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Komlodi Timea

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BEC 2020.1 Mitochondrial physiology
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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

Komlodi Timea

MitoPedia topics: EAGLE 

COST: Member COST WG1: WG1



Name Komlodi Timea, PharmD,PhD ORCID ID
Department of Biochemistry Semmelweis University
Timea joined Oroboros Instruments from February 2017 to February 2022. She continues her collaboration with AT Innsbruck Oroboros from her position at the Semmelweis University in Budapest.
Address T眉zolto Strett 37-47, H - 1094
City Budapest
Country Hungary
Email [email protected]
O2k-Network Lab AT Innsbruck Oroboros, HU Budapest Tretter L
Template NextGen-O2k.jpg

Bioenergetics Communications

Keywords: mitochondria, bioenergetics, reactive oxygen species, coenzyme Q redox

MitoEAGLE Short-Term Scientific Mission

Motivation letter
I am writing this letter to apply for the Short-Term Scientific Mission (STSM) covered by MitoEAGLE鈥揅OST Action CA15203 (1st Call for STSM Applications).
My name is T铆mea Koml贸di. I studied at Semmelweis University at the Faculty of Pharmacy. After graduation, in 2013, I started working as a PhD student at the Semmelweis University in the Department of Medical Biochemistry, Hungary.
My main topic of interest is energy metabolism and mitochondrial homeostasis under physiological and pathological conditions. Our group focuses mainly on neurodegenerative diseases like Alzheimer鈥檚 and Parkinson鈥檚 disease, which are attributed to mitochondrial dysfunction. I have experience in measurement of mitochondrial respiration, ATP synthesis, membrane potential, reactive oxygen species production and intra- and extramitochondrial pH detection.
In the future I am planning to deepen my knowledge about mitochondria and to expand it towards cell lines. The STSM grant would give the opportunity to deal with mitochondrial physiology and cell lines at the Oroboros Instruments in Innsbruck. The focus of the STSM project is the standardization of protocols in mitochondrial respirometry which could contribute to the scientific objective of MitoEAGLE. During the 8-day STSM mission I could acquire new measurement protocols which could be applied in future projects. These are the planned program of the STSM mission: 1.) detection of reactive oxygen species production of permeabilized cells using fluorescent probes; 2.) determination of mitochondrial membrane potential with safranin, TMRM and TPP+; 3.) assessment of mitochondrial P/O ratio by measuring ATP levels fluorometrically.
The STSM training would provide a platform for an international discussion and standardization of protocols, data and measurement techniques. Furthermore, the STSM project could be a basis of a flourishing cooperation with the Oroboros laboratory in Innsbruck in the near future.
I would like to participate in the MitoEAGLE projects because it provides the opportunity to
take part in data establishment and comparison of data between different research groups.
Moreover, Oroboros laboratory would provide a platform with its instrumental background,with its protocols and with its international connections to the objectives and aim of MitoEAGLE.
STSM request: download pdf


        • Benefits for COST and for the COST Action MitoEAGLE
During my PhD I worked with brain, heart and liver mitochondria isolated from guinea pig and in this summer I started to deal with cultured cell lines

