Description
Air calibration of an oxygen sensor (polarographic oxygen sensor) is performed routinely on any day before starting a respirometric experiment. The volume fraction of oxygen in dry air is constant. An aqueous solution in equilibrium with air has the same partial pressure as that in water vapour saturated air. The water vapour is a function of temperature only. The partial oxygen pressure in aqueous solution in equilibrium with air is, therefore, a function of total barometric pressure and temperature. Bubbling an aqueous solution with air generates deviations from barometric pressure within small gas bubbles and is, therefore, not recommended. To equilibrate an aqueous solution ata known partial pressure of oxygen [kPa], the aqueous solution is stirred rigorously in a chamber enclosing air at constant temperature. The concentration of oxygen, cO2 [Β΅M], is obtained at any partial pressure by multiplying the partial pressure by the oxygen solubility, SO2 [Β΅M/kPa]. SO2 is a function of temperature and composition of the salt solution, and is thus a function of the experimental medium. The solubility factor of the medium, FM, expresses the oxygen solubility relative to pure water at any experimental temperature. FM is 0.89 in serum (37 Β°C) and 0.92 in MiR06 or MiR05 (30 Β°C and 37 Β°C).
Abbreviation: R1
Reference: MiPNet06.03 POS-calibration-SOP, Oxygen calibration - DatLab, Oxygen sensor test
- Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- Β» Oxygen signal
- Β» O2k signal line
- Specific
- O2k-Procedures
- MiPNet O2k-Procedures
- General
- Β» Oxygen, dioxygen, O2
- Β» Oxygen calibration - DatLab
- Β» Oxygen solubility
- Β» Oxygen solubility factor
- Β» Oxygen pressure
- Β» Concentration
- Β» Activity
- Β» Pressure - Pascal
- Β» Barometric pressure
- Β» High-resolution respirometry
- Β» OroboPOS
- Β» Polarographic oxygen sensor
- Β» MitoFit Quality Control System
- Β» Sensitivity
- General
- Other keyword lists
MitoPedia methods:
Respirometry
MitoPedia Oroboros and high-resolution respirometry:
DatLab,
Oroboros QM
MitoPedia topics:
Media for respirometry