Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(02):1-6. »Versions
The O2k-Fluorescence LED2-Module is an extension of the OROBOROS O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ETS-competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
Labels: MiParea: Respiration Mammal;model: Mouse Tissue;cell: Heart Preparation: Homogenate Regulation: Cyt c Coupling state: LEAK, OXPHOS, ETS Substrate state: CI, CII, CI&II HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol Additional: O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder