Difference between revisions of "SUIT-002 O2 ce-pce D007"
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::: '''[[Categories of SUIT protocols|SUIT-category]]:''' FNSGp(PGM) | ::: '''[[Categories of SUIT protocols|SUIT-category]]:''' FNSGp(PGM) | ||
::: '''[[SUIT protocol pattern]]:''' diametral | ::: '''[[SUIT protocol pattern]]:''' diametral | ||
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[[File:Reference protocol RP1&RP2_pce_PBMCs_PTLs.png|400px]] '''Harmonization between RP1 and RP2 in PBMC and PTL cells''' | [[File:Reference protocol RP1&RP2_pce_PBMCs_PTLs.png|400px]] '''Harmonization between RP1 and RP2 in PBMC and PTL cells''' | ||
== References == | == References == | ||
{{#ask:[[Category:Publications]] [[Additional label:: | {{#ask:[[Category:Publications]] [[Additional label::SUIT-2 O2 pce D07]] | ||
|?Was published in year=Year | | ?Was published in year=Year | ||
|?Has title=Reference | | ?Has title=Reference | ||
|?Mammal and model=Organism | | ?Mammal and model=Organism | ||
|?Tissue and cell=Tissue;cell | | ?Tissue and cell=Tissue;cell | ||
|format=broadtable | | format=broadtable | ||
|limit=5000 | | limit=5000 | ||
|offset=0 | | offset=0 | ||
|sort=Was published in year | | sort=Was published in year | ||
|order=descending | | order=descending | ||
}} | }} |
Revision as of 22:47, 17 July 2018
Description
Abbreviation: FNSGp(PGM)
Reference: A: SUIT 2 is the RP2 - MiPNet21.06 SUIT RP
SUIT number: D07_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
O2k-Application: O2
MitoPedia concepts:
SUIT protocol,
SUIT A
- MitoPedia: SUIT - SUIT reference protocol RP2
- SUIT-category: FNSGp(PGM)
- SUIT protocol pattern: diametral
Harmonized SUIT protocols
RP1 and RP2
- SUIT RP1: 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd
- SUIT RP2: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2
RP1 and RP2 in cells
RP2 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet Electron transfer-pathway statea.
- SUIT RP1 in cells: ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2 in cells: ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd
Harmonization between RP1 and RP2 in cells
RP1 and RP2 in PBMCs and PTLs
In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: SUIT 1 for PBMCs and PTLs
- SUIT RP1 in PBMCs and PTLsSUIT RP1 for PBMCs and PLTs:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd
- SUIT RP2 PBMCs and PLTs: 1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2 in PBMC and PTL cells