Difference between revisions of "NS-S pathway control efficiency"
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|abbr=''j''<sub>NS-S</sub> | |abbr=''j''<sub>NS-S</sub> | ||
|description=The '''NS-S control factor''' (CI<small>&</small>II-CII [[substrate control factor]]) expresses the relative stimulation by N-linked substrates of S-linked respiration. In typical [[SUIT protocol]]s with [[ | |description=The '''NS-S control factor''' (CI<small>&</small>II-CII [[substrate control factor]]) expresses the relative stimulation by N-linked substrates of S-linked respiration. In typical [[SUIT protocol]]s with [[ETS substrate types |type N and S substrates]], flux in the [[NS-linked substrate state]], NS, is inhibited by [[Rotenone]] to measure flux in the [[S-linked substrate state]], S. Then the NS-S control factor is | ||
''j''<sub>NS-S</sub> = (NS-S)/NS | ''j''<sub>NS-S</sub> = (NS-S)/NS | ||
The NS-S control factor expresses the fractional change of flux in a defined [[coupling control state]] when inhibition by [[rotenone]] is removed from flux in the S-linked substrate state in the presence of a N-linked substrate combination. Experimentally rotenone (Rot) is added to the NS-linked state. The reversed protocol, adding N-linked substrates to a S-linked background state does not provide a valid estimation of S-linked respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII). | The NS-S control factor expresses the fractional change of flux in a defined [[coupling control state]] when inhibition by [[rotenone]] is removed from flux in the S-linked substrate state in the presence of a N-linked substrate combination. Experimentally rotenone (Rot) is added to the NS-linked state. The reversed protocol, adding N-linked substrates to a S-linked background state does not provide a valid estimation of S-linked respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate dehydrogenase]] (CII). | ||
Revision as of 20:28, 28 March 2016
- high-resolution terminology - matching measurements at high-resolution
NS-S pathway control efficiency
Description
The NS-S control factor (CI&II-CII substrate control factor) expresses the relative stimulation by N-linked substrates of S-linked respiration. In typical SUIT protocols with type N and S substrates, flux in the NS-linked substrate state, NS, is inhibited by Rotenone to measure flux in the S-linked substrate state, S. Then the NS-S control factor is
jNS-S = (NS-S)/NS
The NS-S control factor expresses the fractional change of flux in a defined coupling control state when inhibition by rotenone is removed from flux in the S-linked substrate state in the presence of a N-linked substrate combination. Experimentally rotenone (Rot) is added to the NS-linked state. The reversed protocol, adding N-linked substrates to a S-linked background state does not provide a valid estimation of S-linked respiration with succinate in the absence of Rot, since oxaloacetate accumulates as a potent inhibitor of succinate dehydrogenase (CII).
Abbreviation: jNS-S
Reference: Flux control factor
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
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