The protonmotive force and respiratory control: Difference between revisions
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{{MITOEAGLE}} | {{MITOEAGLE}} | ||
[[File:OXPHOS-coupled energy cycles.jpg|right|300px|thumb|OXPHOS-coupled energy cycles. From [[Gnaiger_2014_MitoPathways#Chapter_1._Real-time_OXPHOS_analysis |Gnaiger 2014 MitoPathways]].]] | |||
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::::Β» [[MITOEAGLE protocols, terminology, documentation |WG1 Project application]] | ::::Β» [[MITOEAGLE protocols, terminology, documentation |WG1 Project application]] | ||
[[File: | [[File:EPL-free and excess.jpg|right|300px|thumb|Capacities of the electron transfer system, oxidative phosphorylation and resting LEAK respiration (ETS, OXPHOS, LEAK) and four-compartmental OXPHOS model. (i) Capacity of the ETS module, ''E'', in the noncoupled state, generating the protonmotive force, Ξ''p''<sub>mt</sub>. OXPHOS capacity, ''P'', is partitioned into (ii) the dissipative LEAK component, ''L'' (disspation of Ξ''p''<sub>mt</sub>), and (iii) the free OXPHOS capacity, ''βP=P-L'' (energy conversion driven by Ξ''p''<sub>mt</sub>). If ''βP'' is limited by the capacity of the phosphorylation system, then (iv) the apparent ETS excess capacity, ''ExP=E-P'', is available to drive coupled processes other than phosphorylation without competing with ATP production. Free divided by total ETS capacity, ''βE/E'', is the ETS coupling efficiency. Free divided by total OXPHOS capacity, ''βP/P'', is the OXPHOS coupling efficiency. From [[Gnaiger 2014 MitoPathways]].]] | ||
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== Mitochondrial respiratory coupling control - a conceptual perspective on terminology == | == Mitochondrial respiratory coupling control - a conceptual perspective on terminology == | ||
::: '''Scope of MITOEAGLE publication: Respiratory states''' Β | ::: '''Scope of MITOEAGLE publication: Respiratory states''' Β | ||
::::* Target a broad audience β also the new generation | ::::* Target a broad audience β also the new generation | ||
::::* List of terms including historical terms; abbreviations (mtDNA, mt to abbreviate mitochondr*) | ::::* List of terms including historical terms; abbreviations (mtDNA, mt to abbreviate mitochondr*); OXPHOS capacity versus State 3 (discuss saturating ADP/Pi .. concentrations) | ||
::::* Scientific terminology should be general and platform independent - demands of the working groups | ::::* Scientific terminology should be general and platform independent - demands of the working groups | ||
| Line 34: | Line 34: | ||
== References == | == References == | ||
::::* [[Gnaiger_2014_MitoPathways#Chapter_2._Respiratory_states:_coupling_control |Gnaiger 2014 MitoPathways]] | |||
::::* Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of oxidative phosphorylation by temperature in the heart. bioRxiv doi10.1101/103457. - [[Lemieux 2017 bioRxiv |Β»Bioblast linkΒ«]] | ::::* Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of oxidative phosphorylation by temperature in the heart. bioRxiv doi10.1101/103457. - [[Lemieux 2017 bioRxiv |Β»Bioblast linkΒ«]] | ||
::::* [[Miller 1991 Scientific American Library]] | ::::* [[Miller 1991 Scientific American Library]] | ||
::::* http://www.nature.com/cdd/journal/v16/n1/full/cdd2008150a.html | ::::* http://www.nature.com/cdd/journal/v16/n1/full/cdd2008150a.html | ||
::::* http://www.nature.com/cdd/journal/v20/n7/full/cdd201327a.html | ::::* http://www.nature.com/cdd/journal/v20/n7/full/cdd201327a.html | ||
== Next steps == | |||
::: Mitochondrial respiratory pathway control - substrates and inhibitors | |||
:::: Switch to pathway-related nomenclature instead of enzyme-linked terminology (N/NS/S versus CI/CI+II/CII) | |||
Revision as of 08:51, 10 April 2017
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Working Groups | Short-Term Scientific Missions | Management Committee | Members |  |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
The protonmotive force and respiratory control
OXPHOS-coupled energy cycles. From Gnaiger 2014 MitoPathways.
- Β» WG1 Action - WG1 MITOEAGLE protocols, terminology, documentation: Standard operating procedures and user requirement document: Protocols, terminology, documentation
- Β» WG1 Project application
Capacities of the electron transfer system, oxidative phosphorylation and resting LEAK respiration (ETS, OXPHOS, LEAK) and four-compartmental OXPHOS model. (i) Capacity of the ETS module, E, in the noncoupled state, generating the protonmotive force, Ξpmt. OXPHOS capacity, P, is partitioned into (ii) the dissipative LEAK component, L (disspation of Ξpmt), and (iii) the free OXPHOS capacity, βP=P-L (energy conversion driven by Ξpmt). If βP is limited by the capacity of the phosphorylation system, then (iv) the apparent ETS excess capacity, ExP=E-P, is available to drive coupled processes other than phosphorylation without competing with ATP production. Free divided by total ETS capacity, βE/E, is the ETS coupling efficiency. Free divided by total OXPHOS capacity, βP/P, is the OXPHOS coupling efficiency. From Gnaiger 2014 MitoPathways.
Mitochondrial respiratory coupling control - a conceptual perspective on terminology
- Scope of MITOEAGLE publication: Respiratory states
- Target a broad audience β also the new generation
- List of terms including historical terms; abbreviations (mtDNA, mt to abbreviate mitochondr*); OXPHOS capacity versus State 3 (discuss saturating ADP/Pi .. concentrations)
- Scientific terminology should be general and platform independent - demands of the working groups
- Scope of MITOEAGLE publication: Respiratory states
- Structure
- From bioenergetics to mitochondrial physiology - historical view
- The mitochondrial respiratory system
- Rates and states - Units (important for a database); analogous to electic terms: Flow [C.s-1]; Flux [C.s-1.m-2]; Rate (?)
- Intact cells, mt preparation and normalization
- Coupling states: mt-preparations and intact cells
- Pathway states: mt-preparations and intact cells
- References
- Structure
- Journal
- Int J Biochem Cell Biol (W Koopman will be the new editor); Open Access is a requirement
- Journal
Action
References
- Gnaiger 2014 MitoPathways
- Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of oxidative phosphorylation by temperature in the heart. bioRxiv doi10.1101/103457. - Β»Bioblast linkΒ«
- Miller 1991 Scientific American Library
- http://www.nature.com/cdd/journal/v16/n1/full/cdd2008150a.html
- http://www.nature.com/cdd/journal/v20/n7/full/cdd201327a.html
Next steps
- Mitochondrial respiratory pathway control - substrates and inhibitors
- Switch to pathway-related nomenclature instead of enzyme-linked terminology (N/NS/S versus CI/CI+II/CII)
- Mitochondrial respiratory pathway control - substrates and inhibitors
