POS-Holder

                  

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POS-Holder

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MitoPedia O2k and high-resolution respirometry: Oroboros Open Support 

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Description

The POS-Holder, made from blue POM, is screwed into the copper block of the Oroboros Main Unit, guiding the POS to the 2-mL Oroboros chamber, and keeping the SmartPOS/OroboPOS-Connector in a fixed position for sealing the Oroboros chamber with the POS-Seal Tip. In addition, the POS-Holder fixes the Oroboros chamber in an accurate rotational position by pressing against the angular cut of the glass chamber.

Two units of this item are standard components mounted on the Oroboros Main Unit.

Reference: Product details and purchase information

Assembly

• Manual: MiPNet22.11_O2k-FluoRespirometer-manual.pdf Section 5.2.

• Video: Chamber service Insert Oroboros chamber

A damaged POS-Holder may cause liquid leak from the Oroboros chamber

• The POS-Holder may be damaged:

1. During chamber assembly, when fixing the chamber in a wrong rotational position with the O2k-Chamber Holder (from above) before the POS-Holder is fully inserted, and subsequently screwing tightly the POS-Holder (see center picture below).
2. After fully unscrewing the POS-Holder and forcing during reassembly an improperly aligned holder into the thread of the Oroboros Main Unit.

• We recommend to check before any reassembly the mechanical integrity of the POS-Holder (see pictures below).

• Left picture: brand new POS-Holder, no damage on the thread, no damage on the edge.
• Center picture: used POS-Holder, almost no damage on the thread, the edge is slightly damaged.
• Right picture: heavily used POS-Holder, the thread is slightly damaged, serious damage on the edge of the aperture.

• Replacement of a damaged POS-Holder is recommended. For ordering spare parts: Spare parts & consumables

See Discussion page

Keywords

»Bioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

» Volume

• Units: Liter [L]; 1 L = 1 dm³ = 10^-3 m³; 1 mL = 1 cm³; 1 µL = 1 mm³

» Oroboros chamber

» The experimental chamber in the instrumental Oroboros Chamber

» O2k-Chamber sV

» Calibration of Oroboros chamber volume

• On chamber volume

» Oroboros chamber

» Chamber volume

» Oroboros paradigm shift

» Cell count and normalization in HRR

» Open chamber

» Closed chamber

• Components of the experimental Oroboros chambers

» Stirrer-Bar\white PVDF\15x6 mm

» Stirrer-Bar sV\white PVDF\11.5x6.2 mm

» Stopper\black PEEK\conical Shaft\central Port

» Stopper sV\black PEEK\conical Shaft\central Port

» O-ring\Viton\12.5x1 mm

» O-ring sV\Viton\9.5x1 mm

» Volume-Calibration Ring

» Volume-Calibration Ring sV

» Stopper-Spacer

» Cover-Slip\black

» O2k-Chamber Holder

» O2k-Chamber Holder sV

» OroboPOS-Holder

» OroboPOS-Holder sV

• Maintencance

» Oroboros chamber cleaning and ISS

» Quality control: Oroboros chamber test

MitoPedia O2k and high-resolution respirometry: Oroboros hardware, Oroboros Open Support, O2k-Respirometry, O2k-FluoRespirometry