O2k-Fluo LED2-Module

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O2k-Fluo LED2-Module

O2k-Catalogue» OROBOROS O2k-Catalogue, Order @OROBOROS

Description The O2k-Fluo LED2-Module is a component of the O2k-Fluorometer. It is an amperometric add-on module to the O2k-Core (Series D upwards), adding a new dimension to HRR. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex red), ATP production (Magnesium green), mt-membrane potential (Safranin, TMRM), Ca2+ (Calcium green), and numerous other applications open for O2k-user innovation.

The O2k-Fluo LED2-Module consists of optical sensors for both O2k-Chambers (LEDs for green and blue excitation), optical filters, Fluorescence-Control Unit for regulation of light intensity, data input into the O2k-Main Unit, and the updated DatLab software.

Product ID 12100-01
Type O2k, O2k-Module, O2k-Fluorometer, MultiSensor, Catalogue
Link O2k-Fluorometer, Oxygraph-2k, MiPNet17.05 O2k-Fluo LED2-Module
Image
Fluorescence-Control Unit lettered.jpg


MitoPedia O2k and high-resolution respirometry: O2k hardware 

The O2k-Fluo LED2-Module consists of

TitleDescriptionProduct idProduct image
O2k-FluorometerO2k-Fluorometer (OROBOROS O2k-Fluorometer, OROBOROS Oxygraph-2k Fluorometer) - the experimental system complete for high-resolution respirometry (HRR) combined with fluorometry. The O2k-Fluorometer includes the O2k-Core, O2k-Fluo LED2-Module and TIP2k, and supports all other add-on O2k-Modules of the Oxygraph-2k. The O2k is a sole source apparatus with no other instruments meeting its specifications.10010-01
O2k-Fluorometer
Power O2k-FluorometerPower O2k-Fluorometer (OROBOROS Power O2k-Fluorometer) - Optional configuration as additional system for increasing output in high-resolution respirometry (HRR) combined with fluorometry. The Power O2k-Fluorometer includes the Power-O2k Core-Unit, O2k-Fluo LED2-Module and TIP2k, and supports all other add-on O2k-Modules of the Oxygraph-2k. It can be added to an existing O2k-Core or O2k-Fluorometer. This application does not require an additional ISS-Integrated Suction System, an O2k-Titration Set and the O2k-Manual. Furthermore, the following OroboPOS Service Tools can be used from the available O2k-Core and are not included: OroboPOS-Mounting Tool, OroboPOS-Polishing Cloth, OroboPOS-Polishing Powder and Pen-Contact Oil. The O2k is a sole source apparatus with no other instruments meeting its specifications.10011-01
O2k-Fluorometer
Stopper\black PEEK\conical Shaft\central PortStopper\black PEEK\conical Shaft\central Port: with conical shaft and one central capillary (with PTFE, graphite, carbon fiber), Volume-Calibration Ring (A or B) for volume adjustment 1.5 to 3.2 ml; 2 mounted O-rings (O-ring\Viton\12x1 mm). The black PEEK stoppers are required for optical O2k-MultiSensor Modules (O2k-Fluo_LED2-Module). Two units of this item are standard components of the O2k-Assembly Kit (O2k-Core).24000-01
Stopper black PEEK conical Shaft central Port.JPG
Cover-Slip\blackA Cover-Slip should be placed on top of the O2k-Stopper to minimize contamination and evaporation of liquid extruding from the capillary of the stopper. The Cover-Slips do not exert any direct effect on oxygen backdiffusion into the O2k-Chamber. Use the the Cover-Slip\black to avoid light penetration and disturbance of fluorescence signals and generally for optical measurements in the Oxygraph-2k. Two units of this item are standard components of the O2k-Assembly Kit (O2k-Core).24411-01
Cover-Slip black.JPG
Fluorescence-Service BoxFluorescence-Service Box44001-01
Fluorescence-Service Box.JPG
Fluorescence-Control UnitFluorescence-Control Unit with O2k-Front Fixation, Current-Control (O2k-Chamber A and B) for regulation of light intensity of the LED in the fluorescence sensors. This item is a standard component of the O2k-Fluorescence LED2-Module.44100-01
Fluorescence-Control Unit lettered.jpg
"display:none"Fluorescence-Sensor: LED with specified wavelength, photodiode, Filter-Cap attached with specific optical filter for the LED and/or photodiode. See Fluorescence-Sensor Green and Fluorescence-Sensor Blue.442##
Fluorescence-Sensor.jpg
Fluorescence-Sensor GreenFluorescence-Sensor Green: excitation LED 525 nm, photodiode, Filter-Cap equipped with Filter Set AmR for Amplex UltraRed measurements when delivered. Two units of this item are standard components of the O2k-Fluo LED2-Module.44210-01
Fluorescence-Sensor Green.JPG
Fluorescence-Sensor BlueFluorescence-Sensor Blue: excitation LED 465 nm, photodiode, Filter-Cap equipped with Filter Set Saf for measurement of mitochondrial membrane potential with Safranin when delivered. Filter sets for Magnesium green® / Calcium green® measurements are included. Two units of this item are standard components of the O2k-Fluo LED2-Module.44220-01
Fluorescence-Sensor Green.JPG
Filter-CapFilter-Cap: Each Fluorescence-Sensor Green and Fluorescence-Sensor Blue is equipped with a removable Filter-Cap for exchange of optical filters for the optical pathways from the LED to the sample and from the sample to the photodiode.44310-01
Filter-Cap.JPG
Filter Set AmRFilter Set AmR: Set of filters for the determination of H2O2 production with Amplex® UltraRed. These filters should be used together with Fluorescence-Sensor Green. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One pair of these filters is attached to Fluorescence-Sensor Green when delivered as part of the O2k-Fluorescence LED2-Module. One filter set is a standard component of the O2k-Fluorescence LED2-Module.44321-01
Filter Set AmR.JPG
Filter Set SafFilter set Saf: Set of filters for the (qualitative) determination of mitochondrial membrane potential with Safranin. These filters should be used together with Fluorescence-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One pair of these filters is attached to Fluorescence-Sensor Blue when delivered as part of the O2k-Fluorescence LED2-Module.


