Talk:MiPNet20.14 AmplexRed H2O2-production: Difference between revisions
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::: [[MiPNet20.14 AmplexRed H2O2-production]] has been accessed more than Β | ::: [[MiPNet20.14 AmplexRed H2O2-production]] has been accessed more than Β | ||
::::*Β 5,000 times (2016-06-29) | ::::*Β 5,000 times (2016-06-29) | ||
[[High-resolution respirometry]] is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: [[O2k-Publications:_O2k-Fluorometry_-_Fluorophores|H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels]]. | |||
Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber: | |||
# Quality control of preparations by respirometric performance. | |||
# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores. | |||
# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber. | |||
# Additional information is acquired for a limited amount of sample. | |||
# Oxygen dependence of parameters can be studied. | |||
In the publication by [[Krumschnabel_2015_Methods_Mol_Biol|Krumschnabel et al. 2015]] we introduce the O2k-Fluo LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H<sub>2</sub>O<sub>2</sub> production of mouse brain mitochondria. The paper by [[Makrecka-Kuka_2015_Biomolecules|Makrecka-Kuka et al. 2015]] focuses on the application of the Amplex red assay for determining H<sub>2</sub>O<sub>2</sub> production in three different models of mitochondrial preparations. | |||
'''O2k-Publication''': | |||
[[Image:O2k-Publications.jpg|50px|link=O2k-Publications:_Topics|O2k-Publications in the MiPMap]] | |||
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. [[Krumschnabel 2015 Methods Mol Biol |Β»Bioblast linkΒ«]] | |||
[[Image:O2k-Publications.jpg|50px|left|link=O2k-Publications:_Topics|O2k-Publications in the MiPMap]] | |||
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. [[Makrecka-Kuka 2015 Biomolecules| Β»Bioblast linkΒ«]] |
Revision as of 16:45, 15 November 2019
Popular Bioblast page
- MiPNet20.14 AmplexRed H2O2-production has been accessed more than
- 5,000 times (2016-06-29)
- MiPNet20.14 AmplexRed H2O2-production has been accessed more than
High-resolution respirometry is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H2O2 production, mitochondrial membrane potential, intracellular pH, Ca2+, Mg2+, and NADH levels. Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
- Quality control of preparations by respirometric performance.
- Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP+ or fluorophores.
- Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
- Additional information is acquired for a limited amount of sample.
- Oxygen dependence of parameters can be studied.
In the publication by Krumschnabel et al. 2015 we introduce the O2k-Fluo LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H2O2 production of mouse brain mitochondria. The paper by Makrecka-Kuka et al. 2015 focuses on the application of the Amplex red assay for determining H2O2 production in three different models of mitochondrial preparations.
O2k-Publication:
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. Β»Bioblast linkΒ«
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. Β»Bioblast linkΒ«