POS-Holder: Difference between revisions
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{{MitoPedia without banner | {{MitoPedia without banner | ||
|abbr= | |abbr= | ||
|description=[[Image:Pos_holder.JPG|right|180px]]The ''' | |description=[[Image:Pos_holder.JPG|right|180px]]The '''POS-Holder''', made from blue POM, is screwed into the copper block of the [[O2k-Main Unit]], guiding theguiding the [[OroboPOS|POS]] to the 2-mL [[O2k-chamber]], and keeping the SmartPOS/OroboPOS-Connector in a fixed position for sealing the O2k-chamber with the [[POS-Seal Tip]]. In addition, the POS-Holder fixes the O2k-Chamber in an accurate rotational position by pressing against the angular cut of the glass chamber. | ||
Two units of this item are standard components mounted on the [[O2k-Main Unit]]. | Two units of this item are standard components mounted on the [[O2k-Main Unit]]. | ||
|info=[ | |info=[https://www.oroboros.at/index.php/product/orobopos-holder/ '''Product details and purchase information'''] | ||
Β | |||
}} | }} | ||
== Assembly == | |||
::::* Manual: [https://wiki.oroboros.at/images/2/29/MiPNet22.11_O2k-FluoRespirometer-manual.pdf MiPNet22.11_O2k-FluoRespirometer-manual.pdf] Section 5.2. | |||
::::* Video: [https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be Chamber service Insert O2k Chamber] | |||
== A damaged POS-Holder may cause medium to leak from the O2k-Chamber == | |||
:::* The POS-Holder may be damaged: | |||
::::# During chamber assembly, when fixing the chamber in a wrong rotational position with the [[O2k-Chamber Holder]] (from above) before the POS-Holder is fully inserted, and subsequently screwing tightly the POS-Holder (''see center picture below'').Β | |||
::::# After fully unscrewing the POS-Holder and forcing during reassembly an improperly aligned holder into the thread of the [[O2k-Main Unit]]. | |||
::::* We recommend to check before any reassembly the mechanical integrity of the POS-Holder (''see'' pictures below). | |||
[[File:OroboPOS-Holder002.jpg|left|200px]] | |||
[[File:OroboPOS-Holder003.jpg|right|200px]] | |||
[[File:OroboPOS-Holder004.jpg|centre|200px]] | |||
* Left picture: brand new POS-Holder, no damage on the thread, no damage on the edge. | |||
* Center picture: used POS-Holder, almost no damage on the thread, the edge is slightly damaged. | |||
* Right picture: heavily used POS-Holder, the thread is slightly damaged, serious damage on the edge of the aperture. | |||
::::* Replacement of a damaged POS-Holder is recommended. For ordering spare parts: [https://www.oroboros.at/index.php/o2k-products/spares/ Spare parts & consumables] | |||
''See'' [[Talk:POS-Holder|Discussion page]] | |||
== Keywords == | |||
{{Template:Keywords: Chamber volume}} | |||
Latest revision as of 11:13, 27 July 2023
POS-Holder |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
The POS-Holder, made from blue POM, is screwed into the copper block of the O2k-Main Unit, guiding theguiding the POS to the 2-mL O2k-chamber, and keeping the SmartPOS/OroboPOS-Connector in a fixed position for sealing the O2k-chamber with the POS-Seal Tip. In addition, the POS-Holder fixes the O2k-Chamber in an accurate rotational position by pressing against the angular cut of the glass chamber.
Two units of this item are standard components mounted on the O2k-Main Unit.
Reference: Product details and purchase information
Assembly
- Manual: MiPNet22.11_O2k-FluoRespirometer-manual.pdf Section 5.2.
A damaged POS-Holder may cause medium to leak from the O2k-Chamber
- The POS-Holder may be damaged:
- During chamber assembly, when fixing the chamber in a wrong rotational position with the O2k-Chamber Holder (from above) before the POS-Holder is fully inserted, and subsequently screwing tightly the POS-Holder (see center picture below).
- After fully unscrewing the POS-Holder and forcing during reassembly an improperly aligned holder into the thread of the O2k-Main Unit.
- We recommend to check before any reassembly the mechanical integrity of the POS-Holder (see pictures below).
- Left picture: brand new POS-Holder, no damage on the thread, no damage on the edge.
- Center picture: used POS-Holder, almost no damage on the thread, the edge is slightly damaged.
- Right picture: heavily used POS-Holder, the thread is slightly damaged, serious damage on the edge of the aperture.
- Replacement of a damaged POS-Holder is recommended. For ordering spare parts: Spare parts & consumables
See Discussion page
Keywords
- Β»Bioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Β» Volume
- Units: Liter [L]; 1 L = 1 dm3 = 10-3 m3; 1 mL = 1 cm3; 1 Β΅L = 1 mm3
- Β» Volume
- On chamber volume
- Components of the experimental O2k-chambers
- Β» Stirrer-Bar\white PVDF\15x6 mm
- Β» Stirrer-Bar sV\white PVDF\11.5x6.2 mm
- Β» Stopper\black PEEK\conical Shaft\central Port
- Β» Stopper sV\black PEEK\conical Shaft\central Port
- Β» O-ring\Viton\12.5x1 mm
- Β» O-ring sV\Viton\9.5x1 mm
- Β» Volume-Calibration Ring
- Β» Volume-Calibration Ring sV
- Β» Stopper-Spacer
- Β» Cover-Slip\black
- Β» O2k-Chamber Holder
- Β» O2k-Chamber Holder sV
- Β» OroboPOS-Holder
- Β» OroboPOS-Holder sV
- Maintencance
MitoPedia O2k and high-resolution respirometry:
O2k hardware,
O2k-Open Support,
O2k-Respirometry,
O2k-FluoRespirometry