Participated at

Visiting scientist in the Oroboros O2k-Laboratory


Timea Koml貌di : Visiting scientist at the Oroboros O2k-Laboratory

  • 2016, November 21 to 28

Labels: Field of research: Basic 


Balmaceda 2024 Biochim Biophys Acta Mol Basis Dis2024Balmaceda V, Komlodi T, Szibor M, Gnaiger E, Moore AL, Fernandez-Vizarra E, Viscomi C (2024) The striking differences in the bioenergetics of brain and liver mitochondria are enhanced in mitochondrial disease. Biochim Biophys Acta Mol Basis Dis 1870:167033.
Ravasz 2024 Sci Rep2024Ravasz D, Bui D, Nazarian S, Pallag G, Karnok N, Roberts J, Marzullo BP, Tennant DA, Greenwood B, Kitayev A, Hill C, Koml贸di T, Doerrier C, Cunatova K, Fernandez-Vizarra E, Gnaiger E, Kiebish Michael A, Raska A, Kolev K, Czumbel B, Narain NR, Seyfried TN, Chinopoulos C (2024) Residual Complex I activity and amphidirectional Complex II operation support glutamate catabolism through mtSLP in anoxia. Sci Rep 14:1729.
Meszaros 2024 Transpl Int2024Meszaros AT, Weissenbacher A, Schartner M, Egelseer-Bruendl T, Hermann M, Unterweger J, Mittelberger C, Reyer BA, Hofmann J, Zelger BG, Hautz T, Resch T, Margreiter C, Maglione M, Koml贸di T, Ulmer H, Cardini B, Troppmair J, 脰fner D, Gnaiger E, Schneeberger S, Oberhuber R (2024) The predictive value of graft viability and bioenergetics testing towards the outcome in liver transplantation. Transpl Int 37.
Donnelly 2024 Redox Biol2024Donnelly C, Koml贸di T, Cecatto C, Cardoso LHD, Compagnion A-C, Matera A, Tavernari D, Campiche O, Paolicelli RC, Zanou N, Kayser B, Gnaiger E, Place N (2024) Functional hypoxia reduces mitochondrial calcium uptake. Redox Biol 71:103037.
Spielmann 2022 Mamm Genome2022Spielmann N, Schenkl C, Koml贸di T, da Silva-Buttkus P, Heyne E, Rohde J, Amarie OV, Rathkolb B, Gnaiger E, Doenst T, Fuchs H, Gailus-Durner V, de Angelis MH, Szibor M (2022) Knockout of the Complex III subunit Uqcrh causes bioenergetic impairment and cardiac contractile dysfunction. Mamm Genome 10.1007/s00335-022-09973-w
Fischer 2022 Metabolites2022Fischer C, Valente de Souza L, Koml贸di T, Garcia-Souza LF, Volani C, Tymoszuk P, Demetz E, Seifert M, Auer K, Hilbe R, Brigo N, Petzer V, Asshoff M, Gnaiger E, Weiss G (2022) Mitochondrial respiration in response to iron deficiency anemia. Comparison of peripheral blood mononuclear cells and liver.
Donnelly 2022 BEC2022Donnelly C, Schmitt S, Cecatto C, Cardoso LHD, Koml贸di T, Place N, Kayser B, Gnaiger E (2022) The ABC of hypoxia 鈥 what is the norm. Bioenerg Commun 2022.12.v2.
Komlodi 2022 BEC2022Koml贸di T, Tretter L (2022) The protonmotive force 鈥 not merely membrane potential. Bioenerg Commun 2022.16.
Pallag 2022 Int J Mol Sci2022Pallag G, Nazarian S, Ravasz D, Bui D, Koml贸di T, Doerrier C, Gnaiger E, Seyfried TN, Chinopoulos C (2022) Proline oxidation supports mitochondrial ATP production when Complex I is inhibited.
Horvath 2022 Antioxidants (Basel)2022Horv谩th G, Sv谩b G, Koml贸di T, Ravasz D, Kacs贸 G, Doczi J, Chinopoulos C, Ambrus A, Tretter L (2022) Reverse and forward electron flow-induced H2O2 formation is decreased in 伪-ketoglutarate dehydrogenase (伪-KGDH) subunit (E2 or E3) heterozygote knock out animals.
Komlodi 2021 BEC AmR-O22021Koml贸di T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4.
Komlodi 2021 BEC Q2021Koml贸di T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3.
Fischer 2021 Antioxidants2021Fischer C, Volani C, Koml贸di T, Seifert M, Demetz E, Valente de Souza L, Auer K, Petzer V, von Raffay L, Moser P, Gnaiger E, Weiss G (2021) Dietary iron overload and Hfe-/- related hemochromatosis alter hepatic mitochondrial function.
BEC 2020.1 doi10.26124bec2020-0001.v12020Gnaiger E et al 鈥 MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1.
Mikulas 2020 Materials2020Mikul谩s K, Koml贸di T, F枚ldes A, Sv谩b G, Horv谩th G, Nagy AM, Ambrus A, Gyulai-Ga谩l Sz, Gera I, Hermann P, Varga G, Tretter L (2020) Bioenergetic Impairment of triethylene glycol dimethacrylate- (TEGDMA-) treated dental pulp stem cells (DPSCs) and isolated brain mitochondria are amended by redox compound methylene blue. Materials (Basel) 13(16):3472.
Komlodi 2018 J Bioenerg Biomembr2018Koml贸di T, Geibl FF, Sassani M, Ambrus A, Tretter L (2018) Membrane potential and delta pH dependency of reverse electron transport-associated hydrogen peroxide production in brain and heart mitochondria. J Bioenerg Biomembr 50:355-365
Mikulas 2018 Dent Mater2018Mikul谩s K, Hermann P, Gera I, Koml贸di T, Horv谩th G, Ambrus A, Tretter L (2018) Triethylene glycol dimethacrylate impairs bioenergetic functions and induces oxidative stress in mitochondria via inhibiting respiratory Complex I. Dent Mater 34:e166-e181.
Komlodi 2018 Methods Mol Biol2018Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.
Komlodi 2017 Neuropharmacology2017Koml贸di T, Tretter L (2017) Methylene blue stimulates substrate-level phosphorylation catalysed by succinyl-CoA ligase in the citric acid cycle. Neuropharmacology 123:287-98.
Koncsos 2016 Am J Physiol Heart Circ Physiol2016Koncsos G, Varga ZV, Baranyai T, Boengler K, Rohrbach S, Li L, Schl眉ter KD, Schreckenberg R, Radovits T, Ol谩h A, M谩ty谩s C, Lux 脕, Al-Khrasani M, Koml贸di T, Bukosza N, M谩th茅 D, Deres L, Bartekov谩 M, Rajt铆k T, Adameov谩 A, Szigeti K, Hamar P, Helyes Z, Tretter L, Pacher P, Merkely B, Giricz Z, Schulz R, Ferdinandy P (2016) Diastolic dysfunction in prediabetic male rats: role of mitochondrial oxidative stress. Am J Physiol Heart Circ Physiol 311:H927-43.