One filter set is a standard component of the O2k-Fluorescence LED2-Module.
44322-01
Filter Set Saf.JPG
Filter Set MgG / CaGFilter set MgG / CaG: Set of filters for the determination of concentraions of Mg2+ or Ca2+ with the fluorophores Magnesium green and Calcium green, respectively. These filters should be used together with Fluorescence-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular). One filter set is a standard component of the O2k-Fluorescence LED2-Module.44323-01
Filter Set MgG CaG.JPG

O2k-Fluo-Manual and O2k-Protocols

» O2k-Fluo Manual
» O2k-Demo experiments
» MitoPedia: Fluorometry


O2k signal and output

  1. O2k signal: The O2k-Fluo LED2-Module is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [Amp]) as the primary signal.
  2. O2k output: type A, B, or C, or combinations.


Scope of applications

The scope of applications is defined by the excitation wavelengths of the Fluorescence-Sensor Green and Fluorescence-Sensor Blue and the selection of emission filters.
The O2k-Fluo LED2-Module is designed for measurements performed on samples placed in a stirred and temperature-controlled chamber. Fluorometric protocols based on measurements in cuvettes of various fluorometers can be used with the O2k-Fluo LED2-Module, and extended by simultaneous measurement of respiration and monitoring or regulation of oxygen concentration. The excitation and emission wavelengths of the fluorophore must be suitable for use with the O2k-Fluo LED2-Module. For examples see LED-intensity and amplification.
The O2k-Fluo LED2-Module is not designed to fulfill the functions of fluorescence microscopes or flow cytometers.


