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O2k-Catalogue» OROBOROS O2k-Catalogue, Order @OROBOROS

Description OROBOROS O2k-Core - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Core includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Core supports all add-on O2k-Modules of the O2k, and is a component of the O2k-Fluorometer.

On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software.

The O2k is a sole source apparatus with no other instruments meeting its specifications.

Product ID 10000-02
Type O2k, O2k-Module, O2k-Fluorometer, Catalogue
Link O2k, @OROBOROS, Gnaiger_2008_POS
  • A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.

The O2k-Core consists of

TitleDescriptionProduct idProduct image
O2k-Main UnitThe O2k-Main Unit is a component of the O2k-Core. The O2k-Main Unit consists of functionally defined, integral elements, the (O2k-Main Basic, O2k-Peltier Temperature Control, two O2k-Electromagnetic Stirrer Twin-Control units, two O2k-Amperometric OroboPOS Twin-Channels, O2k-Barometric Pressure Transducer), which cannot be obtained separately.20000
O2k-Assembly KitThe O2k-Assembly Kit is a component of the O2k-Core, consisting of 2 O2k-Chambers (Duran glass), 2 PVDF Stirrer-Bars, 2 PVDF or PEEK O2k-Stoppers, O2k-Chamber Holders OroboPOS-Holders and OroboPOS-Connectors, and cables (power supply, USB-connection). Several components of the O2k-Assembly Kit are included in the O2k-Service Box either for shipment or for storage.20100
2 OroboPOSThe 2 OroboPOS set (two polarographic oxygen sensors, POS) is included in the O2k-Core, shipped with the O2k in the O2k-Service Box. For details, see OroboPOS.20600
OroboPOS-Service KitOroboPOS-Service_Kit, with O2k-Service Box including all oxygen sensor service accessories for membrane mounting and service of the OroboPOS.20610-02
ServiceBox G 2.jpg
DatLabDatLab is the O2k-Software for Data Acquisition & Analysis, specifically developed for O2k high-resolution respirometry with the Oxygraph-2k. The newest DatLab version is included in the O2k-Core, and updates of DatLab are free of charge during the warranty period of the O2k.20700
ISS-Integrated Suction SystemISS-Integrated Suction System: Suction pump 230 or 120 V with stainless steel housing, 2 liter waste bottle, filter and tubing; for siphoning off excess medium from the O2k-Stopper and for emptying the O2k-Chambers. The ISS is included as a standard component of the O2k-Core. Media containing living cells or microorganisms, various poisons (inhibitors, uncouplers) and mixtures of proteins and substrates are safely disposed off in the 2-litre waste bottle.

The suction pump (230 V or 120 V) is enclosed in a stainless steel housing, which holds a readily accessible mains switch, two removable receptacles for the tip of the tubing, an easily removable and safe connection to the gas filter which further connects to the fully stabilized waste bottle. The waste bottle with filter can be simply removed for emptying, cleaning and drying.

For specific country configurations, see Product ID 28101, 28102, 28103.
O2k-Titration SetThe O2k-Titration Set consists of Hamilton microsyringes (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 2 x 50 mm3, 1 x 500 mm3), provided in the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks. The O2k-Titration Set is a basic HRR-Accessory included in the O2k-Core.20820-02
O2k-Titration Set.JPG
O2k-CompendiumO2k-Compendium for high-resolution respirometry, consisting of the O2k-Manual and a USB flash drive with DatLab, O2k-Manual, O2k-Protocols, O2k-Publications, and info on O2k-Workshops. The O2k-Compendium is a component of the O2k-Core.20900
O2k-Workshop\Registration waivedO2k-Workshop\Registration waived
  • A registration fee is waived in conjunction with the purchase of the O2k-Core (academic discount), valid during the O2k-Warranty Period.
Packing\O2k-Box 1+2Packing\O2k-Box 1+2 for shipping the O2k-Core. O2k-WorldWide delivery, insurance and handling are included in the O2k-Core.99000

O2k-Core manual and selected protocols

» Contents: O2k-Core Manual DatLab 6.pdf
ChapterViewSectionLast update
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.
Gnaiger 2014 MitoPathwaysBioblast pdf
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp.
MiPNet07.08 User informationBioblast pdf
O2k-Manual: user information. PLEASE STUDY THIS MANUAL.
MiPNet08.09 CellRespirationBioblast pdf
O2k-Protocols contents
High-resolution respirometry and coupling control protocol with intact cells: ROUTINE, LEAK, ETS, ROX.
MiPNet10.04 CellRespirationBioblast pdf
An experiment with high-resolution respirometry: coupling control in cell respiration.
MiPNet12.01 Suppl T-issueBioblast pdf
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibres.
MiPNet12.11 MitoRespirationBioblast pdf
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
MiPNet12.17 BovineHeartMitoBioblast pdf
O2k-Protocols contents
Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests.
MiPNet12.23 FibreRespirationBioblast pdf
O2k-Protocols contents
Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle.
MiPNet15.09. Yeast: HRR Reference AssayBioblast pdf
A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast.
MiPNet17.03 Shredder vs FibresBioblast pdf
Mitochondrial respiration in permeabilized fibres versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
MiPNet19.18 O2k-Core manualBioblast pdf
O2k-Core manual contents.
MiPNet19.18A O2k-startBioblast pdf
Oxygraph-2k: start high-resolution respirometry.
MiPNet19.18B POS-serviceBioblast pdf
Service of the polarographic oxygen sensor OroboPOS.
MiPNet19.18C DatLab-guideBioblast pdf
DatLab-guide through the menus.
MiPNet19.18D O2k-calibrationBioblast pdf
O2k-calibration by DatLab.
MiPNet19.18E O2 flux analysisBioblast pdf
Oxygen flux analysis: DatLab real-time.
MiPNet20.04 O2k-checklistBioblast pdf
O2k-checklist: get started with an O2k-experiment.
MiPNet21.16 DatLab 7Bioblast pdf
DatLab 7: innovations.
Pesta 2012 Methods Mol BiolBioblast pdf
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.

MitoPedia O2k and high-resolution respirometry: O2k hardware