O2k-OpenSupportAlert2020 Archive: Difference between revisions

Revision as of 10:05, 4 May 2020

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

May 2020

May-11 Th

Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.

- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis. - »Steady state«

May-04 Mo

Do you load the correct instrumental background correction accordingly to your range of experimental O₂ levels?

- See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«

Apr 2020

Apr-27 Mo

Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O₂ regime?

- To obtain an accurate O₂ background correction, you must select the marks that correspond to your range of experimental O₂ levels. - » O2 Background correction - troubleshooting«

Apr-20 Mo

Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?

- Unstable respiratory fluxes in OXPHOS might be due to substrates limitation. - » Pyruvate - troubleshooting«

Apr-14 Mo

Why are the oxygen levels different between chambers prior to my experimental run?

- After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations. - » Oxygen calibration - troubleshooting«

Apr-07 Mo

Did you know that you can edit the background parameters in the 'O₂ analysis template DL7.4?

- If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the O2 analysis template DL7.4 for completion of Oxygen flux analysis - DatLab 7.4. - »SUIT: Browse DL-Protocols and templates«

Apr-02 Mo

Did you know that you can edit the background parameters in the 'O₂ analysis template DL7.4?

- Do you want to search the best SUIT protocol for your research questions? - Use the SUITbrowser: »SUITbrowser«

Mar 2020

Mar-16 Mo

The standardized coupling control protocol to explore the bioenergetics of living cells

- DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »SUIT-003«

Mar-11 We

Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.

- »Blebbistatin«

Mar-06 Fr

Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.

- »RP1« and »RP2«

Mar-03 Tu

Rapamycin - more differences or more in common with other mitObesity drugs?

- »Rapamycin«

Feb 2020

Feb-26 We

It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.

- »Noncoupled respiration«

Feb-24 Mo

More research is needed on the impact of viral infection on mitochondrial function.

- »Viruses and mitochondrial medicine«

Feb-19 We

Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.

- »Spermidine«

Feb-18 Tu

Why do we fight gas bubbles?

- Dive deep into »Oxygen solubility«

Feb-12 We

What do metformin, melatonin and other bioactive mitObesity compounds have in common?

- »MitObesity drugs«

Jan 2020

Jan-16 Th

Oxygen flux

- which SI units should be used?

Did you ever think that units for body mass could be an issue?

- »Body mass«

Jan-08 We

Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.

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 * '''Apr-07 Mo'''
 :{|
-| width="100" align="center" | [[Image:Logo OROBOROS-DatLab.jpg|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]][[File:Blue-info.png|25px|Improve]]
+| width="100" align="center" | [[Image:Logo OROBOROS-DatLab.jpg|60px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]][[File:Blue-info.png|25px|Improve]]
 |'''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?'''
 - If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]].