O2k-Fluorometry Workshops


References

O2k-Publications in the MiPMap

»O2k-Publications: Topics

Sort in ascending/descending order by a click on one of the small symbols in squares below.
Default sorting: chronological. Empty fields appear first in ascending order. 
 Was published in yearHas titleOrganismTissue;cell
Liepinsh 2016 Biochem J2016Liepinsh E, Makrecka-Kuka M, Volska K, Kuka J, Makarova E, Antone U, Sevostjanovs E, Vilskersts R, Strods A, Tars K, Dambrova M (2016) Long-chain acylcarnitines determine ischaemia/reperfusion-induced damage in heart mitochondria. Biochem J 473:1191-202.RatHeart
Bouitbir 2016 Antioxid Redox Signal2016Bouitbir J, Singh F, Charles AL, Schlagowski AI, Bonifacio A, Echaniz-Laguna A, Geny B, Krähenbühl S, Zoll J (2016) Statins trigger mitochondrial ROS-induced apoptosis in glycolytic skeletal muscle. Antioxid Redox Signal 24:84-98.RatSkeletal muscle
Other cell lines
Mezera 2016 Oxid Med Cell Longev2016Mezera V, Endlicher R, Kucera O, Sobotka O, Drahota Z, Cervinkova Z (2016) Effects of epigallocatechin gallate on tert-butyl hydroperoxide-induced mitochondrial dysfunction in rat liver mitochondria and hepatocytes. Oxid Med Cell Longev 7573131:8pp.RatLiver
Karlsson 2016 Shock2016Karlsson M, Hara N, Morata S, Sjövall F, Kilbaugh T, Hansson MJ, Uchino H, Elmér E (2016) Diverse and tissue-specific mitochondrial respiratory response in a mouse model of sepsis-induced multiple organ failure. Shock 45:404-10.MouseNervous system
Liver
Nemeth 2016 FASEB J2016Németh B, Doczi J, Csete D, Kacso G, Ravasz D, Adams D, Kiss G, Nagy AM, Horvath G, Tretter L, Mócsai A, Csépányi-Kömi R, Iordanov I, Adam-Vizi V, Chinopoulos C (2016) Abolition of mitochondrial substrate-level phosphorylation by itaconic acid produced by LPS-induced Irg1 expression in cells of murine macrophage lineage. FASEB J 30:286-300.MouseLiver
Timpani 2016 Neurotherapeutics2016Timpani CA, Trewin AJ, Stojanovska V, Robinson A, Goodman CA, Nurgali K, Betik AC, Stepto N, Hayes A, McConell GK, Rybalka E (2016) Attempting to compensate for reduced neuronal nitric oxide synthase protein with nitrate supplementation cannot overcome metabolic dysfunction but rather has detrimental effects in Dystrophin-deficient mdx muscle. Neurotherapeutics [Epub ahead of print].MouseSkeletal muscle
Salin 2016 Physiol Rep2016Salin K, Villasevil EM, Auer SK, Anderson GJ, Selman C, Metcalfe NB, Chinopoulos C (2016) Simultaneous measurement of mitochondrial respiration and ATP production in tissue homogenates and calculation of effective P/O ratios. Physiol Rep 10.14814/phy2.13007.FishesLiver
Abdel-Rahman 2016 Oxid Med Cell Longev2016Abdel-Rahman EA, Mokhtar A, Aaliya A, Radwan Y, Yasseen B, Al-Okda A, Atwa A, Elhanafy E, Habashy M, Ali SS (2016) Resolving contributions of oxygen-consuming and ROS-generating enzymes at the synapse. Oxid Med Cell Longev p19.MouseNervous system
