SUIT-001 O2 mt D001

high-resolution terminology - matching measurements at high-resolution

SUIT-001 O2 mt D001

Description

Abbreviation: FNSGp(PGM)

Reference: A: SUIT 1 is the RP1 - MiPNet21.06 SUIT RP

SUIT number: 1

MitoPedia concepts: SUIT protocol, SUIT A 

MitoPedia: SUIT - SUIT reference protocol RP1

SUIT-category: FNSGp(PGM)

SUIT protocol pattern: orthogonal

Harmonized SUIT protocols

SUIT RP1 SUIT RP2

SUIT RP1:1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd

SUIT RP2:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd

Harmonization between RP1 and RP2

RP1 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet Electron transfer-pathway states.

SUIT RP1 in cells:ce1;1Dig;1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd

SUIT RP2 in cells:ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd

Harmonization between RP1 and RP2 in cells

In PBMC and PLT the reference protocol RP1 is a different SUIT protocol (absense of Oct): SUIT 3

SUIT O2 DLP for SUIT 1 (RP1) (DatLab 7.3)

Updating this page (2018-06-13) for DatLab 7.3

DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and Electron transfer-pathway states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols MitoPedia: SUIT. Instrumental DL-protocols are displayed in the DL-Protocol library.

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DL-Protocol	Description	Download - Protocol .DLP	Download - Analysis .XLSX
SUIT 1_pce	SUIT reference protocol 1 (RP1) for permeabilized cells (pce) with digitonin
SUIT 1_mt	SUIT reference protocol RP1 for mt-preparations: isolated mitochondria (imt) and tissue homogenate (thom)
SUIT 1_pfi	SUIT reference protocol RP1 for permeabilized fibers (pfi)