Donnelly 2024 EU-METAHEART MC2 Meeting2024Donnelly C, Koml贸di T, Cecatto C, Cardoso LHD, Kayser B, Place N, Gnaiger E (2024) Functional hypoxia in cardiac mitochondria: effects on oxidative phosphorylation, mitochondrial membrane potential, redox state of coenzyme Q, and calcium uptake. EU-METAHEART MC2 Meeting 2024 Antalya TR.
Komlodi 2023 EUROMIT2023 Bologna2023Koml贸di T, Silva FSG, Duarte AI, Mena D, Garcia-Souza LF, Makrecka-Kuka M, Bento G, Grilo LF, Oliveira PJ, Gnaiger E (2023) Off-target effects of etomoxir: inhibition of mitochondrial Complex I and fatty acid oxidation. EUROMIT2023 International Conference on Mitochondrial Pathology.
Schmitt 2023 BPS2023 San Diego2023
Erich Gnaiger
Schmitt S, Cardoso LHD, Cecatto C, Komlodi T, Gnaiger Erich (2023) Mitochondrial bioenergetics 鈥 a quantitative analytical and diagnostic approach. 67th Annual Meeting of the Biophysical Society.
Pallag 2022 Abstract Bioblast2022P06.
Pallag Gergely
Pallag Gergely, Nazarian S, Ravasz D, Bui D, Koml贸di T, Doerrier C, Gnaiger E, Seyfried TN, Chinopoulos C (2022) Proline oxidation supports mitochondrial ATP production when Complex I is inhibited. Bioblast 2022: BEC Inaugural Conference. In:
MitoFit Preprint
Donnelly 2022 Abstract Bioblast20222.1. 芦10+5禄
Donnelly Chris
Donnelly Chris, Schmitt S, Cecatto C, Cardoso L, Komlodi T, Place N, Kayser B, Gnaiger E (2022) The ABC of hypoxia 鈥 what is the norm. Bioblast 2022: BEC Inaugural Conference. In:
MitoFit Preprint 禄Watch the presentation芦
Cardoso 2022 Q10 Hamburg2022Cardoso LHD, Donnelly C, Komlodi T, Gnaiger E (2022) Coenzyme Q redox state and respiration in permeabilized HEK 293T cells: coupling and pathway control. Q10 Hamburg.
Komlodi 2022 Abstract Bioblast20223.1. 芦10+5禄
Komlodi Timea
Koml贸di Timea, Tretter L (2022) The protonmotive force 鈥 not merely membrane potential. Bioblast 2022: BEC Inaugural Conference. In:
MitoFit Preprint 禄Watch the presentation芦
Komlodi 2022 Q10 Hamburg2022Komlodi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2022) The mitochondrial Q-junction and coenzyme Q pools: Continuous monitoring of pull and push control of respiration and redox state of the Q-mimetic CoQ2. Q10 Hamburg.
Cardoso 2022 ESCI Bari2022
Luiza Cardoso
Cardoso LHD, Donnelly C, Koml贸di T, Gnaiger E (2022) Bioenergetic fingerprinting with coupling and pathway control of coenzyme Q redox state and respiration in permeabilized HEK 293T cells. 56th Annual Scientific Meeting of the European Society for Clinical Investigation.
Garcia-Roves 2022 Abstract Bioblast20224.4. 芦10+5禄
Pablo Garcia-Roves
Gama-Perez P, Cardoso L, Komlodi K, Bosch N, Gnaiger E, Garcia-Roves Pablo M (2022) Tuning the assessment of coenzyme Q redox state and respiration in permeabilized skeletal muscle fibers. Bioblast 2022: BEC Inaugural Conference. In: 禄Watch the presentation芦
Cardoso 2022a EBEC2022
Luiza Cardoso
Cardoso LHD, Donnelly C, Koml贸di T, Gnaiger E (2022) Characterizing electron transfer through the mitochondrial Q-junction from fatty acid oxidation and TCA cycle on HEK 293T cells: correlation with respiration. EBEC 2022.
Cardoso 2022 Abstract Bioblast20224.3. 芦10+5禄
Luiza Cardoso
Cardoso Luiza HD, Doerrier C, Donnelly C, Koml贸di T, Gnaiger E (2022) Redox monitoring and respiration - a new horizon with the NextGen-O2k. Bioblast 2022: BEC Inaugural Conference. In: 禄Watch the presentation芦
Ravasz 2019 Abstract IOC1412019Ravasz D, Bui D, Kitayev A, Greenwood B, Hill C, Komlodi T, Doerrier C, Ozohanics O, Moore AL, Gnaiger E, Kiebish M, Kolev K, Seyfried TN, Willis WT, Narain N, Adam-Vizi V, Chinopoulos C (2019) Endogenous quinones sustain a moderate NADH oxidation by Complex I during anoxia. Mitochondr Physiol Network 24.02.
