MiPNet23.01 IOC126 Innsbruck AT: Difference between revisions

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{{OROBOROS header page name}}  
{{OROBOROS header page name}}  
{{Publication
{{Publication
|title= [[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
|title= [[Image:OROBOROS-solo.png|right|60px|link=Oroboros Instruments]]
'''Innsbruck AT''', 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. '''IOC126'''.
'''Innsbruck AT''', 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. '''IOC126'''.
|info=
|info=
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[[File:IOC126.png|center|500px|data analysis]]
[[File:IOC126.png|center|500px|data analysis]]


== Feedback ==
::::4 feedback forms returned
{| class="wikitable"
|-
! Question
! Rating (average)
|-
|In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered).
|4.50
|-
|Balance between practical “hands-on” work and “theoretical” explanations: Please rate from 1 (too much “hands-on”) to 5 (too much theory).
|2.88
|-
|Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content:
|3.50
|-
|Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language:
|4.25
|-
|Level of tutoring during the “hands-on” work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently).
|3.25
|-
|Quality of tutoring: Please rate from 1 (poor) to 5 (excellent).
|4.00
|-
|Intensity of the O2k-Course: Please rate from 1 (I would have liked to do and hear more) to 5 (I felt overwhelmed).
|3.50
|-
|Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent).
|4.00
|}


== Venue ==
== Venue ==
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|-
|-
|09:30
|09:30
|Erich Gnaiger: Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. See: [http://www.mitoeagle.org/index.php/Gnaiger_2017_MiP2017 Abstract].
|Erich Gnaiger: Protonmotive force and respiratory control. [http://www.mitoeagle.org/index.php/MitoEAGLE_preprint_2017-09-21 MitoEAGLE preprint 2017-09-21].
|MiP''Art''
|MiP''Art''
|-
|-
|-
|-
|10:00
|10:00
|O2k-MultiSensor overview, DatLab 7, DatLab 7 Protocols.
|DatLab 7, DL-Protocols.
|MiP''Art''
|MiP''Art''
|-
|-
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|-
|-
|11:00
|11:00
|Determination of mt-membrane potential with potentiometric O2k-TPP+ ISE-Module - an introduction.
|Hands-on: Assembly and maintenance of TPP+ and reference electrodes. [[MiPNet15.03 O2k-MultiSensor-ISE]].
|MiP''Art''
|MitoFit ORO Lab
|-
|-
|11:45
|12:15
|''Lunch break''
|''Lunch break''
|
|
|-
|-
|13:15
|13:30
|Hands-on: Assembly and maintenance of TPP+ and reference electrodes [[MiPNet15.03 O2k-MultiSensor-ISE]].
|Determination of mt-membrane potential with potentiometric O2k-TPP+ ISE-Module - an introduction.
|MitoFit ORO Lab
|MiP''Art''
|-
|-
|14:30
|14:30
|Hands-on: Set up of the instruments with TPP+ and reference electrodes, TPP+ calibration.
|Hands-on: Set up of the instruments with TPP+ and reference electrodes, O2 air calibration and calibration of TPP+ electrode.
|MitoFit ORO Lab  
|MitoFit ORO Lab  
|-
|-
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|-
|-
|17:00
|17:00
|Hands-on continued: TPP+ chemical background experiment [[MiPNet14.05 TPP-mtMembranePotential]].  
|Hands-on continued: TPP+ chemical background experiment. [[MiPNet14.05 TPP-mtMembranePotential]].  
|MitoFit ORO Lab  
|MitoFit ORO Lab  
|}
|}
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|-
|-
|09:00
|09:00
|Introduction in planning and performing a TPP+ experiment.
|Hands-on: O2 air calibration and calibration of TPP+ electrode.
|MitoFit ORO Lab  
|MitoFit ORO Lab  
|-
|-
|09:30
|09:30
|Hands-on: Calibration of TPP+ electrode.
|Introduction in planning and performing a TPP+ experiment.
|MitoFit ORO Lab  
|MitoFit ORO Lab  
|-
|-
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|-
|-
|14:30
|14:30
|Analysis of TPP+ experiment, discussion.
|Analysis of TPP+ experiment. Interdependence between O2 flux and mt-membrane potential.
|MitoFit ORO Lab  
|MitoFit ORO Lab  
|-
|-
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|-
|-
|16:30
|16:30
|Four compartment model - the effect of parameters on calculated mt-membrane potential.
|The four compartment model - the effect of parameters on calculated mt-membrane potential. [http://wiki.oroboros.at/index.php/MiPNet15.03_O2k-MultiSensor-ISE#Calculation_of_mitochondrial_membrane_potential_from_measurement_of_TPP.2B The analysis of the model].
|MiP''Art''
|MiP''Art''
|-
|-
|17:30  
|17:30  
|Erich Gnaiger: Mitochondrial membrane potential.
|Erich Gnaiger: Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. [http://www.mitoeagle.org/index.php/Gnaiger_2017_MiP2017 Abstract].
|MiP''Art''
|MiP''Art''
|-
|-
Line 128: Line 161:
|-
|-
|09:00
|09:00
|Determination of mt-membrane potential with fluorescence method (TMRM, safranin) - O2k FluoRespirometer/O2k-Fluo LED2-Module - an introduction.
|Determination of mt-membrane potential with fluorescence method (TMRM, safranin) - O2k FluoRespirometer - an introduction. [[MiPNet22.11 O2k-FluoRespirometer manual]], [[MiPNet20.13 Safranin mt-membranepotential]].
|MiP''Art''  
|MiP''Art''  
|-
|-
|10:00
|10:00
|Hands-on: O2k setup for fluorescence experiment, choosing fluorescence sensors.
|Hands-on: O2 air calibration and O2k setup for fluorescence experiment, choosing fluorescence sensors.  
|MitoFit ORO Lab  
|MitoFit ORO Lab  
|-
|-
Line 148: Line 181:
|-
|-
|14:00
|14:00
|Analysis of TMRM/safranin experiment, discussion.  
|Analysis of TMRM/safranin experiment, comparison of TPP+ and fluorescence results.
|MitoFit ORO Lab  
|MitoFit ORO Lab  
|-
|-
Line 156: Line 189:
|-
|-
|16:00
|16:00
|Comparison of TPP+ and fluorescence methods for evaluation of mt-membrane potential.  
|Comparison of TPP+ and fluorescence methods for evaluation of mt-membrane potential. Examples in different protocols.
|MiP''Art''
|MiP''Art''
|-
|-
|17:00
|17:00
|Other potentiometric, amperometric, and fluorometric O2k-MultiSensor applications.  
|Interdependence between O2 flux and mt-membrane potential.  
|MiP''Art''  
|MiP''Art''  
|-
|-
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|}
|}


