Template talk:Next oroboros ecosystem agenda: Difference between revisions
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::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases. | ::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases. | ||
* '''Jun-29 Mo''' | |||
:{| | |||
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] | |||
|'''Find out how to evaluate the effect of the obtained ‘O2 slope neg’ noise on experimental results'''<br> | |||
- » [[Oxygen_sensor_test#Figure_2._POS_Quality_control_using_the_DatLab_protocol_.28MiPNet06.03_POS-calibration-SOP.29| QC1: Oxygen sensor test]]« | |||
|} | |||
or | |||
:{| | |||
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] | |||
|'''How to decide whether the noise of the O2 slope neg. is within recommended values?'''<br> | |||
- » [[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Noise of 'O2 slope neg.']]« | |||
|} | |||
(NOTE for 4-eyes: [[Oxygen_sensor_test#Figure_2._POS_Quality_control_using_the_DatLab_protocol_.28MiPNet06.03_POS-calibration-SOP.29| QC1: Oxygen sensor test]] is the O2k-Support page for sensor test, and [[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Noise of 'O2 slope neg.']] is the updated version by Erich that belongs to MitoPedia:_DatLab#Calibration. What to do with this pages? Shall the info remain in those locations or shall be linked instead?) | |||
=== Removed for correction === | === Removed for correction === | ||
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An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br> | An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br> | ||
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly« | - »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly« | ||
|} | |} | ||
Revision as of 11:23, 16 June 2020
- Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
{| | logos | title in bold <br> - link |}
e.g.,
Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?
- »Instrumental background« and »TIP2k Filter Papers«
- See the scheme:
Bioblast alert 2020
Prep for circular done here: Talk:Bioblast alert 2020
MitoPedia alert
Next
- Jun-25 Th
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells, or in mitochondrial preparations, then you face an experimental artefact? The reasons which may trigger this experimental artefact are: - loss of oxidative capacity during the time course of the respirometric assay, |
or
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells, or in mitochondrial preparations, then you face an experimental artefact? The reasons which may trigger this experimental artefact are: - » Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 doi:10.26124/bec:2020-0001.v1« |
- Jul-02 Th
Did you know that Dithionite should not be used to control the oxygen concentration in experiments with biological samples?
- » Dithionite«
or
Do you know the different possibilities to adjust oxygen concentration in the Oroboros O2k?
(NOTE for 4-eyes: the page https://wiki.oroboros.at/index.php/Setting_the_oxygen_concentration is currently under editions. See the talk page: https://wiki.oroboros.at/index.php/Talk:Setting_the_oxygen_concentration)
O2k-Brief alert
Next
O2k-Publications alert
Next
- Jun-23 Tu
ASCs Exosomes: a potential therapeutic strategy to restore mitochondrial function in ALS |
O2k-Open Support alert
Next
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
- Jun-29 Mo
or
(NOTE for 4-eyes: QC1: Oxygen sensor test is the O2k-Support page for sensor test, and Noise of 'O2 slope neg.' is the updated version by Erich that belongs to MitoPedia:_DatLab#Calibration. What to do with this pages? Shall the info remain in those locations or shall be linked instead?)
Removed for correction
Do you perform the leaky chamber test after chamber assembly?
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«
O2k-Open Support alert circulars
O2k-Open Support alert circular 2020(01): in prep
New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
See how you can check that the two blue OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«
New Calibration parameters are transferred to experimental DatLab files
Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files.
- » Oxygen calibration - DatLab«
Do you perform O2k-Chamber volume calibration?
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module).
- »O2k-Chamber MitoPedia«
Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
The O2 solubility factor varies according to the medium used and influences the air calibration.
- »Oxygen solubility factor«
In preparation
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: - Proven -80 °C solid stability for more than 3 years (3 years 7 months)
- Proven -20 °C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 °C for 6 months (ongoing)
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1