Carbonyl cyanide m-chlorophenyl hydrazone

high-resolution terminology - matching measurements at high-resolution

Carbonyl cyanide m-chlorophenyl hydrazone

Description

Carbonyl cyanide m-chloro phenyl hydrazone (C₉H₅ClN₄; FW = 204.62) is a protonophore (H⁺ ionophore) and chemical uncoupler of oxidative phosphorylation. CCCP causes uncoupling (uncoupler) of the proton gradient that is established during the normal activity of electron carriers in the electron transport system.

Abbreviation: CCCP

MitoPedia topics: Uncoupler 

Labels:

Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 

HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Application in HRR

C: CCCP (Carbonyl cyanide 3-chlorophenylhydrazone, C₉H₅ClN₄O); Sigma C 2759, 100 mg, store at -20 °C: FW = 204.62

Caution: Toxic!

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 1 mM stock solution (dissolved in DMSO):

1) weigh 1.02 mg of CCCP into a glass vial

2) dissolve in 5 ml DMSO

3) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution

3) store in glass vials at -20 °C

• Titration volume: 1 µl steps using a 10 µl syringe

• Final conc. in 2 ml O2k-chamber: 0.5 µM steps