MiPNet21.06 SUIT RP
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OROBOROS (2016-01-19) Mitochondr Physiol Network
Abstract: Doerrier C, Sumbalova Z, Lamberti G, Krumschnabel G, Gnaiger (2016) SUIT reference assay for OXPHOS analysis by high-resolution respirometry. Mitochondr Physiol Network 21.06(pre03):1-10.
- » Instrument: O2k, O2k-Catalogue
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria
Regulation: Substrate Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-SOP
Laboratory sheets for SUIT protocols RP1 and RP2
SUIT reference assay for permeabilized fibres, Pfi
| Type | SUIT protocol | Sample | Abbr | pdf file | docx file |
|---|---|---|---|---|---|
| Titration sheet | RP1 | Permeabilized fibres | Pfi | »SUIT_RP1-Pfi | »SUIT_RP1-Pfi |
| Titration sheet | RP2 | Pfi | »SUIT_RP2-Pfi | »SUIT_RP2-Pfi | |
| Tick boxes | Combined | Pfi | »RP print-Pfi | »RP print-Pfi | |
| Titration sheet | RP1 | Permeabilized cells | Pc | »SUIT_RP1 | »SUIT_RP1 |
| Titration sheet | RP2 | Pc | »SUIT_RP2 | »SUIT_RP2 | |
| Tick boxes | Combined | Pc | »RP-Pc print | »RP-Pc print | |
| Titration sheet | RP1 | Isolated mitochondria, tissue homogenate | Imt, Thom | »SUIT_RP1 | »SUIT_RP1 |
| Titration sheet | RP2 | Imt, Thom | »SUIT_RP2 | »SUIT_RP2 | |
| Tick boxes | Combined | Imt, Thom | »RP print | »RP print |
Experimental details
Saturating ADP concentrations
Different concentrations of ADP are sufficient to obtain maximum flux for estimating OXPHOS capacity. The typical range for isolated mitochondria is 1 to 2.5 mM, for permeabilized cells 1 to 5 mM, for permeabilized muscle fibres 2.5 to 10 mM.
Pre-publication communication
This communication is a pre-publication, inviting critical feedback and comments. All feedback will be carefully documented and evaluated in terms of justification of co-authorship. We intend to finally publish this topic in a peer-reviewed journal as an original article, with reference to the pre-publication history including reviewer's reports. - Gnaiger Erich 03:23, 18 January 2016 (CET)
Further details
- Introduction » Gnaiger 2014 MitoPathways
- Table of titrations » MiPNet09.12 O2k-Titrations
- Definition » Substrate-uncoupler-inhibitor titration
- Context » SUIT protocol library
- Abbreviations » MitoPedia
