- high-resolution terminology - matching measurements at high-resolution
NS-S pathway control efficiency
Description
The NS-S control factor (CI&II-CII substrate control factor) expresses the relative stimulation by N-linked substrates of S-linked respiration. In typical SUIT protocols with type N and S substrates, flux in the NS-linked substrate state, NS, is inhibited by Rotenone to measure flux in the S-linked substrate state, S. Then the NS-S control factor is
jNS-S = (NS-S)/NS
The NS-S control factor expresses the fractional change of flux in a defined coupling control state when inhibition by rotenone is removed from flux in the S-linked substrate state in the presence of a N-linked substrate combination. Experimentally rotenone (Rot) is added to the NS-linked state. The reversed protocol, adding N-linked substrates to a S-linked background state does not provide a valid estimation of S-linked respiration with succinate in the absence of Rot, since oxaloacetate accumulates as a potent inhibitor of succinate dehydrogenase (CII).
Abbreviation: jNS-S
Reference: Flux control factor
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
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