Larsen 2016 FASEB J2016Larsen FJ, Schiffer TA, Ørtenblad N, Zinner C, Morales-Alamo D, Willis SJ, Calbet JA, Holmberg HC, Boushel R (2016) High-intensity sprint training inhibits mitochondrial respiration through aconitase inactivation. FASEB J 30:417-27.HumanSkeletal muscle
Du 2016 J Exp Biol2016Du SN, Mahalingam S, Borowiec BG, Scott GR (2016) Mitochondrial physiology and reactive oxygen species production are altered by hypoxia acclimation in killifish (Fundulus heteroclitus). J Exp Biol 219:1130-8.FishesLiver
O2k versus multiwell respirometer2015-10-05
Logo OROBOROS INSTRUMENTS.jpg
O2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis.
HumanFibroblast
MiPNet18.10 O2k versus multiwell2015-10-05
OROBOROS
O2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis.
HumanFibroblast
MiPNet18.05 Amplex-Mouse-heart2015-06-16
O2k-Protocols
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
MouseHeart
MiPNet20.13 Safranin mt-membranepotential2015-06-15
O2k-Protocols
O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin.
MouseNervous system
MiPNet17.05 O2k-Fluo LED2-Module2015-06-15
O2k-Manual
O2k-Fluo LED2-Module.
MiPNet20.14 AmplexRed H2O2-production2015-06-15
O2k-Protocols
O2k-Fluorometry: HRR and simultaneous determination of H2O2 production with Amplex Red.
MouseHeart
MiPNet18.06 Amplex-Yeast2015-06-15
O2k-Protocols
O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast.
Saccharomyces cerevisiae
Fungi
Roy Chowdhury 2015 Biosci Rep2015Roy Chowdhury SK, Djordjevic J, Albensi B, Fernyhough P (2015) Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep 36.MouseNervous system
Du 2015 J Biol Chem2015Du J, Rountree A, Cleghorn WM, Contreras L, Lindsay KJ, Sadilek M, Gu H, Djukovic D, Raftery D, Satrustegui J, Kanow M, Chan L, Tsang SH, Sweet IR, Hurley JB (2015) Phototransduction influences metabolic flux and nucleotide metabolism in mouse retina. J Biol Chem 291:4698-710.MouseNervous system
Islet cell;pancreas;thymus
Makrecka-Kuka 2015 Biomolecules2015
O2k-Protocols contents
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.
Human
Mouse
Heart
Nervous system
HEK
Krumschnabel 2015 Methods Mol Biol2015
O2k-Protocols contents
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.
MouseNervous system
Kluckova 2015 Cell Death Dis2015Kluckova K, Sticha M, Cerny J, Mracek T, Dong L, Drahota Z, Gottlieb E, Neuzil J, Rohlena J (2015) Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation. Cell Death Dis 6:e1749.Other mammalsLung;gill
Other cell lines
Fibroblast
Chinopoulos 2014 Methods Enzymol2014Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48.HumanHEK
Gnaiger 2014 MitoPathways2014
O2k-Protocols
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp.
Human
Mouse
Heart
Skeletal muscle
Fibroblast
Pham 2014 Am J Physiol2014Pham T, Loiselle D, Power A, Hickey AJ (2014) Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am J Physiol 307:C499–507.RatHeart
Elustondo 2014 Biochim Biophys Acta2014Elustondo PA, Negoda A, Kane CL, Kane DA, Pavlov EV (2014) Spermine selectively inhibits high-conductance, but not low-conductance calcium-induced permeability transition pore. Biochim Biophys Acta 1847:231–40.RatLiver
