MiPNet21.06 SUIT RP

SUIT reference assay for OXPHOS analysis by high-resolution respirometry. - »Bioblast pdf«

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OROBOROS (2016-01-19) Mitochondr Physiol Network

Abstract: Doerrier C, Sumbalova Z, Lamberti G, Krumschnabel G, Gnaiger (2016) SUIT reference assay for OXPHOS analysis by high-resolution respirometry. Mitochondr Physiol Network 21.06(pre03):1-10.

» Instrument: O2k, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels: MiParea: Instruments;methods

Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria

Regulation: Substrate Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.

HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

O2k-SOP

Laboratory sheets for SUIT protocols RP1 and RP2

SUIT reference assay for permeabilized fibres, Pfi

Type	SUIT protocol	Sample	pdf file	docx file
Titration sheet	RP1	Pfi	»SUIT_RP1	»SUIT_RP1
Titration sheet	RP2	Pfi	»SUIT_RP2	»SUIT_RP2
Tick boxes	Combined	Pfi	»RP print	»RP print
Titration sheet	RP1	Pc,Imt	»SUIT_RP1	»SUIT_RP1
Titration sheet	RP2	Pc,Imt	»SUIT_RP2	»SUIT_RP2
Tick boxes	Combined	Pc,Imt	»RP print	»RP print

Pre-publication communication

This communication is a pre-publication, inviting critical feedback and comments. All feedback will be carefully documented and evaluated in terms of justification of co-authorship. We intend to finally publish this topic in a peer-reviewed journal as an original article, with reference to the pre-publication history including reviewer's reports. - Gnaiger Erich 03:23, 18 January 2016 (CET)

Further details

Introduction » Gnaiger 2014 MitoPathways
Table of titrations » MiPNet09.12 O2k-Titrations
Definition » Substrate-uncoupler-inhibitor titration
Context » SUIT protocol library
Abbreviations » MitoPedia