Kampa 2018 MiPschool Tromso C2
Cardioprotective flavoniods as a natural modulators of mitoBKCa channel. |
Link: MitoEAGLE
Kampa R, Kicinska A, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P (2018)
Event: MiPschool Tromso-Bergen 2018
Potassium channels such as KATP, BKCa or Kv1.3 have been found in the inner mitochondrial membranes of various cells. It is believed that potassium channels regulate the mitochondrial membrane potential, matrix volume, respiration, and Ca2+ ion homeostasis. There are hypotheses that mitochondrial BKCa channels play an important role in ischemic preconditioning. It was also shown that mitoBKCa channels are potential targets for some flavonoids in the anti-ischemic strategies.
Our pervious study, showed functional properties of the mitoBKCa channel in mitochondria of endothelial cells (EA.hy 926). Large conductance (270 pS), voltage dependence, a high open-state probability at positive potentials, sensitivity to Ca2+, NS1619 (a BKCa channel opener) and paxilline (BKCa channel inhibitor) indicate similarity to the mammalian BKCa channel. Previously, these channel was reported in glioma, skeletal muscle, brain and cardiac.
In the current study, single channel activity of the mitoBKCa channel was measured with patch-clamp technique of the mitoplasts isolated from EA.hy 926 endothelial cell line. We have shown data describing regulation of the mitoBKCa channel by different cardioprotective flavonoids (luteolin, quercitin and cyanidin). Cellular breathing tests using the oximeter were also carried out.
β’ Bioblast editor: Plangger M
Labels: MiParea: mt-Membrane
Organism: Human
Tissue;cell: Endothelial;epithelial;mesothelial cell
Enzyme: Inner mt-membrane transporter Regulation: Ion;substrate transport
Affiliations
Kampa R(1,2), Kicinska A(3), Kulawiak B(2), Jarmuszkiewicz W(3), Szewczyk A(2), Bednarczyk P(1)
- Dept Biophysics, Warsaw Univ Life Sciences
- Lab Intracellular Ion Channels, Nencki Inst Experimental Biol; Warsaw
- Lab Bioenergetics, Adam Mickiewicz Univ, Poznan; Poland
Support
This study was supported by a grant 2016/21/B/NZ1/02769 from the National Science Centre, Poland.