== Registration ==
:::: [http://wiki.oroboros.at/images/1/14/Registration_Form_Workshop_Innsbruck_2018.pdf Registration form]


== Tutors  and Lecturers==
== Tutors  and Lecturers==
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<br/>
<br/>
<gallery mode=default perrow=6 widths="140px" heights="150px">
<gallery mode=default perrow=6 widths="140px" heights="150px">
File:SumbalovaZ.JPG|'''[[Sumbalova Z|Sumbalova Zuzana]]''', PhD, Medical University of Innsbruck - Principal investigator
File:SumbalovaZ.JPG|'''[[Sumbalova Z|Zuzana Sumbalova ]]''', PhD, Medical University of Innsbruck - Principal investigator
File:Chang SC.JPG|'''[[Chang SC|Chang Shao-Chiang]]''' MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student [[TRACT]]
File:Chang SC.JPG|'''[[Chang SC|Shao-Chiang Chang ]]''' MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student [[TRACT]]
File:PassruggerM.JPG|'''[[Passrugger M|Passrugger Manuela]]''', BSc., Oroboros Instruments - Biomedical Assistant  
File:PassruggerM.JPG|'''[[Passrugger M|Manuela Passrugger ]]''', BSc., Oroboros Instruments - Biomedical Assistant  
Image:Bastos Sant'Anna Silva AC.jpg|'''[[Bastos Sant'Anna Silva AC|Ana Carolina Bastos Sant'Anna Silva]]''', MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student [[TRANSMIT]]
Image:Bastos Sant'Anna Silva AC.jpg|'''[[Bastos Sant'Anna Silva AC|Ana Carolina Bastos Sant'Anna Silva]]''', MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student [[TRANSMIT]]
File:Gnaiger Erich.jpg|'''[[Gnaiger E|Erich Gnaiger]]''', Prof., CEO Oroboros Instruments & Medical University of Innsbruck, D. Swarovski Research Laboratory
|-
|-
</gallery>
</gallery>


