SUIT-001 O2 mt D001: Difference between revisions

Revision as of 13:56, 13 June 2018

high-resolution terminology - matching measurements at high-resolution

SUIT-001 O2 mt D001

Description

Abbreviation: FNSGp(PGM)

Reference: A: SUIT 1 is the RP1 - MiPNet21.06 SUIT RP

SUIT number: 1

MitoPedia concepts: SUIT protocol, SUIT A

MitoPedia: SUIT - SUIT reference protocol RP1

SUIT-category: FNSGp(PGM)

SUIT protocol pattern: orthogonal

Harmonized SUIT protocols

SUIT RP1 SUIT RP2

SUIT RP1:1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd

SUIT RP2:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

Harmonization between RP1 and RP2

RP1 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet Electron transfer-pathway states.

SUIT RP1 in cells:ce1;1Dig;1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd

SUIT RP2 in cells:ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd

Harmonization between RP1 and RP2 in cells

In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: SUIT 1 for PBMCs and PTLs

SUIT RP1 for PBMCs and PLTs:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd

SUIT RP2:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

Harmonization between RP1 and RP2 in PBMC and PTL cells

SUIT O2 DLP for SUIT 1 (RP1) (DatLab 7.3)

Updating this page (2018-06-13) for DatLab 7.3 DLP files

References

@@ Line 34: / Line 34: @@
-In PBMC and PLT the reference protocol RP1 is a different SUIT protocol (absense of Oct): [[1PM;2D;3U;4G;5S;6Rot;7Gp-|SUIT 3]]
+In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: [[1PM;2D;3U;4G;5S;6Rot;7Gp-|SUIT 1 for PBMCs and PTLs]]
+:::: '''SUIT RP1 for PBMCs and PLTs''':1PM;2D;2c;3U;4G;5S;6Rot;'''7Gp''';8Ama;'''9AsTm''';10Azd
+:::: [[1D;2M;3Oct;4M;5P;6G;7S;8Gp;9U;10Rot-|SUIT RP2]]:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd
-== SUIT O2 DLP for SUIT 1 (RP1) (DatLab 7.3) ==
-''Updating this page (2018-06-13) for DatLab 7.3''
+[[File:Reference protocol RP1&RP2_pce_PBMCs_PTLs.png|400px]] '''Harmonization between RP1 and RP2 in PBMC and PTL cells'''
-:::: [[DL-Protocols]] (DLP) for substrate-uncoupler-inhibitor titrations (see [[MitoPedia: SUIT]]) guide through a sequence of coupling and Electron transfer-pathway states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols [[MitoPedia: SUIT]]. Instrumental DL-protocols are displayed in the [[DL-Protocols#DL-Protocol_library| DL-Protocol library]].
-{{Template:Download instructions}}
+== SUIT O2 DLP for SUIT 1 (RP1) (DatLab 7.3) ==
-{| class="wikitable" border="1"
-|-
-! DL-Protocol
-! Description
-! Download - Protocol .DLP
-! Download - Analysis .XLSX
-|-
-| SUIT 1_pce
-| SUIT reference protocol 1 (RP1) for permeabilized cells (pce) with digitonin
-| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/0/05/RP1pce%28Dig%29.DLP]]
-| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/0/02/RP1pce%28Dig%29.xlsx]]
-|-
+''Updating this page (2018-06-13) for DatLab 7.3 DLP files''
-| SUIT 1_mt
-| SUIT reference protocol RP1 for mt-preparations: isolated mitochondria (imt) and tissue homogenate (thom)
-| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/a/a5/RP1mt.DLP]]
-| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/6/62/RP1mt.xlsx]]
-|-
-| SUIT 1_pfi
-| SUIT reference protocol RP1 for permeabilized fibers (pfi)
-| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/b/b9/RP1pfi.DLP]]
-| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/1/18/RP1pfi.xlsx]]
-|}