MitoPediaAlert2020 Archive
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
June 2020
- Jun-08 Mo
Celebrating World Oceans Day
Would you like to measure the O2 flux that occurs during photosynthesis using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 NextGen-O2k project.
- »PhotoBiology«
- Jun-11 Th
Did you know that some chemicals are incompatible with the Amplex UltraRed assay?
- Jun-18 Th
Did you know the Crabtree effect can affect respiration in living cells? SUIT-003 is designed to analyze this important mechanism of respiratory control.
- »SUIT-003 O2 ce D037« and »SUIT-003 O2 ce D038«
- Jun-25 Th
Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?
- » Dithionite«
- Jul-02 Th
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:
- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity.Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«