'''Lecturers and assistance'''
<br/>
<gallery mode=default perrow=6 widths="140px" heights="150px">
File:DoerrierC.JPG|'''[[Doerrier C|Doerrier Carolina]]''',PhD, Oroboros Instruments - Chief Scientific Officer
File:KomlodiT.JPG|'''[[Komlodi T|Komlodi Timea]]''',Dr., Oroboros Instruments - Scientific Researcher
File:MeszarosA.JPG| '''[[Meszaros A|Meszaros Andras]]''', MD, PhD, Oroboros Instruments - Postdoctoral researcher
|-
</gallery>


== List of participants ==
== List of participants ==
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| [[Fernandez-Vizarra E|Fernandez-Vizarra Erika]]
| [[Fernandez-Vizarra E|Fernandez-Vizarra Erika]]
| '''UK Cambridge Zeviani M''' - University of Cambridge
| '''UK Cambridge Zeviani M''' - University of Cambridge
|-
| [[File:HungerM.JPG|100px|Hunger Miriam]]
| [[Hunger Miriam]]
| [[AT Innsbruck Oroboros]]
|-
|-
|
|
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| [[Michalak S|Michalak Slawomir]]
| [[Michalak S|Michalak Slawomir]]
| [[PL Poznan Michalak S]] - Poznan University of Medical Sciences
| [[PL Poznan Michalak S]] - Poznan University of Medical Sciences
|-
|
| [[Perez Valencia Juan Alberto]]
| '''BR Rio de Janeiro''' - National Cancer Institute
|-
|-
| [[File:SiewieraK.JPG|100px|Siewiera Karolina]]
| [[File:SiewieraK.JPG|100px|Siewiera Karolina]]
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|-
|-
|}
|}
== Registration ==
:::: closed
== Impressions from the IOC126==
:::: Pictures taken by: [[Meszaros Andras]]
<gallery mode=default perrow=4 widths="350px" heights="350px">
File:IOC126-5.jpg|The participants of the IOC126 during a TPP session in the MiPArt
File:IOC126-4.jpg|[[Sumbalova Zuzana|Zuzana Sumbalova ]], one of the tutors of the IOC126
File:IOC126-7.jpg|Session in the Oroboros MitoFit Laboratory
File:IOC126-8.jpg|The participants [[Michalak Slawomir|Slawomir Michalak ]] and [[Fernandez-Vizarra Erika|Erika Fernandez-Vizarra]] during an experiment
File:IOC126-9.jpg|The tutor [[Chang Shao-Chiang|Shao-Chiang Chang ]] with [[Labieniec-Watala Magdalena|Magdalena Labieniec-Watala]] and [[Perez Valencia Juan Alberto|Juan Alberto Perez Valencia]]
File:IOC-15.jpg|The participants in action
File:IOC126-10.jpg| Tutor [[Passrugger Manuela|Manuela Passrugger]] with [[Steffen Anja|Anja Steffen]]
</gallery>




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:::: Oroboros Instruments
:::: Oroboros Instruments
:::: High-Resolution FluoRespirometry
:::: High-resolution respirometry
:::: Schöpfstrasse 18
:::: Schöpfstrasse 18
:::: A-6020 Innsbruck, Austria
:::: A-6020 Innsbruck, Austria
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::::» '''[[IOC recommended reading]]'''
::::» '''[[IOC recommended reading]]'''
::::» '''[[MitoPedia: Respiratory states]]'''
::::» '''[[MitoPedia: Respiratory states]]'''
:::: [[Image:P.jpg|link=OXPHOS-capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET-capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]


== Project support==
== Project support==
Line 269: Line 314:


{{Labeling
{{Labeling
|additional=ORO, IOC, MitoFit, Next, 2018,  
|additional=ORO, IOC, MitoFit, 2018, TRACT, TRANSMIT
}}
}}


Latest revision as of 15:42, 8 June 2020

                

MiPNet23.01 IOC126 Innsbruck AT

Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126.


Oroboros (2018-01-24) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. Innsbruck, Austria; 2018 Jan 24-26.


• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit


General information

The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (O2k-TPP+ ISE-Module) and fluorescence method (TMRM, safranin/O2k-Fluo LED2-Module). Data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.
data analysis

Feedback

4 feedback forms returned
Question Rating (average)
In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered). 4.50
Balance between practical “hands-on” work and “theoretical” explanations: Please rate from 1 (too much “hands-on”) to 5 (too much theory). 2.88
Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content: 3.50
Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language: 4.25
Level of tutoring during the “hands-on” work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently). 3.25
Quality of tutoring: Please rate from 1 (poor) to 5 (excellent). 4.00
Intensity of the O2k-Course: Please rate from 1 (I would have liked to do and hear more) to 5 (I felt overwhelmed). 3.50
Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent). 4.00

Venue

Information on travel and venue:IOC Innsbruck.
» Hotel suggestion: Hotel Weisses Kreuz
Mention "Oroboros" when booking in order to receive a discount.