Silva 2019 ESCI20192019Silva FSG, Komlodi T, Garcia-Souza LF, Bento G, Doerrier C, Oliveira PJ, Gnaiger E (2019) Can fatty acid oxidation be specifically blocked by the CPT1 inhibitor etomoxir?
Silva 2019 MiPschool Coimbra2019
Filomena Pereira da Silva Grilo da Silva
Off-target effects of etomoxir on mitochondrial Complex I.
Komlodi 2018 EBEC20182018Electron supply to the Q-junction: assessment of mitochondrial respiration, H2O2 flux and the redox state of the Q-pool.
Gnaiger 2018 AussieMit2018Komlodi Timea, Sobotka Ondrej, Doerrier Carolina, Gnaiger Erich (2018) Mitochondrial H2O2 production is low under tissue normoxia but high at in-vitro air-level oxygen pressure - comparison of LEAK and OXPHOS states. AussieMit 2018 Melbourne AU.
Pereira da Silva Grilo da Silva 2018 MiP20182018
Filomena Pereira da Silva Grilo da Silva
The unspecific effect of etomoxir on mitochondrial respiration.
Komlodi 2018 AussieMit2018Komlodi T, Hunger M, Moore AL, Gnaiger E (2018) Electron transfer at the Q-junction: new perspectives from combined measurement of mitochondrial O2 flux, H2O2 flux, and coenzyme Q redox state. AussieMit 2018 Melbourne AU.
Komlodi 2018b EBEC20182018Endogenous quinones sustain NADH oxidation by Complex I during anoxia, supporting substrate-level phosphorylation in mouse liver mitochondria.
Huete-Ortega 2018 AlgaEurope20182018Huete-Ortega M, Komlodi T, Pranger F, Gnaiger E (2018) High-resolution respirometry: new perspectives for the study of microalgae bioenergetics and applications in biotechnology. AlgaEurope2018.
Sobotka 2018 MiP20182018
Ondrej Sobotka
Measurement of ROS production under hypoxia and unexpected methodological pitfalls of Amplex UltraRed assay.
Huete-Ortega 2018 MiPschool Tromso E22018High-resolution respirometry: new perspectives for the study of bioenergetics in algae and plants.
Chang 2018 Life Sciences Meeting 2018 Innsbruck AT2018pH dependence of mitochondrial respiration and H2O2 production in oral cancer cells 鈥 a pilot study.
Ravasz 2018 Abstract The evolving concept of mitochondria2018Vast pools of endogenous quinones sustain NADH oxidation by Complex I during anoxia, supporting substrate-level phosphorylation in mouse liver mitochondria.
Komlodi 2017 Abstract MITOEAGLE Barcelona2017
Succinate dehydrogenase regulation via oxaloacetate in brain mitochondria.
Sobotka 2017 MiPschool Obergurgl2017
Ondrej Sobotka
Reference protocols for ROS production measurements.
Komlodi 2017 MiPschool Obergurgl2017
Timea Komlodi
Electron pressure exerted by convergent succinate- and glycerophosphate-pathways to the Q-junction regulate reversed electron transfer to Complex I and H2O2 production.
Komlodi 2017 MiP20172017
Timea Komlodi
H2O2 production under hypoxia in brain and heart mitochondria: does O2 concentration matter?
Komlodi 2016 Abstract IOC1162016Komlodi T, Geibl F, Sassani M, Adam-Vizi V, Tretter L (2016) The effect of 螖mt and 螖pH on mitochondrial reactive oxygen species production - A comparative study using mitochondria isolated from different mammalian tissues. Mitochondr Physiol Network 21.11
Meszaros 2014 Abstract MiP20142014Methodological aspects of assessing mitochondrial function in small intestinal mucosal samples of rats and guinea pigs.
Komlodi 2014 Abstract MiP20142014Methylenblue, an enhancer of mitochondrial substrate-level phosphorylation.
Tretter 2013 Abstract MiP20132013Tretter L, Koml贸di T, 脕d谩m-Vizi V (2013) The effects of methylmalonic acid on alpha-ketoglutarate supported oxidation in isolated brain, heart and liver mitochondria. Mitochondr Physiol Network 18.08.