User talk:Tindle Lisa/My sandbox Support page v2: Difference between revisions

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<big><big>'''"A reprogramming-based strategy for drug repositioning in patients with mitochondrial DNA-associated Leigh syndrome (MILS)"'''</big></big>
:::::::::::: <big>''Currently Asked Questions - »'''[[O2k-Open Support alert]]'''« </big>  


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== O2k-Open Support resources ==
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'''Startup information'''
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:::: Each O2k-Package includes a remote installation and startup support session with an Oroboros expert, which can be scheduled during any time within the O2k-Warranty period (max. 2 years)
:::::» '''[https://www.oroboros.at/index.php/support-session-request-form/ Register for your session]'''
:::::» [[Installation and startup support session self-study material]]
:::: Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.
:::::» [[O2k on-site training]]


__TOC__
:::: Additional reading
:::::» [[MiPNet20.04 O2k-checklist| Getting started with an O2k-experiment]]
:::::» O2k-Workshop self-study material: [https://wiki.oroboros.at/index.php/Virtual_O2k-Workshop_self-study_material#O2k-Basic O2k-Basic]
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'''Quality control'''
 
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[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].     
 
:::: The '''O<sub>2</sub> sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].
::::# Clean O2k-chambers and contamination-free incubation medium are required.
::::# Analysis of DatLab files recorded in instrumental QC tests is essential.       
::::# Biological experiments are not suitable for trouble shooting.
 
:::: '''1. O<sub>2</sub> sensor test'''
:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
::::» O<sub>2</sub> sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]     
 
:::: '''2. O2k-chamber test'''
:::: If all quality control criteria of the O<sub>2</sub> sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background test''']]     
 
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'''Advanced topics'''
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::» O2k-Workshop self-study material: [https://wiki.oroboros.at/index.php/Virtual_O2k-Workshop_self-study_material#O2k-Advanced O2k-Applications: Simultaneous determination of O2 and H2O2 fluxes]


:: You may also find the answers to more advanced topics using the navigation menu above or simply by using the Bioblast search function.:
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== "Project Name": "Funding Scheme" ==
| [[Image:O2k-GlossaryFaq icon.jpg|50px|link=https://wiki.oroboros.at/index.php/MitoPedia:_O2k-Open_Support |MitoPedia: O2k-Open Support]]
::::*  EJP RD JTC 2020: "PRE-CLINICAL RESEARCH TO DEVELOP EFFECTIVE THERAPIES FOR RARE DISEASES” - Project EJPRD20-010
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::::* '''Duration''': 36 months
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::::* '''Start''': 
::::* '''Web''':


== About CureMILS ==
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:::: Mitochondrial DNA (mtDNA)-associated Leigh syndrome (MILS) is a severe early-onset brain disease affecting 1/100,000 newborns. MILS is typically caused by mtDNA mutations in the ATP-generating subunit MT-ATP6. There are no treatments available for MILS. In fact, drug discovery is particularly challenging for MILS. The limited access to patient neural tissue and the difficulty to manipulate mtDNA hinder the development of transgenic animal models and cellular models, which are needed for treatment discovery and development. Led by Prof. Dr. Alessandro Prigione of the Heinrich Heine University, the CureMILS project aims to employ a novel approach to enable drug discovery of MILS.
::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, with lists of key words and links to relevant pages.
::::::## [[MitoPedia: O2k hardware]]
::::::## [[MitoPedia: DatLab]]
::::::## [[MitoPedia:_SUIT ]]
::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
::::::# [[O2k-Manual]]: topic specific manuals with step by step help
::::::# [[O2k-Videosupport]]: provides valuable assistance, complementary to the O2k-Manual
::::::# [[MitoPedia: O2k-Open Support|O2k-Glossary]]: full list of key words and links to relevant pages.


