Description
A system is in a steady state if the levels of a specific variable (e.g. O2 flux, H2O2 flux, mitochondrial membrane potential) in the system do not change over time. High-resolution respirometry allows for the measurement of different parameters (e.g. O2 flux, H2O2 flux, mitochondrial membrane potential) at a pseudo-steady state, when changes of concentrations in the closed system do not exert any control on fluxes. Combination with the Titration-Injection microPump (TIP2k) allows operation with programmable titration regimes and at quasi steady-states, yielding an expanded flexibility in experimental design by combining the technical advantages of closed and open systems approaches.
Reference: Gnaiger 2000 Proc Natl Acad Sci U S A, Gnaiger 2001 Respir Physiol, Harrison 2015 J Appl Physiol
Communicated by Doerrier C (2020-04-20) last update 2020-05-28
DatLab oxygen flux: performance and data analysis
References
Bioblast link | Reference | Year |
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Chance 1955 J Biol Chem-III | Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation: III. The steady state. J Biol Chem 217:409-27. | 1955 |
Donnelly 2023 MitoFit | Donnelly C, Komlรณdi T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-0002 โ 2024-11-17 published in Redox Biol. | 2023 |
Gnaiger 1993 Pure Appl Chem | Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65:1983-2002. http://dx.doi.org/10.1351/pac199365091983 | 1993 |
Gnaiger 2001 Respir Physiol | Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. https://doi.org/10.1016/S0034-5687(01)00307-3 | 2001 |
BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al โ MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
Gnaiger 2000 Proc Natl Acad Sci U S A | Gnaiger E, Mรฉndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5. https://doi.org/10.1073/pnas.97.20.11080 | 2000 |
Harrison 2015 J Appl Physiol | Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol 119:1210-8. https://doi.org/10.1152/japplphysiol.00146.2015 | 2015 |
MitoPedia concepts: "MitoFit Quality Control System" is not in the list (MiP concept, Respiratory state, Respiratory control ratio, SUIT concept, SUIT protocol, SUIT A, SUIT B, SUIT C, SUIT state, Recommended, ...) of allowed values for the "MitoPedia concept" property. MitoFit Quality Control System"MitoFit Quality Control System" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
MitoPedia methods:
Respirometry,
Fluorometry,
Spectrophotometry
MitoPedia O2k and high-resolution respirometry:
DatLab