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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country City Contact Armenia Yerevan Trchounian K Austria Innsbruck Gnaiger Erich Austria Innsbruck Fischer Christine Austria Innsbruck Meszaros Andras T Austria Innsbruck Torres-Quesada Omar Austria Innsbruck Garcia-Souza Luiz F Austria Innsbruck Doerrier Carolina Austria Innsbruck Karabatsiakis Alexander Austria Innsbruck Cardoso Luiza HD Austria Innsbruck Hoppel Florian Belgium Namur Detraux Damien Belgium Namur Arnould Thierry Belgium Leuven Bird Matthew Belgium Liege Votion Dominique-Marie Belgium Namur Renard P Bosnia and Herzegovina Tuzla Hajrulahovic Anesa Bosnia and Herzegovina Tuzla Jusic Amela Brazil Sao Paulo Verdaguer Ignasi Bofill Brazil Sao Paulo Dominguez-Amorocho OA Brazil Sao Paulo Alencar Mayke Bezerra Brazil São Paulo Crispim Marcell Croatia Zagreb Rubelj Ivica Czech Republic Hradec Kralove Vernerova Andrea Czech Republic Prague Mracek Tomas Czech Republic Hradec Kralove Maseko Tumisang Edward Czech Republic Vestec Zobalova Renata Czech Republic Prague Hansikova Hana Czech Republic Pilsen Markova Michaela Czech Republic Pilsen Kripnerova M Czech Republic Prague Fisar Zdenek Czech Republic Pilsen Kuncova J Czech Republic Prague Pecina Petr Czech Republic Prague 4 Bajzikova Martina Czech Republic Prague Elkalaf Moustafa Czech Republic and Australia Prague and Southport Neuzil Jiri Czech rep Hradec Kralove Falaye T Denmark Copenhagen Desler C Denmark Malov Hey-Mogensen M Denmark Hvidovre Nehlin Jan O Denmark Copenhagen Rasmussen LJ Denmark Copenhagen N Mortensen OH Denmark Aarhus Rattan Suresh IS Egypt Cairo Ali Sameh S Egypt Cairo Abdel-Rahman Engy Ali Estonia Tallinn Truu Laura Estonia Tartu Grellier T Estonia Tallinn Klepinina Lyudmila Finland Jyvaeskylae Lehti Maarit Finland Turku Stier Antoine Finland Helsinki Pirinen E France Paris Petit Patrice X France Bron Rizwan Alam M France Bordeaux cedex Mourier A Germany Ulm Hartmann C Germany Freising Klingenspor Martin Germany Leipzig Ost Mario Germany Regensburg Renner-Sattler Kathrin Germany Duesseldorf Piel Sarah Germany Neuherberg Borchard Sabine Germany Freising Keppner Gloria Germany Giessen Eckert Gunter P Germany Ulm Kolassa Iris Germany Gießen Silaidos Carmina Germany Düsseldorf Prigione Alessandro Germany Munich Jastroch M Germany Hamburg Singer Dominique Germany Biberach an der Riß Nold V Germany Freising Fromme Tobias Germany Duesseldorf Eckers A Germany Mainz Vujacic-Mirski Ksenija Germany Freising Schoettl T Germany Ulm Calzia Enrico Germany Ulm Schaefer PM Germany Mainz Kalinovic S Germany Magdeburg Vieira Ligo Teixeira Camila Germany Freising-Weihenstephan Musiol Eva Greece Athens Trougakos Ioannis P Hungary Debrecen Verebne Tar K Hungary Budapest Tretter Laszlo Hungary Budapest Komlodi Timea Ireland Dublin Porter Richard K Ireland Sligo Murphy J Ireland Dublin Karavyraki Marilena Israel Rishon Le Zion Hachmo Yafit Israel Jerusalem Saada Reisch Ann Israel Zrifin Sova Marina Italy Padova Fernandez-Vizarra Erika Italy Bolzano Pichler Irene Italy Bolzano Castelo Rueda Maria Paulina Italy Bolzano/Bozen Volani Chiara Italy Bologna Paterlini S Italy Bologna Porcelli AM Italy Ancona Battino Maurizio Italy Bologna Gasparre G Italy Rome Scatena Roberto Italy Milano Bottoni P Italy Verona Calabria Elisa Italy Bologna Genova Maria Luisa Japan Sapporo Shirakawa R Latvia Riga Makarova Elina Latvia Riga Kidere Dita Malaysia Kuala Lumpur Abdul Karim Norwahidah Malta Msida Vella Joanna Netherlands Nijmegen Koopman Werner JH Netherlands Wageningen Keijer Jaap Netherlands Groningen Rots MG Norway Oslo Eide L Norway Oslo Nikolic N Norway Oslo Rustan AC Norway Oslo Lund Jenny Norway Bergen Tronstad Karl Johan Palestine Ramallah Stiban Johnny Poland Poznan Rybacka-Mossakowska Joanna