O2k-Core

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O2k-Core

O2k-Catalogue» OROBOROS O2k-Catalogue, Order @OROBOROS

Description OROBOROS O2k-Core - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Core includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Core supports all add-on O2k-Modules of the O2k, and is a component of the O2k-Fluorometer.

On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software.

The O2k is a sole source apparatus with no other instruments meeting its specifications.

Product ID 10000-02
Type O2k, O2k-Module, O2k-Fluorometer, Catalogue
Link O2k, @OROBOROS, Gnaiger_2008_POS
Image
O2k-Core-Concept.jpg
  • A PC is required (not included), connecting DatLab to the O2k via a USB port. Minimum requirement: Pentium III, 256 MB, Win 2000™ or Win XP™.


The O2k-Core consists of

Title Description Product id Product image
O2k-Main Unit The O2k-Main Unit is a component of the O2k-Core. The O2k-Main Unit consists of functionally defined, integral elements, the (O2k-Main Basic, O2k-Peltier Temperature Control, two O2k-Electromagnetic Stirrer Twin-Control units, two O2k-Amperometric OroboPOS Twin-Channels, O2k-Barometric Pressure Transducer), which cannot be obtained separately. 20000
O2k-Assembly Kit The O2k-Assembly Kit is a component of the O2k-Core, consisting of 2 O2k-Chambers (Duran glass), 2 PVDF Stirrer-Bars, 2 PVDF or PEEK O2k-Stoppers, O2k-Chamber Holders OroboPOS-Holders and OroboPOS-Connectors, and cables (power supply, USB-connection). Several components of the O2k-Assembly Kit are included in the O2k-Service Box either for shipment or for storage. 20100
2 OroboPOS The 2 OroboPOS set (two polarographic oxygen sensors, POS) is included in the O2k-Core, shipped with the O2k in the O2k-Service Box. For details, see OroboPOS. 20600
OroboPOS.JPG
OroboPOS-Service Kit OroboPOS-Service_Kit, with O2k-Service Box including all oxygen sensor service accessories for membrane mounting and service of the OroboPOS. 20610-02
ServiceBox G 2.jpg
DatLab DatLab is the O2k-Software for Data Acquisition & Analysis, specifically developed for O2k high-resolution respirometry with the Oxygraph-2k. The newest DatLab version is included in the O2k-Core, and updates of DatLab are free of charge during the warranty period of the O2k. 20700
ISS-Integrated Suction System ISS-Integrated Suction System: Suction pump 230 or 120 V with stainless steel housing, 2 liter waste bottle, filter and tubing; for siphoning off excess medium from the O2k-Stopper and for emptying the O2k-Chambers. The ISS is included as a standard component of the O2k-Core. Media containing living cells or microorganisms, various poisons (inhibitors, uncouplers) and mixtures of proteins and substrates are safely disposed off in the 2-litre waste bottle.

The suction pump (230 V or 120 V) is enclosed in a stainless steel housing, which holds a readily accessible mains switch, two removable receptacles for the tip of the tubing, an easily removable and safe connection to the gas filter which further connects to the fully stabilized waste bottle. The waste bottle with filter can be simply removed for emptying, cleaning and drying.

For specific country configurations, see Product ID 28101, 28102, 28103.
20810
ISS.jpg
O2k-Titration Set The O2k-Titration Set consists of Hamilton microsyringes (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 2 x 50 mm3, 1 x 500 mm3), provided in the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks. The O2k-Titration Set is a basic HRR-Accessory included in the O2k-Core. 20820-02
O2k-Titration Set.JPG
O2k-Compendium O2k-Compendium for high-resolution respirometry, consisting of the O2k-Manual and a USB flash drive with DatLab, O2k-Manual, O2k-Protocols, O2k-Publications, and info on O2k-Workshops. The O2k-Compendium is a component of the O2k-Core. 20900
Logo OROBOROS INSTRUMENTS.jpg
O2k-Workshop\Registration waived O2k-Workshop\Registration waived
  • A registration fee is waived in conjunction with the purchase of the O2k-Core (academic discount), valid during the O2k-Warranty Period.
70000
O2k-Workshops.png
Packing\O2k-Box 1+2 Packing\O2k-Box 1+2 for shipping the O2k-Core. O2k-WorldWide delivery, insurance and handling are included in the O2k-Core. 99000


O2k-Core manual and selected protocols

» Contents: O2k-Core Manual DatLab 6.pdf
Chapter View Section Last update
Gnaiger 2008 POS Bioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.
2008
Gnaiger 2014 MitoPathways Bioblast pdf
O2k-Protocols
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp.
2014
MiPNet07.08 User Information Bioblast pdf
O2k-Manual
O2k-Manual: user information. PLEASE STUDY THIS MANUAL.
2015-11
MiPNet08.09 CellRespiration Bioblast pdf
O2k-Protocols contents
High-resolution respirometry and coupling control protocol with intact cells: ROUTINE, LEAK, ETS, ROX.
2016-08-11
MiPNet10.04 CellRespiration Bioblast pdf
O2k-Protocols
An experiment with high-resolution respirometry: coupling control in cell respiration.
2016-08-08
MiPNet12.01 Suppl T-issue Bioblast pdf
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibres.
2014-07-20
MiPNet12.11 MitoRespiration Bioblast pdf
O2k-Protocols
Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria.
2014-02-20
MiPNet12.17 BovineHeartMito Bioblast pdf
O2k-Protocols contents
Mitochondrial respiration with frozen bovine heart mitochondria: diagnostic tests.
2011-12-11
MiPNet12.23 FibreRespiration Bioblast pdf
O2k-Protocols contents
Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle.
2016-07-19
MiPNet15.09. Yeast: HRR Reference Assay Bioblast pdf
O2k-Protocols
A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast.
2012-01-05
MiPNet17.03 Shredder vs Fibres Bioblast pdf
O2k-Protocols
Mitochondrial respiration in permeabilized fibres versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
2015-06-18
MiPNet19.18 O2k-Core manual Bioblast pdf
O2k-Manual
O2k-Core manual contents.
2016-08-08
MiPNet19.18A O2k-start Bioblast pdf
O2k-Manual
Oxygraph-2k: start high-resolution respirometry.
2016-08-08
MiPNet19.18B POS-service Bioblast pdf
O2k-Manual
Service of the polarographic oxygen sensor OroboPOS.
2016-08-08
MiPNet19.18C DatLab-guide Bioblast pdf
O2k-Manual
DatLab-guide through the menus.
2016-08-08
MiPNet19.18D O2k-calibration Bioblast pdf
O2k-Manual
O2k-calibration by DatLab.
2015-08-08
MiPNet19.18E O2-flux analysis Bioblast pdf
O2k-Manual
Oxygen flux analysis: DatLab real-time.
2016-08-08
MiPNet20.04 O2k-checklist Bioblast pdf
O2k-Manual
O2k-checklist: get started with an O2k-experiment.
2016-08-05
MiPNet21.16 DatLab 7 Bioblast pdf
OROBOROS-Info
DatLab 7: innovations.
2016-08-15
Pesta 2012 Methods Mol Biol Bioblast pdf
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.
2012


MitoPedia O2k and high-resolution respirometry: O2k hardware 

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