===Aims===
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:::: Our consortium will employ neural cells generated from MILS patients via cellular reprogramming to carry out a large-scale screening using marketed drugs, thereby allowing the identification of therapeutic strategies.
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== Ask an O2k-Expert and the O2k-Open Support agreement ==


:::: Our proof-of-concept study demonstrated that this approach is feasible and relevant. We propose to extend this approach using a large high-quality library of repurposable compounds (more than 5,500). We will validate hit compounds by combining mitochondrial profiling with multi-omics analysis using various reprogramming-derived neural models (neural progenitors, neurons, brain organoids, and blood-brain barrier cells) from different MILS patients.
:::: We give you the tools to be self-sufficient, but sometimes you need expert advice or help solving a technical problem. The Oroboros experts will be happy to help in using our O2k-Open Support system. » [https://www.oroboros.at/index.php/o2k-technical-support '''Contact Support''']     
===Objectives===
::::» [[O2k-Open Support agreement]]
:::: Our consortium will identify drugs suited for repositioning as interventions in MILS, laying the foundation for a multi-national clinical trial and a concrete path towards a cure for MILS.
::::» [[O2k repair]]


:::: Moreover, we will establish a paradigmatic working pipeline for reprogramming-driven drug discovery and repositioning for rare neurological disorders.
== Coordinator==
::::*  [[Prigione Alessandro| Alessandro Prigione, MD, PhD]] - Department of General Pediatrics, Neonatology, and Pediatric Cardiology at Heinrich Heine University, DE - https://www.neurosciences-duesseldorf.de/principal-investigators-and-junior-researchers/alessandro-prigione OR https://www.hhu.de/en/


[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
== The O2k-Network: An additional resource ==


:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.      
== Network ==
===Consortium members ===
::::* [https://www.ime.fraunhofer.de/en/Research_Divisions/business_fields_TM/screeningport.html Fraunhofer IME, Department ScreeningPort], DE
::::* [https://www.uibk.ac.at/molbiol/index.html.en Institute for Molecular Biology, University of Innsbruck], AT
::::* [https://www.radboudumc.nl/en/research/departments/biochemistry Radboud University Medical Center, Biochemistry], NL
::::* [https://www.helsinki.fi/en/researchgroups/stem-cells-and-metabolism-research-program University of Helsinki, Research Program of Stem Cells and Metabolism], FI
::::* [http://www.ighz.edu.pl/en/ Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Department of Molecular Biology], PL
::::* [https://www.ddsp.univr.it/?ent=home&page=dipartimento&lang=en Verona University, Department of Diagnostics and Public Health], IT
::::* [https://wwwen.uni.lu/lcsb Luxembourg Centre for Systems Biomedicine (LCSB) University of Luxembourg], LU


::::* [https://www.mitopatients.org/ International Mito Patients (IMP)]
::::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]]      
=== Collaborators ===
::::* [https://neuropaediatrie.charite.de/ Charité University, Clinic for Pediatrics and Neurology], DE


::::* [https://www.uniklinik-duesseldorf.de/en/department-of-general-paediatrics Department of General Pediatrics, Neonatology and Pediatric Cardiology, University Children's Hospital, Heinrich Heine University], DE
::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]      


::::* [https://www.oroboros.at/ Oroboros Instruments GmbH], AT
:::: '''»''' [[Oroboros Laboratories|Oroboros O2k-Laboratory]] - open for innovation and cooperation.
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry     


::::* [https://www.istituto-besta.it/ Foundation IRCCS Neurological Institute C. Besta, Unit of Molecular Neurogenetics], IT
[[Category:O2k-Open Support]]


::::* [http://eng.biometra.unimi.it/ecm/home University of Milan, Department of Medical Biotechnology and Translational Medicine], IT


::::* [https://www.molgen.mpg.de/2168/en Max Planck Institute for Molecular Genetics], DE
::::* [http://mitonet.org/en/ Deutsches MITONET e.V., Friedrich-Baur-Institut], DE


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== Oroboros project involvement ==
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:::: Oroboros is a collaborator on the project providing infrastructure and expertise required to conduct respirometric measurements for the project.


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== CureMILS events ==
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== CureMILS publications ==


== CureMILS  dissemination ==


==Links==
===Social Media===
===Research Networks===


== References ==
== Additional information ==
== Support ==
The EJP RD initiative has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement N°825575. EJP RD is coordinated by INSERM, France.
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<div style="margin:0.5em; background:#cedff2; font-family:inherit; font-size:120%; font-weight:bold; border:1px solid #a3b0bf; color:#000; padding:0.2em 0.1em;">Funding acknowledgments</div>
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* [https://www.ejprarediseases.org/index.php/funded-projects-2020/ Funded projects]
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Latest revision as of 13:59, 29 July 2021


                  

O2k-Open Support

User talk:Tindle Lisa/My sandbox Support page v2

Currently Asked Questions - »O2k-Open Support alert« 


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