From Bioblast
Description
Abbreviation: NS(GM)
Reference: A Β»Versions
- SUIT-category: NS(GM)
- SUIT protocol pattern: orthogonal 1GMS;2D;3Ama
Communicated by Komlodi T (last update 2019-01-22)
DLP applications
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
0DTPA |
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0SOD |
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0HRP |
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0AmR |
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Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
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- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- A succinate concentration > 10 mM might be required to reach saturating S-OXPHOS capacity.
- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.
- + Contribution of N pathway to the NS can be checked after the addition of Rot.
- + Reasonable duration of the experiment.
- + This protocol can be extended with the Complex IV module.
- - Rox may be lower in substrate states earlier in the SUIT protocol. Therefore, this Rox measurement is frequently taken as a methodological control rather than as the final basis of Rox correction of mitochondrial respiration (mt).
- - Careful washing is required after the experiment to avoid carry-over of inhibitors.
Compare SUIT protocols
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry,
Fluorometry