Vera Vives 2022 MitoFit

Vera-Vives AM, Perin G, Morosinotto T (2022) The robustness of NextGen-O2k for building PI curves in microalgae. MitoFit Preprints 2022.19. https://doi.org/10.26124/mitofit:2022-0019

»

The robustness of NextGen-O2k for building PI curves in microalgae

Vera Vives Antoni Mateu, Perin Giorgio, Morosinotto Tomas (2022-05-12) MitoFit Prep

Abstract: The rate of oxygen evolution provides valuable information on the metabolic status and the photosynthetic performance of a cell, and it can be quantified by means of a photosynthesis-irradiance (PI) curve. Up to now, the construction of PI curves of unicellular organisms based on oxygen evolution has been difficult and time consuming due to the lack of sensitive instruments. Here we describe the set up of a reproducible method for constructing PI curves based on oxygen evolution using low amounts of sample in the microalga Nannochloropsis gaditana, easily translable to other algal species. • Keywords: photosynthesis, microalgae, oxygen evolution • Bioblast editor: Tindle-Solomon L

Labels:

Organism: Plants 

Preparation: Chloroplasts 

HRR: Oxygraph-2k, NextGen-O2k 

Bioblast 2022 

ORCID: Vera Vives Antoni Mateu, Perin Giorgio, Morosinotto Tomas