Michalak Slawomir

News and Events

Working Groups

Short-Term Scientific Missions

Management Committee

Members

COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

Michalak Slawomir

MitoPedia topics: EAGLE

COST: Member

COST WG4: WG4 COST Mentor: Mentor

MC Member - Management Committee MitoEAGLE

Name	Michalak Slawomir, Dr
Institution	Department of Neurochemistry and Neuropathology, Poznan University of Medical Sciences, PL
Address	Przybyszewskiego Str. 49, 60-355
City	Poznan
State/Province
Country	Poland
Email	[email protected]
Weblink
O2k-Network Lab	PL Poznan Michalak S

Labels: Field of research: Basic, Clinical

Publications

	Published	Reference
MitoEAGLE blood cells 1	2020	Åsander Frostner Eleonor, Aburel Oana M, Avram Vlad F, Calabria Elisa, Castelo Rueda Maria Paulina, Chamkha Imen, Čižmárová Beata, Danila Maria-Daniela, Doerrier Carolina, Eckert Gunter P, Ehinger Johannes K, Elmer Eskil, Garcia-Souza Luiz F, Gnaiger Erich, Hoppel Florian, Karabatsiakis Alexander, Keppner Gloria, Kidere Dita, Krako Jakovljević Nina, Labieniec-Watala Magdalena, Lelcu Theia, Micankova Petra, Michalak Slawomir, Molina Anthony JA, Pavlovic Kasja, Pichler Irene, Piel Sarah, Rousar Tomas, Rybacka-Mossakowska Joanna, Schartner Melanie, Siewiera Karolina, Silaidos Carmina, Sjövall Fredrik, Sobotka Ondrej, Sumbalova Zuzana, Swiniuch Daria, Vernerova Andrea, Volani Chiara, Vujacic-Mirski Ksenija, Watala Cezary (2020) Interlaboratory guide to mitochondrial respiratory studies with peripheral blood mononuclear cells and platelets. - Updated: 2020-03-06 - *Confirmed
BEC 2020.1 doi10.26124bec2020-0001.v1	2020	Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
Michalak 2017 Oxid Med Cell Longev	2017	Michalak S, Florczak-Wyspiańska J, Rybacka-Mossakowska J, Ambrosius W, Osztynowicz K, Baszczuk A, Kozubski W, Wysocka E (2017) Mitochondrial respiration in intact peripheral blood mononuclear cells and sirtuin 3 activity in patients with movement disorders. Oxid Med Cell Longev 2017:9703574.

Abstracts

	Published	Reference
Calabria 2019 MiPschool Coimbra	2019	Investigating mitochondrial function in blood cells by high-resolution respirometry: contribution to an interlaboratory study. MitoEAGLE WG4.
Michalak 2019 MiP2019	2019	No abstract.
Michalak 2018 MiP2018	2018	No abstract.
Michalak 2017 Abstract MITOEAGLE Barcelona	2017	Mitochonrial respiration in intact peripheral blood mononuclear cells in patients with extrapyramidal syndromes.
Michalak 2017 MiP2017	2017	The effect of anticoagulants on mitochondrial respiration in intact peripheral blood mononuclear cells.
Rybacka-Mossakowska 2017 MiP2017	2017	Mitochondrial respiration in intact peripheral blood mononuclear cells in multiple sclerosis patients.

MitoEAGLE Short-Term Scientific Mission

- - - STSM Grant Period 2

Work plan summary

1. Aim and motivation. Blood cells, like peripheral mononuclear cells (PBMCs) and platelets, can be obtained with minimal invasive procedures and used for analyzes of mitochondrial respiration for diagnostic and research purposes. Up to now I have analyzed by means of ELISA technique the expression of mitochondrial proteins in PBMCs from movement disorders and cancer patients. With the use of high-resolution respirometry I have analyzed mitochondrial respiration in PBMCs in movement disorders and cancer patients. The results were already published. Moreover, I have performed methodological study on the effects of anticoagulants on mitochondrial respiration in PBMCs. The results of the studies in movement disorders patients and on anticoagulant effects were my contributions to the Working Group 4 in COST Action MITOEAGLE. Currently, I do have the opportunity to perform analyzes of Ca+2 ions and mitochondrial membrane potential basing on tetraphenylphosphonium (TPP+) or TPMP+ evaluations using potentiometric ion-selective electrodes (ISE) and of reactive oxigen species using the system for high-resolution respirometry (HRR). However, ISE and HRR techniques require specific skills I would like to acquire during STSM I apply to and it is the aim of this STMS.

2. Proposed contributions to the scientific objectives of the Action I will contribute the results of the studies on calcium flow, mitochondrial membrane potential and production of reactive oxigen species in PBMCs in physiological and pathological conditions to the repository of Working Group 4 in COST Action MITOEAGLE. Moreover, I will continue the studies on methodology and protocols for mitochondrial analyzes in PBMCs using new techniques I will learn during STSM.

3. Techniques The techniques I will learn during STSM include potentiometric analyses using ion-selective electrodes (ISE) and high-resolution respirometry by means of MultiSensor applications. My training during STSM will fully contribute to the scientific objectives of MitoEAGLE according to the Memorandum of Understanding.

MitoEAGLE feedback

I would like to participate in WG4 - MitoEAGLE data repository for blood cells and clutured cells. We are already working with cryopreserved PBMC from lung cancer, breast cancer and colon cancer patients. Thus, I would like to participate in WG4 tasks. - Slawomir Michalak (2016).

Participated at

Visiting scientist in the Oroboros O2k-Laboratory

Slawomir Michalak : Visiting scientist at the Oroboros O2k-Laboratory

January 23 to February 03 2018