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Oxygraph-2k

» OROBOROS Oxygraph-2k: the modular system for high-resolution respirometry and mitochondrial physiology

OROBOROS O2k-Catalogue - a guided tour to the O2k

From the O2k to the Power-O2k

Multiple O2k-units are offered as a ‘Power-O2k System’ for HRR combined with high-throughput - at high output.

O2k-technical support and open innovation

O2k-Manual

Instructions to application and service of the O2k-Core, TIP2k and O2k-MultiSensor Modules.

O2k-Protocols

O2k-Protocols are complementary to the O2k-Manual and O2k-Publications. Users are encouraged to get involved, contribute and share their expertise.

»4710 O2k-Publications are part of the category Publications, providing a portal for sharing, disseminating and commenting relevant literature in mitochondrial physiology. Bioblast allows the “evolution” of a publication with space for open discussions and extensions of an otherwise static ‘paper’. This applies specifically to Methods and Materials, Discussion, and References sections, which may be limited by space in original publications.

OROBOROS-Events: O2k-Workshops and Events

Join us in the fast lane to high-resolution respirometry. While the OROBOROS O2k provides the instrumental basis for HRR, successful operation at high accuracy up to the limit of detection depends on a professional application by the technician, scientist or student. Participation in our hands-on introductory OROBOROS O2k-Workshops is recommended to save time and gain from the experience obtained over many years of application in various settings.

O2k-Network

O2k-Network Labs from all over the world join to form the unique OROBOROS O2k-Network for high-resolution respirometry.

The world as a laboratory - science and adventure

Science and adventure brings the OROBOROS-Team and the O2k to many and sometimes extreme parts of the world in international collaborations - The world as a laboratory.

Mitochondr Physiol Network

Mitochondrial Physiology Network - the OROBOROS on-line journal with Open Access in the spirit of Gentle Science.

O2k-Feedback

OROBOROS Company - OROBOROS-Team

OROBOROS Contact

Company of Scientists

http://www.company-of-scientists.com

Company of Scientists

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-[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|left|150px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |OROBOROS]]
+=== OROBOROS navigation ===
-[[OROBOROS INSTRUMENTS]] distributes the world-wide leading O2k-technology of high-resolution respirometry - '''O2k and HRR'''. The [[OROBOROS Company]] is fully scientifically oriented, with emphasis on continuous innovation. The extension of the [[Oxygraph-2k]] (O2k) to the [[O2k-Fluorometer]] has set a new standard for O2k-MultiSensor approaches (see [[OROBOROS O2k-Catalogue]]). The O2k is established internationally, with [[O2k-Publications: Topics |»{{#ask:[[Category:Publications]][[Instrument and  method::Oxygraph-2k]] |format=count}} O2k-Publications]] in the scientific literature. OROBOROS has organized more than 100 international [[OROBOROS_Events#Next_O2k-Workshops |O2k-Workshops]]. The [[O2k-Network]] includes more than 350 Reference Laboratories in 45 countries.
-=== The [[O2k]] for high-resolution respirometry ===
-[[Image:O2k-Fluorometer.JPG|left|150px|link=http://wiki.oroboros.at/index.php/Oxygraph-2k |O2k]]
-:: O2k is the acronym for the OROBOROS '''[[Oxygraph-2k]]''' and '''[[O2k-Fluorometer]]''' - the modular system for high-resolution respirometry and mitochondrial physiology. The O2k provides a unique high-resolution approach for the monitoring of cellular and mitochondrial respiratory function: [[high-resolution respirometry |high-resolution respirometry (HRR)]]. Considering mitochondria as the powerhouses of the cell, HRR introduced the concept of [[cell ergometry]]. Are your cells [[MitoFit]]?
-HRR is applied [[O2k-Network |world-wide]] in areas ranging from fundamental bioenergetics to the analysis of mitochondrial and metabolic diseases, advancing the rapidly growing field of preventive mitochondrial medicine. Mitochondrial fitness needs to be monitored in a variety of age-related health risks, including cardiovascular and degenerative diseases, such as type 2 diabetes, neurodegenerative diseases (Alzheimer’s, Parkinson’s, Huntington’s), and several types of cancer.
-==== [[OROBOROS MitoFit Laboratory]] ====
-[[File:OROBOROS MitoFit-Lab.jpg|left|240px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |OROBOROS]]
-HRR by the OROBOROS O2k is based on a radically different systems approach to polarographic measurement of oxygen dynamics. HRR rests on several tightly connected instrumental features and concepts integrated by the DatLab software, providing real-time display of oxygen concentration and respiration ([[oxygen flux]]). The mechanics, critical selection of materials, and state-of-the-art electronics provide the basis of signal stability of an optimized [[polarographic oxygen sensor]], in an electronically controlled thermal environment with high temperature stability. Calibrations and instrumental controls follow standard operating procedures, with highly automatic real-time analysis by the DatLab software. Scientific support is complementary to the approach of HRR.
-==== [[Cell ergometry]] ====
-[[File:EPL-free and excess.jpg|left|240px|link=Gnaiger 2014 MitoPathways |Gnaiger 2014 MitoPathways]]
-::* '''»''' A concept complete with elaborated protocols, to get started and gain competence in the expanding field of bioenergetics: » [[Gnaiger 2014 MitoPathways |Blue Book 2014]] - » [[O2k-Protocols]].
-::* '''»''' Explore cell respiration and key mitochondrial functions''': [[High-resolution respirometry |» HRR]] and the [http://www.oroboros.at/?Oxygraph » O2k].
-::* '''»''' Introductory courses and advanced training: » [[OROBOROS_Events]].
-::'''»'''  [http://www.oroboros.at/?Purchase WorldWide distribution].
-=== The O2k concept ===
 [[Image:Oxygraph-2k-Concept.jpg|250px|right|thumb|link=http://wiki.oroboros.at/index.php/Oxygraph-2k|Oxygraph-2k]]
 <big>'''» [[Oxygraph-2k | OROBOROS Oxygraph-2k]]</big>: the modular system for [[high-resolution respirometry]]''' and mitochondrial physiology