MiPNet23.01 IOC126 Innsbruck AT
 Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126. |
Oroboros (2018-01-24) Mitochondr Physiol Network
Abstract:
Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. Innsbruck, Austria; 2018 Jan 24-26.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (O2k-TPP+ ISE-Module) and fluorescence method (TMRM, safranin/O2k-Fluo LED2-Module). Data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.
Feedback
- 4 feedback forms returned
| Question | Rating (average) |
|---|---|
| In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered). | 4.50 |
| Balance between practical “hands-on” work and “theoretical” explanations: Please rate from 1 (too much “hands-on”) to 5 (too much theory). | 2.88 |
| Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content: | 3.50 |
| Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language: | 4.25 |
| Level of tutoring during the “hands-on” work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently). | 3.25 |
| Quality of tutoring: Please rate from 1 (poor) to 5 (excellent). | 4.00 |
| Intensity of the O2k-Course: Please rate from 1 (I would have liked to do and hear more) to 5 (I felt overwhelmed). | 3.50 |
| Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent). | 4.00 |
Venue
- Information on travel and venue:IOC Innsbruck.
- » Hotel suggestion: Hotel Weisses Kreuz
- Mention "Oroboros" when booking in order to receive a discount.
Programme
Wednesday 24th Topic Room 09:00 Get-together: Introduction of participants and their research interests. MiPArt 09:30 Erich Gnaiger: Protonmotive force and respiratory control. MitoEAGLE preprint 2017-09-21. MiPArt 10:00 DatLab 7, DL-Protocols. MiPArt 10:30 Coffee/Tea 11:00 Hands-on: Assembly and maintenance of TPP+ and reference electrodes. MiPNet15.03 O2k-MultiSensor-ISE. MitoFit ORO Lab 12:15 Lunch break 13:30 Determination of mt-membrane potential with potentiometric O2k-TPP+ ISE-Module - an introduction. MiPArt 14:30 Hands-on: Set up of the instruments with TPP+ and reference electrodes, O2 air calibration and calibration of TPP+ electrode. MitoFit ORO Lab 16:30 Coffee/Tea 17:00 Hands-on continued: TPP+ chemical background experiment. MiPNet14.05 TPP-mtMembranePotential. MitoFit ORO Lab
Thursday 25th Topic Room 09:00 Hands-on: O2 air calibration and calibration of TPP+ electrode. MitoFit ORO Lab 09:30 Introduction in planning and performing a TPP+ experiment. MitoFit ORO Lab 10:30 Coffee/Tea 11:00 Hands-on continued: TPP+ experiment with mouse brain mitochondria. MitoFit ORO Lab 12:30 Lunch break 14:00 From the TPP+ signal to mt-membrane potential - guide through the Excel templates. TPP-Matematics-MembranePotential. MitoFit ORO Lab 14:30 Analysis of TPP+ experiment. Interdependence between O2 flux and mt-membrane potential. MitoFit ORO Lab 16:00 Coffee/Tea 16:30 The four compartment model - the effect of parameters on calculated mt-membrane potential. The analysis of the model. MiPArt 17:30 Erich Gnaiger: Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. Abstract. MiPArt
Friday 26th Topic Room 09:00 Determination of mt-membrane potential with fluorescence method (TMRM, safranin) - O2k FluoRespirometer - an introduction. MiPNet22.11 O2k-FluoRespirometer manual, MiPNet20.13 Safranin mt-membranepotential. MiPArt 10:00 Hands-on: O2 air calibration and O2k setup for fluorescence experiment, choosing fluorescence sensors. MitoFit ORO Lab 10:30 Coffee/Tea 11:00 Hands-on: Calibration of safranin/TMRM, experiment with mouse brain mitochondria. MitoFit ORO Lab 12:30 Lunch break 14:00 Analysis of TMRM/safranin experiment, comparison of TPP+ and fluorescence results. MitoFit ORO Lab 15:30 Coffee/Tea 16:00 Comparison of TPP+ and fluorescence methods for evaluation of mt-membrane potential. Examples in different protocols. MiPArt 17:00 Interdependence between O2 flux and mt-membrane potential. MiPArt 17:30 The Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions. MiPArt 18:00 Farewell activity MiPArt
Tutors and Lecturers
Tutors
Zuzana Sumbalova , PhD, Medical University of Innsbruck - Principal investigator
Shao-Chiang Chang MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRACT
Manuela Passrugger , BSc., Oroboros Instruments - Biomedical Assistant
Ana Carolina Bastos Sant'Anna Silva, MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRANSMIT
Erich Gnaiger, Prof., CEO Oroboros Instruments & Medical University of Innsbruck, D. Swarovski Research Laboratory
List of participants
| Participant | Institution | |
|---|---|---|
| Duicu Oana-Maria | RO Timisoara Muntean DM - University of Medicine and Pharmacy | |
| de Kok Michéle | NL Leiden Lindeman JH - Leiden University Medical Center | |
| Fernandez-Vizarra Erika | UK Cambridge Zeviani M - University of Cambridge | |
|
Hunger Miriam | AT Innsbruck Oroboros |
| Janssen Connie | NL Leiden Lindeman JH - Leiden University Medical Center | |
|
Labieniec-Watala Magdalena | PL Lodz Watala C - University of Lodz |
|
Michalak Slawomir | PL Poznan Michalak S - Poznan University of Medical Sciences |
| Perez Valencia Juan Alberto | BR Rio de Janeiro - National Cancer Institute | |
|
Siewiera Karolina | PL Lodz Watala C - University of Lodz |
| Smirnova Irina | SE Stockholm Smirnova I - Stockholm University | |
| Steffen Anja | NL Leiden Lindeman JH - Leiden University Medical Center |
Registration
- closed
Impressions from the IOC126
- Pictures taken by: Meszaros Andras
The participants of the IOC126 during a TPP session in the MiPArt
Zuzana Sumbalova , one of the tutors of the IOC126
Session in the Oroboros MitoFit Laboratory
The participants Slawomir Michalak and Erika Fernandez-Vizarra during an experiment
The tutor Shao-Chiang Chang with Magdalena Labieniec-Watala and Juan Alberto Perez Valencia
The participants in action
Tutor Manuela Passrugger with Anja Steffen
Contact
- Oroboros Instruments
- High-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Labels:
ORO, IOC, MitoFit, 2018, TRACT, TRANSMIT
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
