MiPNet09.12 O2k-Titrations: Difference between revisions
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|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. | |title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. | ||
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/e/ef/MiPNet09.12_O2k-Titrations.pdf |Bioblast pdf]] | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/e/ef/MiPNet09.12_O2k-Titrations.pdf |Bioblast pdf]] | ||
|authors= | |authors=Oroboros | ||
|year=2016-09-30 | |year=2016-09-30 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=''' | |abstract='''Oroboros (2016) Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(15): 1-2.'''ย ยป[http://www.bioblast.at/index.php/File:MiPNet09.12_O2k-Titrations.pdf Versions] | ||
A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations. | A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations. | ||
:ยป [[O2k-Protocols]] | :ยป [[O2k-Protocols]] | ||
:ยป Product: [[ | :ยป Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue |O2k-Catalogue]] | ||
|mipnetlab= | |mipnetlab=AT_Innsbruck_Oroboros | ||
}} | }} | ||
{{Labeling | {{Labeling |
Revision as of 15:55, 2 August 2017
Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. |
Oroboros (2016-09-30) Mitochondr Physiol Network
Abstract: Oroboros (2016) Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(15): 1-2. ยปVersions
A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.
- ยป O2k-Protocols
- ยป Product: Oroboros O2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_Oroboros
Labels: MiParea: Instruments;methods
Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid
HRR: O2k-Protocol
O2k-chemicals and media
Note: Titration of chemicals in respirometry
Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.
Further information
- ยป MitoPedia: SUIT - Substrate-uncoupler-inhibitor-titration (SUIT) protocols for respirometry
- ยป MiPNet03.02 - Chemicals and media for respirometry
- ยป MiPNet19.14 - SOP for manual O2k-titrations with Hamilton microsyringes.