsaw | Karkucinska-Wieckowska Agnieszka |
Poland | Warsaw | Zablocki Krzysztof |
Poland | Warsaw | Wasilewski M |
Poland | Lodz | Watala Cezary |
Poland | Warsaw | Dymkowska Dorota |
Poland | Lodz | Labieniec-Watala Magdalena |
Poland | Warszawa | Jaskiewicz Anna |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Warsaw | Szewczyk Adam |
Poland | Krakow | Dembinska-Kiec Aldona |
Portugal | Cantanhede | Rodrigues Ana Sofia |
Portugal | Porto | Crisostomo Luis |
Portugal | Porto | Moreira Bruno P |
Portugal | Porto | Silva Ana Maria |
Portugal | Aveiro | Vitorino Rui Miguel Pinheiro |
Portugal | Cantanhede | Amorim Ricardo |
Portugal | Coimbra | Dias Candida |
Portugal | Porto | Beleza Jorge |
Portugal | Cantanhede | Teodoro Joao Soeiro |
Portugal | Porto | Alves Marco G |
Portugal | Coimbra | Duarte Filipe Valente |
Portugal | Coimbra | Santos Diana |
Portugal | Coimbra | Grilo Luis |
Portugal | Coimbra | Oliveira Paulo J |
Portugal | Coimbra | Minuzzi Luciele M |
Portugal | Lisboa | Macedo Maria Paula |
Portugal | Porto | Oliveira Jorge |
Portugal | Aveiro | Ferreira Rita Maria P |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Coimbra | Rolo Anabela Pinto |
Qatar | Doha | Guarch Meritxell Espino |
Republic of Ghana | Cape Coast | Tei BN |
Republic of Korea | Daejeon | Park SH |
Republic of Korea | Busan | Kim Hyoung Kyu |
Republic of Korea | Busan | Han Jin |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Serbia | Novi Sad | Andric Silvana |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Republic of Serbia | Belgrade | Lalic Nebojsa M |
Russia | Yoshkar-Ola | Dubinin M |
Russia | Moscow | Vinogradov Andrey D |
Russia | Moscow | Pavlova Nadia |
Serbia | Belgrade | Savkovic U |
Serbia | Belgrade | Mandic M |
Serbia | Belgrade | Misirkic Marjanovic Maja |
Serbia | Belgrade | Djordjevic M |
Singapore | Singapore | Singh Brijesh Kumar |
Singapore | Singapore | Hausenloy Derek J |
Slovakia | Bratislava 45 | Ferko Miroslav |
Slovakia | Košice | Cizmarova Beata |
Slovakia | Bratislava | Sumbalova Zuzana |
Spain | Barcelona | Goncalo Teixeira da Silva Rui |
Spain | Sevilla | Bustos Matilde |
Spain | Barcelona | Bravo-Sagua Roberto |
Spain | Madrid | Moran M |
Spain | A Coruña | Blanco FJ |
Spain | Granada | Hidalgo-Gutierrez A |
Spain | Barcelona | Zorzano Antonio |
Spain | Barcelona | Gil J |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Valencia | Casado Pinna Marta |
Spain | Barcelona | Perales Jose Carles |
Spain | Barcelona | Gama Perez Pau |
Spain | Granada | Quiles JL |
Spain | Barcelona | Garcia-Roves Pablo Miguel |
Spain | Madrid | Hernansanz-Agustin Pablo |
Spain | Barcelona | De la Torre Lara J |
Spain | A Coruña | Mayan MD |
Spain | Salamanca | Almeida Angeles |
Spain | Las Palmas de Gran Canaria | Calbet Jose AL |
Spain | Madrid | Rial Eduardo |
Spain | Madrid | Aragones Lopez J |
Sweden | Stockholm | Cardinale Daniele A |
Sweden | Stockholm | Larsen Filip J |
Sweden | Stockholm | Shabalina Irina G |
Sweden | Gothenburg | Bhuvanachandran NS |
Sweden | Lund | Ehinger Johannes K |
Sweden | Stockholm | Nedergaard J |
Switzerland | Lausanne | Canto Alvarez Carles |
Switzerland | Lausanne | Sandi Carmen |
Switzerland | Lausanne | Gebara E |
Switzerland | Bern | Wyss RK |
Switzerland | Lausanne | Lagarrigue Sylviane |
Switzerland | Basel | Fornaro Mara |
Switzerland | Zurich | Gorr Thomas A |
Switzerland | Lausanne | Burtscher Johannes |
Switzerland | Lausanne | Donnelly Chris |
Switzerland | Lausanne | Morato Fornaguera L |