Programme

Wednesday 24th Topic Room
09:00 Get-together: Introduction of participants and their research interests. MiPArt
09:30 Erich Gnaiger: Protonmotive force and respiratory control. MitoEAGLE preprint 2017-09-21. MiPArt
10:00 DatLab 7, DL-Protocols. MiPArt
10:30 Coffee/Tea
11:00 Hands-on: Assembly and maintenance of TPP+ and reference electrodes. MiPNet15.03 O2k-MultiSensor-ISE. MitoFit ORO Lab
12:15 Lunch break
13:30 Determination of mt-membrane potential with potentiometric O2k-TPP+ ISE-Module - an introduction. MiPArt
14:30 Hands-on: Set up of the instruments with TPP+ and reference electrodes, O2 air calibration and calibration of TPP+ electrode. MitoFit ORO Lab
16:30 Coffee/Tea
17:00 Hands-on continued: TPP+ chemical background experiment. MiPNet14.05 TPP-mtMembranePotential. MitoFit ORO Lab
Thursday 25th Topic Room
09:00 Hands-on: O2 air calibration and calibration of TPP+ electrode. MitoFit ORO Lab
09:30 Introduction in planning and performing a TPP+ experiment. MitoFit ORO Lab
10:30 Coffee/Tea
11:00 Hands-on continued: TPP+ experiment with mouse brain mitochondria. MitoFit ORO Lab
12:30 Lunch break
14:00 From the TPP+ signal to mt-membrane potential - guide through the Excel templates. TPP-Matematics-MembranePotential. MitoFit ORO Lab
14:30 Analysis of TPP+ experiment. Interdependence between O2 flux and mt-membrane potential. MitoFit ORO Lab
16:00 Coffee/Tea
16:30 The four compartment model - the effect of parameters on calculated mt-membrane potential. The analysis of the model. MiPArt
17:30 Erich Gnaiger: Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. Abstract. MiPArt
Friday 26th Topic Room
09:00 Determination of mt-membrane potential with fluorescence method (TMRM, safranin) - O2k FluoRespirometer - an introduction. MiPNet22.11 O2k-FluoRespirometer manual, MiPNet20.13 Safranin mt-membranepotential. MiPArt
10:00 Hands-on: O2 air calibration and O2k setup for fluorescence experiment, choosing fluorescence sensors. MitoFit ORO Lab
10:30 Coffee/Tea
11:00 Hands-on: Calibration of safranin/TMRM, experiment with mouse brain mitochondria. MitoFit ORO Lab
12:30 Lunch break
14:00 Analysis of TMRM/safranin experiment, comparison of TPP+ and fluorescence results. MitoFit ORO Lab
15:30 Coffee/Tea
16:00 Comparison of TPP+ and fluorescence methods for evaluation of mt-membrane potential. Examples in different protocols. MiPArt
17:00 Interdependence between O2 flux and mt-membrane potential. MiPArt
17:30 The Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions. MiPArt
18:00 Farewell activity MiPArt


Tutors and Lecturers

Tutors


List of participants

Participant Institution
Duicu Oana-Maria RO Timisoara Muntean DM - University of Medicine and Pharmacy
de Kok Michéle NL Leiden Lindeman JH - Leiden University Medical Center
Fernandez-Vizarra Erika UK Cambridge Zeviani M - University of Cambridge
Hunger Miriam Hunger Miriam AT Innsbruck Oroboros
Janssen Connie NL Leiden Lindeman JH - Leiden University Medical Center
Labieniec-Watala Magdalena Labieniec-Watala Magdalena PL Lodz Watala C - University of Lodz
Michalak Slawomir Michalak Slawomir PL Poznan Michalak S - Poznan University of Medical Sciences
Perez Valencia Juan Alberto BR Rio de Janeiro - National Cancer Institute
Siewiera Karolina Siewiera Karolina PL Lodz Watala C - University of Lodz
Smirnova Irina SE Stockholm Smirnova I - Stockholm University
Steffen Anja NL Leiden Lindeman JH - Leiden University Medical Center

Registration

closed

Impressions from the IOC126

Pictures taken by: Meszaros Andras


Contact

[email protected]
Oroboros Instruments
High-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Sponsors.png


Labels:






ORO, IOC, MitoFit, 2018, TRACT, TRANSMIT 


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