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• NADH calibration - DatLab
  |description='''NADH calibration'''
  361 bytes (39 words) - 11:56, 25 March 2022

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• SUIT-032 NADH mt D078
  ...''A''': protocol for simultaneous determination of O₂ flux and NADH autofluorescence in mitochondrial preparations (isolated mitochondria, tiss |application=NADH
  3 KB (442 words) - 10:37, 12 January 2024
• NADH calibration - DatLab
  |description='''NADH calibration'''
  361 bytes (39 words) - 11:56, 25 March 2022
• SUIT-006 NADH mt D084
  ...''A''': protocol for simultaneous determination of O₂ flux and NADH autofluorescence in mitochondrial preparations (isolated mitochondria, tiss |application=NADH
  3 KB (462 words) - 17:23, 21 December 2023
• NextGen-O2k Documentation
  ...n for using the NextGen-O2k. Furthermore, trailers for the new modules (Q, NADH and PB) were recorded and published. ...www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]<br>
  5 KB (760 words) - 13:23, 3 April 2022
• Ghelli 1997 J Biochem
  ...e to the maximal protonmotive force in coupled submitochondrial particles. NADH addition to particles in which ubiquinol oxidation is blocked by inhibitors
  2 KB (254 words) - 16:41, 7 November 2016
• High-resolution respirometry
  ...e [[NextGen-O2k]] all-in-one, however, is unique in supporting Q-Redox and NADH-Redox Modules.
  4 KB (482 words) - 09:09, 2 April 2024
• SUIT-021 Fluo mt D036
  ...hway control state|S-]] pathway in the Q-junction and to investigate the [[NADH Electron transfer-pathway state| N-]] and [[NS-pathway control state| NS-pa ...needs to be done. More information on our USB: Instrumental Protocols/Fluo calibration.
  6 KB (834 words) - 14:07, 6 September 2021
• Hydrogen peroxide
  === H₂O₂ calibration === ::::* NADH-pathway initiated H₂O₂ production
  4 KB (646 words) - 12:06, 9 November 2021
• SUIT-006 Fluo mt D034
  ...ndrial membrane potential]] with fluorescent dyes. In this protocol, the [[NADH Electron transfer-pathway state]] can be analyzed in [[Mitochondrial prepar ...needs to be done. More information on our USB: Instrumental Protocols/Fluo calibration.
  4 KB (623 words) - 14:08, 6 September 2021
• Doerrier 2018 Methods Mol Biol
  ...nsor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background O2 flux (systemic flux compensa ...2021 AmR-O2, MitoFit 2021 AmR, MitoFit 2021 Tissue normoxia, MitoFit 2022 NADH, MitoFit 2021 BCA, MitoFit 2021 PLT, PLoSONE2022ace-sce, MitoFit2022QC
  6 KB (850 words) - 12:27, 5 March 2024
• SUIT-020 Fluo mt D033
  ...needs to be done. More information on our USB: Instrumental Protocols/Fluo calibration. ...pathway control state| NS-]], [[Succinate pathway control state| S]] and [[NADH Electron transfer-pathway state| N-]] pathway control state.
  5 KB (655 words) - 08:41, 30 September 2020
• Meszaros 2018 EBEC2018
  ...> values were calculated using the '''O2kinetics''' software for automatic calibration and correction of O₂ signals, data processing and curve fitting. ...0.07 kPa for NADH-linked LEAK respiration with glutamate&malate (GM), and NADH-&succinate-linked OXPHOS capacity with GM and pyruvate, in agreement with a
  3 KB (461 words) - 19:09, 10 January 2022
• Galluzzi 2014 Methods Enzymol
  ...to membrane potential and oxygen consumption in mitochondria with improved calibration 21. Analysis of hypoxia-induced metabolic reprogramming
  3 KB (326 words) - 11:39, 9 March 2015
• Meszaros 2018 Mt Med Hinxton
  ...-linked LEAK respiration with glutamate&malate, N(GM)''_L'', and NADH-&succinate-linked OXPHOS capacity including GM and pyruvate, NS(GMP)''<sub>
  4 KB (547 words) - 19:03, 10 January 2022
• Safranin
  ...nm and emission wavelength of 587 nm. Safranin is a potent inhibitor of [[NADH electron transfer-pathway state |N-linked respiration]] and of the [[phosph ::::* Final concentration: 0.5-2 µM. Stepwise titration for calibration.
  13 KB (1,842 words) - 17:09, 17 January 2024
• SUIT-009 AmR mt D021
  ...AmplexRed_H2O2-production#Calibration_with_H2O2|H₂O₂ calibration steps]] are done during the experiment. Since the H₂O_{2</sub
  5 KB (681 words) - 00:11, 13 April 2021
• O2k-OpenSupportAlert2020 Archive
  Yes, oxygen signals obtained after proper calibration are independent of the gain setting, and can therefore be grouped together Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By
  27 KB (4,026 words) - 11:53, 29 March 2021
• Chinopoulos 2014 Methods Enzymol
  ...quations. Here, we describe a variant of this method involving an improved calibration step. This step minimizes errors that may be introduced during the conversi |additional=Safranin, MgG, O2k-brief, ATP, MitoFit 2021 MgG, MitoFit 2022 NADH, O2k-Demo, ATP
  3 KB (420 words) - 11:49, 27 October 2023
• Open innovation management
  ...t to the company producing the LED considered most promising for measuring NADH fluorescence. His group is testing the O2k-Fluo Module Module with alternat ...>2}O2. The usual method of opening the chamber, however, disturbs the calibration of the TPP⁺ electrode. Solutions for both problems were initiall
  5 KB (678 words) - 16:43, 28 May 2019
• SUIT-009 AmR ce-pce D019
  ...AmplexRed_H2O2-production#Calibration_with_H2O2|H₂O₂ calibration steps]] are done during the experiment. The experimental control for O<sub>
  5 KB (832 words) - 00:10, 13 April 2021

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