Iftikar 2014 Thesis University of Auckland2014Iftikar FI (2014) Testing the role of heart mitochondrial stability and function in heart failure of ectotherms exposed to heat stress. Thesis University of Auckland:167pp.FishesHeart
Hunter 2014 PhD Thesis2014Hunter FW (2014) Old target; new paradigm: Determinants of sensitivity to hypoxia-directed anticancer prodrugs. PhD Thesis:1-353.Other mammalsCHO
Soltysinska 2014 PLoS One2014Soltysinska E, Bentzen BH, Barthmes M, Hattel H, Thrush AB, Harper ME, Qvortrup K, Larsen FJ, Schiffer TA, Losa-Reyna J, Straubinger J, Kniess A, Thomsen MB, Brüggemann A, Fenske S, Biel M, Ruth P, Wahl-Schott C, Boushel RC, Olesen SP, Lukowski R (2014) KCNMA1 encoded cardiac BK channels afford protection against ischemia-reperfusion injury. PLoS One 9:e103402.MouseHeart
Skeletal muscle
Kiss 2014 FASEB J2014Kiss G, Konrad C, Pour-Ghaz I, Mansour JJ, Németh B, Starkov AA, Adam-Vizi V, Chinopoulos C (2014) Diaphorases provide NAD+ in anoxia. FASEB J 28:1682-97.MouseLiver
Krumschnabel 2014 Methods Enzymol2014
O2k-Protocols contents
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.
MouseNervous system
Jelenik 2013 Eur Heart J2013Jelenik T, Floegel U, Phielix E, Kaul K, Nowotny P, Partke HP, Schrader J, Roden M, Szendroedi S (2013) Non-alcoholic fatty liver disease and insulin resistance are associated with increased cardiac oxidative stress in mice. Eur Heart J 34:P5045.MouseHeart
Iftikar 2013 PLoS One2013Iftikar FI, Hickey AJ (2013) Do mitochondria limit hot fish hearts? Understanding the role of mitochondrial function with heat stress in Notolabrus celidotus. PLoS One 8:e64120.FishesHeart
Reilly 2013 J Exp Biol2013Reilly BD, Hickey AJ, Cramp RL, Franklin CE (2013) Decreased hydrogen peroxide production and mitochondrial respiration in skeletal muscle but not cardiac muscle of the green-striped burrowing frog, a natural model of muscle disuse. J Exp Biol 217:1087-93.AmphibiansHeart
Skeletal muscle
Hara 2013 Eur J Anaesthesiol2013Hara N, Karlsson M, Sjövall F, Hansson Magnus J, Elmér E, Uchino H (2013) Early brain mitochondrial dysfunction in a mouse model of sepsis: 7AP4‐9. Eur J Anaesthesiol 30,112-112.MouseNervous system
Magnesium Green2013Chinopoulos C (2013) Use of Magnesium Green 5N hexapotassium salt to measure ADP-ATP exchange rates. Mitochondr Physiol Network 2013-02-14.
Gnaiger 2012 Mitochondr Physiol Network Bioblast 20122012
OROBOROS
Gnaiger E, Meissner B, Laner V, eds (2012) Bioblast 2012: Mitochondrial Competence. Mitochondr Physiol Network 17.12. OROBOROS MiPNet Publications, Innsbruck:96 pp. ISBN 978-3-9502399-5-9
Hickey 2012 J Comp Physiol B2012Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182:91-100.FishesHeart
Hunter 2012 Biochem Pharmacol2012Hunter FW, Wang J, Patel R, Hsu HL, Hickey AJ, Hay MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83:574–85.CHO
O2k-Publications in the MiPMap