Switzerland | Zürich | Zaugg Kathrin |
Switzerland | Lausanne | Weger M |
Switzerland | Basel | Bouitbir Jamal |
Switzerland | Lausanne | Zanou Nadege |
Switzerland | Lausanne | Amati Francesca |
Switzerland | Lausanne | Place Nicolas |
Switzerland | Bern | Nuoffer Jean-Marc |
Switzerland | Lausanne | Zalachoras I |
Taiwan | Changhua City | Wei Yau-Huei |
The Netherlands | BT Amsterdam | Wuest Rob CI |
The Netherlands | Groningen | Bakker Barbara M |
The Netherlands | Delft | McMillan Duncan GG |
The Netherlands | Maastricht | Schrauwen Patrick |
The Netherlands | Maastricht | Nabben Miranda |
UK | London | Rodriguez Enrique |
UK | London | Lane Nick |
UK | Cambridge | Brown Guy C |
UK | Canterbury, Kent | Gourlay Campbell W |
UK | London | Bettinazzi Stefano |
USA | Orlando | Sparks Lauren M |
USA | Little Rock | Borsheim Elisabet |
USA | Rochester | Nair K Sreekumaran |
USA | Little Rock | MacMillan-Crow Lee Ann |
USA | Gainesville | Neyroud D |
USA | Orlando | Pino Maria F |
USA | La Jolla | Schilling Jan M |
USA | Gainesville | Leeuwenburgh Christiaan |
USA | Aurora | Moreau Kerrie |
USA | Milwaukee | Kandel Sunil Mani |
USA | Corvallis | Newsom Sean A |
USA | Columbus | Sims Carrie A |
USA | Philadelphia | Towheed Atif |
USA | Norfolk | Lai Nicola |
USA | New Haven | Cavalcanti-de-Albuquerque Joao Paulo |
USA | Cleveland | Tandler Bernard |
USA | Kansas City | Thyfault John P |
USA | Rochester | Lanza Ian R |
USA | Little Rock | Parajuli Nirmala |
USA | Puskarich Michael A | |
USA | Indianapolis | Brozinick Joseph T |
USA | Berkeley | Williams Caroline M |
USA | Kansas City | Swerdlow Russell H |
USA | Winston-Salem | Gonzalez-Armenta Jenny L |
USA | Colorado Springs | Jacobs RA |
USA | New York | Galkin Alexander |
USA | Atlanta | Jang Young Charles |
USA | La Jolla | Valentine Joseph Marco |
USA | Fayetteville | Iyer Shilpa |
USA | Iowa City | Chaurasia Bhagirath |
USA | Nashville | Wasserman David H |
USA | Fairbanks | O'Brien Kristin |
USA | Winston-Salem | Ahn Bumsoo |
USA | San Diego | Patel Hemal H |
USA | Bethesda | Fessel Joshua Patrick |
USA | Baton Rouge | Irving Brian A |
USA | St. Louis | Abumrad Nada A |
USA | Baltimore | Gonzalez-Freire Marta |
USA | Louisville | Skolik Robert A |
USA | Stillwater | Davis Michael S |
USA | Fayetteville | Rao RR |
USA | Baton Rouge | Hand Steven C |
USA | Birmingham | Moellering Douglas R |
USA | Philadelphia | Ganetzky Rebecca |
USA | Pittsburgh | Jurczak Michael J |
USA | Wakefield | Varricchio Frederick |
USA | San Diego; La Jolla | Molina Anthony JA |
USA | Philadelphia | Falk Marni J |
USA | Fort Collins | Li Puma Lance C |
USA | Akron | Cohen Bruce H |
USA | East Lansing | Zhang Yizhu |
USA | Davis | Hellgren Kim T |
USA | Winston-Salem | Tomar Dhanendra |
USA | Los Angeles | Kwast Kurt E |
USA | Davis | Adams Sean H |
USA | East Lansing | Bazil Jason N |
USA | Greenville | Neufer P Darrell |
USA | Provo | Hancock Chad R |
USA | St. Louis | Pietka Terri A |
USA | Philadelphia | Jang David H |
USA | Aurora | Ford Ellen |
USA | Ann Arbor | Tyrrell Daniel J |
USA | Louisville | Menze Michael A |
USA | San Diego | Cannon Daniel T |
USA | Chicago | Dayanidhi Sudarshan |
USA | Pittsburgh | Prochownik Edward V |
USA | Galveston | Durham William J |
USA | Warminster | Orynbayeva Zulfiya |
USA | Corvallis | Batterson Philip M |
USA | Omaha | Kim Julian KS |
USA | Milwaukee | Dash Ranjan K |
USA | Cambridge | Wohlwend Martin |
USA | Needham | Brown David A |
USA | Bethesda | Sharma Pushpa |
