NS-S pathway control efficiency: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=''j''<sub> | |abbr=''j''<sub>NS-S</sub> | ||
|description=The '''CI<small>&</small>II-CII [[substrate control factor]]''' expresses the fractional change of flux in a defined [[coupling control state]] when inhibition by [[rotenone]] is removed from | |description=The '''NS-S control factor''' (CI<small>&</small>II-CII [[substrate control factor]]) expresses the relative stimulation by N-linked substrates of S-linked respiration. In typical [[SUIT protocol]]s with [[Substrate types |type N and S substrates]], flux in the [[NS-linked substrate state]], NS, is inhibited by [[Rotenone]] to measure flux in the [[S-linked substrate state]], S. Then the NS-S control factor is | ||
''j''<sub>NS-S</sub> = (NS-S)/NS | |||
The NS-S control factor expresses the fractional change of flux in a defined [[coupling control state]] when inhibition by [[rotenone]] is removed from flux in the S-linked substrate state in the presence of a N-linked substrate combination. Experimentally rotenone (Rot) is added to the NS-linked state. The reversed protocol, adding N-linked substrates to a S-linked background state does not provide a valid estimation of S-linked respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII). | |||
|info=[[Flux control factor]] | |info=[[Flux control factor]] | ||
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{{MitoPedia O2k and high-resolution respirometry}} | {{MitoPedia O2k and high-resolution respirometry}} | ||
== Compare == | == Compare == | ||
* [[CII control ratio]] | ::::* [[CII control ratio]] | ||
Revision as of 12:24, 20 March 2016
- high-resolution terminology - matching measurements at high-resolution
NS-S pathway control efficiency
Description
The NS-S control factor (CI&II-CII substrate control factor) expresses the relative stimulation by N-linked substrates of S-linked respiration. In typical SUIT protocols with type N and S substrates, flux in the NS-linked substrate state, NS, is inhibited by Rotenone to measure flux in the S-linked substrate state, S. Then the NS-S control factor is
jNS-S = (NS-S)/NS
The NS-S control factor expresses the fractional change of flux in a defined coupling control state when inhibition by rotenone is removed from flux in the S-linked substrate state in the presence of a N-linked substrate combination. Experimentally rotenone (Rot) is added to the NS-linked state. The reversed protocol, adding N-linked substrates to a S-linked background state does not provide a valid estimation of S-linked respiration with succinate in the absence of Rot, since oxaloacetate accumulates as a potent inhibitor of succinate dehydrogenase (CII).
Abbreviation: jNS-S
Reference: Flux control factor
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
Compare