O2k-Publications: O2k-Fluorometry - Abstracts

Sort in ascending/descending order by a click on one of the small symbols in squares below.
 Has titleOrganismTissue;cell
Krumschnabel 2016b Abstract MitoFit Science Camp 2016O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry.HumanKidney
HEK
Gnaiger 2016 Abstract SFRR Australasia 2016Facts and artefacts in measurements of H2O2 production under hypoxia.Saccharomyces cerevisiae
Nielsen 2016 Abstract IOC116Nielsen B, Woertwein G, Cejvanovic V, Hansen AR, Weimann A, Bjerring PN, Poulsen HE, Joergensen MB (2016) The function of mitochondria and oxidative stress after antidepressants treatment in rats. Mitochondr Physiol Network 21.11RatNervous system
Komlodi 2016 Abstract IOC116Komlodi T, Geibl F, Sassani M, Adam-Vizi V, Tretter L (2016) The effect of ΔΨmt and ΔpH on mitochondrial reactive oxygen species production - A comparative study using mitochondria isolated from different mammalian tissues. Mitochondr Physiol Network 21.11Guinea pigHeart
Skeletal muscle
Nervous system
Sumbalova 2016 Abstract Mito Xmas Meeting InnsbruckMethods for evaluation of mitochondrial membrane potential with multi-sensor high-resolution respirometry: potentiometry vs fluorometry.MouseNervous system
Chicco 2016a Abstract MitoFit Science Camp 2016Oxygen dependence of H2O2 release from skeletal muscle and cardiac mitochondria: influence of sample preparation.MouseHeart
Skeletal muscle
Doerrier 2016b Abstract Mito Xmas Meeting InnsbruckSearching for specific targets of ischemic damage of cardiac mitochondria using O2k-Fluorometry.MouseHeart
Ali 2015 Abstract MiP2015Mitochondrial function and ROS homeostasis in young mouse heart and brain: the sex factor!MouseHeart
Nervous system
Bezuidenhout 2015 Abstract MiPschool Cape Town 2015A study of the effects of fructose feeding & exercise on SIRT 3 regulation in skeletal muscle.Rat
Rohlena 2015 Abstract MiP2015Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation.HumanOther cell lines
Madlala 2015 Abstract MiPschool Cape Town 2015Fructose-induced ROS production: The role of uric acid and transforming growth factor (TGF)-β1.HumanLiver
Other cell lines
Doerrier 2015 Abstract MiP2015Simultaneous measurement of mitochondrial respiration, hydrogen peroxide production, and NADH autofluorescence to assess mitochondrial function.MouseHeart
Hoppel 2015 Abstract MiPschool London 2015Human blood cell types as study models of mitochondrial respiration and H2O2 production.HumanBlood cells
Smenes 2015 Abstract IOC100Smenes BT, Baekkerud FH, Hassel E, Wohlwend M, Slagsvold KH, Rognmo O, Wahba A (2015) Mitochondrial function in patients exercising prior to coronary surgery – A single blinded randomized controlled trial. Mitochondr Physiol Network 20.01.HumanSkeletal muscle
Sumbalova 2015 Abstract MiP2015High-resolution measurement of mitochondrial membrane potential and respiration – comparison of potentiometric and fluorometric methods.MouseNervous system
Doerrier 2015 Abstract MiPschool Greenville 2015"Succinate paradox" study by high resolution respirometry combined with fluorometry and spectrofluorometry: NextGen-O2k.MouseHeart
Abdel-Rahman 2015 Abstract MiPschool Cape Town 2015Assessment of NADPH-dependent oxygen consumption and hydrogen peroxide production by high-resolution respirometry and EPR spectroscopy.Human
Mouse
Nervous system
Gnaiger 2015 Abstract MiP2015Mitochondrial function of cyropreserved HEK 293T cells: development of a reference sample for high-resolution respirometry.HumanHEK
Laner 2015 Abstract MiPschool Cape Town 2015The OROBOROS Oxygraph-2k for high-resolution respirometry and O2k-Fluorometry: a new quality in OXPHOS analysis.Mouse
Saccharomyces cerevisiae
Heart
Meszaros 2014 Abstract MiP2014Methodological aspects of assessing mitochondrial function in small intestinal mucosal samples of rats and guinea pigs.Rat
