Description
Noncoupled respiration is distinguished from general (pharmacological or mechanical) uncoupled respiration, to give a label to an effort to reach the state of maximum uncoupler-activated respiration without inhibiting respiration. Noncoupled respiration, therefore, yields an estimate of ET-capacity. Experimentally uncoupled respiration may fail to yield an estimate of ET-capacity, due to inhibition of respiration above optimum uncoupler concentrations or insufficient stimulation by sub-optimal uncoupler concentrations. Optimum uncoupler concentrations for evaluation of (noncoupled) ET-capacity require inhibitor titrations (Steinlechner-Maran 1996 Am J Physiol Cell Physiol; Huetter 2004 Biochem J; Gnaiger 2008 POS).
Noncoupled respiration is maximum electron flow in an open-transmembrane proton circuit mode of operation (see ET-capacity).
Abbreviation: E
Reference: Gnaiger 2014 MitoPathways, MitoPedia: Respiratory states
Contributed by Gnaiger E 2010-08-15, edited 2015-01-19.
Is respiration uncoupled - noncoupled - dyscoupled?
- Β» More details: Uncoupler
Biochemical coupling efficiency: from 0 to <1
- Β» More details: OXPHOS coupling efficiency and ET-coupling efficiency
Related MitoPedia pages
- Electron transfer-pathway, ET-pathway
- Electron transfer-pathway states
- Coupling control state E
- Bioblast links: Uncoupling - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- Β» Artefacts by single dose uncoupling
- Β» ATP synthase
- Β» CCCP
- Β» Coupling-control protocol
- Β» DNP
- Β» Dyscoupled respiration
- Β» FCCP
- Β» Is respiration uncoupled - noncoupled - dyscoupled?
- Β» Noncoupled respiration: Discussion
- Β» Uncoupler
- Β» Uncoupled respiration - see Β» Noncoupled respiration
- Β» Uncoupling proteins
- Β» Uncoupling protein 1
- Β» Uncoupler titrations - Optimum uncoupler concentration
- Specific
- Respiratory states and control ratios
- Β» Biochemical coupling efficiency
- Β» Coupling-control state
- Β» Electron-transfer-pathway state
- Β» Electron-transfer pathway
- ET capacity
- Β» E-L coupling efficiency
- Β» Flux control efficiency
- Β» Flux control ratio
- Β» LEAK-control ratio
- Β» LEAK respiration
- Β» Noncoupled respiration
- Β» OXPHOS
- Β» OXPHOS capacity; Β» State 3
- Β» OXPHOS-control ratio, P/E ratio
- Β» Respiratory acceptor control ratio
- Β» ROUTINE-control ratio
- Β» ROUTINE respiration
- Β» ROUTINE state
- Β» State 3u
- Β» State 4
- Β» Uncoupling-control ratio UCR
- Respiratory states and control ratios
- Gnaiger E et al β MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
- General (alphabetical order)
- Other keyword lists
MitoPedia concepts:
Respiratory state,
Recommended
MitoPedia methods:
Respirometry
MitoPedia topics:
EAGLE