USA | Denton | Dzialowski Edward M |
USA | Portland | Garlid Keith D |
USA | Orlando | Coen Paul M |
USA | Miami | Fontanesi F |
USA | Boston | Kristal Bruce S |
USA | Orlando | Goodpaster Bret H |
USA | Denver | Van Hove Johan |
USA | Aurora | Sparagna Genevieve C |
USA | Iowa City | Wagner Brett A |
... further results |
WG1 Management
Tasks
- Opening of the database to the research public for data search and moderated entry of new data sets.
- Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module (with WG5).
- Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
- Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report (move to WG5).
- Instrumental platform comparison with reference sample applying comparable measurements.
- Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology (with WG5).
- Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
- MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
- MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
- Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
- Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
- Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation (move to WG5).
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE (move to WG5).
Milestones
- Data sets entered in standardized format and database opened for the public
- Protocols for ring-tests and standard analysis and report scheme defined
- MITOEAGLE proficiency training workshop finished (move to WG5)
- Analysis is completed and joint publication submitted (with WG5)
- Publication of paper on concepts and terminology (with WG5)
- Library of protocols online
- Publication of recommended protocols and procedures
- Guidelines for acquisition, evaluation and data documentation available (with WG5)
- MITOEAGLE data management system finished
- MITOEAGLE database ready for use
- Software modifications incorporated following extensive tests
- Publication of MITOEAGLE-KMP
- Recommendations on data acquisition, evaluation and management published (with WG5)
- Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published (move to WG5)
Deliverables
- Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples (with WG5)
- A database on mt fitness evaluated with human cells and tissues
- Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation (move to WG5)
- Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
- An expanded MITOEAGLE database
- A joint publication documenting the results of the MITOEAGLE-PT (with WG5)
- A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology (with WG5)
- A library of protocols for sample preparation and examination by respirometry
- A multi-authored publication with recommendations of comparable standard protocols and procedures (with WG5)
- Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest
- The MITOEAGLE data management system
- A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
- A joint publication on the use of the MITOEAGLE database and management system (with WG5)
- An education programme for the use of MITOEAGLE database and management system (move to WG5).
Projects in progress
- Interlaboratory proficiency ring test
- Testing of software components
- Concepts and terminology on mitochondrial physiology
- Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
- Data management system (DMS)
- Database
Next steps
- Best practices for implementation of analytical and process standards.
References
- General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005