Guinea pig
Hickey 2014 Abstract MiP2014Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart.RatHeart
Perry 2014 Abstract MiP2014Evaluation of critical experimental parameters for assessing mitochondrial bioenergetics in permeabilized myofibres.HumanSkeletal muscle
Kluckova 2014 Abstract MiP2014Proximal ubiquinone binding site in mitochondrial respiratory Complex II is a target for vitamin E analog MitoVES, an experimental anti-cancer agent. Mitochondr Physiol Network 19.13.Other cell lines
Power 2014 Abstract MiP2014The hot heart: cardiac mitochondrial energetics during hyperthermia.RatHeart
Komlodi 2014 Abstract MiP2014Methylenblue, an enhancer of mitochondrial substrate-level phosphorylation.Guinea pigNervous system
Krumschnabel 2014 Abstract MiP2014Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production.MouseNervous system
Hickey 2013 Abstract MiP2013Hickey AJ, Iftikar FI, MacDonald J, Baker D (2013) Mitochondria in a changing climate? The role of mitochondrial in hyperthermic heart failure in different fish species. Mitochondr Physiol Network 18.08.Fishes
Other mammals
Heart
Jezek 2013 Abstract MiP2013Ježek P, Ježek J, Zelenka J, Dlasková A, Jabůrek M (2013) Antioxidant synergy of mitochondrial uncoupling protein UCP2 and phospholipase iPLA2γ. Mitochondr Physiol Network 18.08.MouseLung;gill
Islet cell;pancreas;thymus
Kressig 2013 Abstract IOC79Kressig F (2013) et al. Mechanism of increased pulmonary vascular remodeling in UCP2 k/o mice. Mitochondr Physiol Network 18.07MouseLung;gill
Other cell lines
Krumschnabel 2013 Abstract MiP2013Krumschnabel G, Eigentler A, Fontana-Ayoub M, Draxl A, Fasching M, Gnaiger E (2013) Tissue homogenates for respiratory OXPHOS analysis in comparative mitochondrial physiology: mouse and trout – heart and liver. Mitochondr Physiol Network 18.08.Mouse
Fishes
Heart
Nervous system
Liver
Laner 2013 ASMRMLaner V, Gradl P, Eigentler A, Fontana-Ayoub M, Fasching M, Gnaiger E (2013) The OROBOROS Oxygraph-2k for high-resolution respirometry and O2k-Fluorometry: a new quality in OXPHOS analysis. ASMRM2013.MouseHeart
Fasching 2012 Abstract BioblastFasching M, Sumbalova Z, Gnaiger E (2012) O2k-Fluorometry - a MitoCom project. Mitochondr Physiol Network 17.12.MouseNervous system
Kiss 2012 Abstract BioblastKiss G, Konrad C, Doczi J, Starkov AA, Kawamata H, Manfredi G, Zhang SF, Gibson GE, Beal MF, Adam-Vizi V, Chinopoulos C (2012) The negative impact of alpha-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation. Mitochondr Physiol Network 17.12.MouseNervous system
Liver
Konrad 2012 Abstract BioblastKonrad C, Kiss G, Torocsik B, Adam-Vizi V, Chinopoulos C (2012) Absence of Ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in C. crangon and P. serratus. Mitochondr Physiol Network 17.12.Artemia
Crustaceans
Horvath 2012 Abstract BioblastHorvath G, Eigentler A, Gnaiger E, Tretter L (2012) Respiratory substrate effects on mitochondrial membrane potential - experiments with mouse cardiac muscle homogenate. Mitochondr Physiol Network 17.12.MouseHeart
Stoeger 2012 Abstract BioblastStoeger H, Fontana-Ayoub M, Eigentler A, Gnaiger E (2012) Cell respiration with yeast as a model. Mitochondr Physiol Network 17.12.Saccharomyces cerevisiae
Fungi
Cervinkova 2012 Abstract BioblastCervinkova Z, Stankova P, Cervinka M, Kucera O, Drahota Z (2012) Evaluation of mitochondrial functions – an important tool for experimental hepatology. Mitochondr Physiol Network 17.12.RatLiver
Sumbalova 2011 Abstract KagoshimaSumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H2O2 production, and the upper limit of mitochondrial performance. Abstract Kagoshima.MouseNervous system
Fasching 2011 Abstract BerlinFasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin.MouseNervous system


Next step

  • NextGen-O2k - the NextGeneration OROBOROS Oxygraph-2k.


MitoPedia methods: Fluorometry