Poland Lodz Siewiera Karolina Poland Poznan Michalak Slawomir Poland Warsaw Wasilewski M Poland Krakow Kaczara P Poland Warsaw Dymkowska Dorota Poland Poznań Ludziejewska A Poland Poznan Swiniuch Daria Poland Lodz Watala Cezary Poland Warsaw Kampa Rafal Pawel Poland Lodz Labieniec-Watala Magdalena Poland Warsaw Sek Aleksandra Portugal Coimbra Carvalho Eugenia Portugal Porto Bernardino RL Portugal Porto Alves Marco G Portugal Coimbra Pereira Susana P Portugal Cantanhede Pinho Sonia A Portugal Porto Beleza Jorge Portugal Catanhede Pereira da Silva Grilo da Silva Filomena Portugal Coimbra Palmeira Carlos Portugal Porto Carrageta D Portugal Porto Magalhaes Jose Portugal Porto Martins Ana Dias Portugal Porto Crisostomo Luis Portugal Porto Moreira Bruno P Portugal Cantanhede Bento Guida Jose Freitas Portugal Porto Oliveira Pedro Fontes Portugal Covilhã Dias Tania R Republic of Korea Daejeon Park SH Republic of Korea Yuseong-gu Daejeon Quang Don T Republic of Serbia Belgrade Pavlovic Kasja Republic of Serbia Belgrade Krako Jakovljevic Nina Romania Timisoara Lelcu Theia Romania Timisoara Muntean Danina M Romania Timisoara Betiu AM Romania Timisoara Avram Vlad F Romania Timisoara Lighezan R Romania Timisoara Danila Maria-Daniela Russia Moscow Itkis YS Russia Moscow Tsygankova P Russia Moscow Krylova T Saudi Arabia Giza Kotb IA Serbia Belgrade Vukojevic M Serbia Belgrad Markovic Ivanka Serbia Belgrade Misirlic-Dencic S Serbia Belgrade Isakovic A Serbia Belgrade Ivanovic Tijana Serbia Belgrade Jeremic M Slovakia Bratislava Nemec Michal Slovakia Bratislava Ukropec J Slovakia Košice Cizmarova Beata Slovakia Bratislava Ukropcova B Slovakia Bratislava Sumbalova Zuzana Slovakia Bratislava Maderova D Slovenia Ljubljana Kopitar-Jerala Natasa Slovenia Ljubljana Pirkmajer Sergej Slovenia Ljubljana Mars T Spain Armilla, Granada Escames Germaine Spain Valencia Fuertes Agudo Marina Spain Sevilla Muntane Jordi Spain Madrid Cano-Abad MF Spain A Coruña Blanco FJ Spain Barcelona Tura Ceide O Spain Madrid Monsalve Maria Spain Sevilla Bustos Matilde Spain Salamanca Almeida Angeles Spain Madrid Lopez MG Spain Barcelona Arago Marc Spain Barcelona Hyrossova Petra Spain Barcelona Smolders V Spain Armilla (Granada) Fernandez-Ortiz Marisol Spain Madrid Cadenas S Spain Barcelona Gil J Spain Valencia Victor Victor Manuel Spain Ourense Reboredo-Rodriguez Patricia Spain Barcelona Gonzalez Casacuberta I Spain Valencia Casado Pinna Marta Spain Lleida Gonzalo Hugo Spain Granada Quiles JL Spain Barcelona Juarez D Spain Lleida Nogueras L Spain Barcelona Zorzano Antonio Spain A Coruña Mayan MD Spain Valencia Borras Consuelo Spain Barcelona Dahdah Norma Ramon Spain Madrid De los Rios C Spain Barcelona Gama Perez Pau Sweden Lund Chamkha Imen Sweden Södertälje Alfaro-Moreno E Sweden Lund Westerlund E Sweden Lund Karlsson M Sweden Lund Simon Serrano Sonia Sweden Linköping Fontes-Oliveira CC Sweden Lund Elmer Eskil Sweden Lund Leffler M Sweden Lund Abliva Sweden, SE Lund Aasander Frostner Eleonor Switzerland Lausanne Zanou Nadege Switzerland Geneva Martinou JC The Netherlands BT Amsterdam Wuest Rob CI Turkey Ankara Engin Ayse Basak Turkey Ankara Tuncay Erkan Turkey Istanbul Baykal AT Turkey Tekirdag Aral Cenk Turkey Izmir Aydin HH USA Philadelphia Starr Jonathan USA St Louis Welch R USA Philadelphia Janowska Joanna USA Fayetteville Rao RR USA Philadelphia Kilbaugh Todd USA Iowa City Buettner Garry R USA Baton Rouge Irving Brian A USA Orlando Coen Paul M USA Fayetteville Iyer Shilpa United Kingdom Oxford Makrecka-Kuka Marina United Kingdom Brighton Moore Anthony L United Kingdom Leicester Moisoi Nicoleta United Kingdom Birmingham Thakker A United Kingdom Birmingham Joanisse S United Kingdom Stoke-on-Trent McStay Gavin P Uruguay Montevideo Radi R
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
