MitoPediaAlert2020 Archive: Difference between revisions
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:::::: <big>''Currently Answered Questions from the Β»[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]Β« | :::::: <big>''Currently Answered Questions from the Β»[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]Β« | ||
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== June 2020 == | |||
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* '''Jun-04 Th''' | |||
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| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | |||
| '''Does your sample display a preference for [[GM|glutamate & malate]] over [[PM|pyruvate & malate]] to support NADH-linked respiration?''' <br> | |||
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallelΒ to evaluate the contribution of different combinations of two NADH-linked respiration substrates. <br> | |||
-Β»'''[[SUIT-008]]'''Β« and Β»'''[[SUIT-014]]'''Β« | |||
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== Previous MitoPedia Alert 2020 == | |||
[[File:Expand.png|right|35px |Click to expand or collaps]] | |||
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<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span> | |||
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== May 2020 == | == May 2020 == | ||
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- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - Β»'''[[SUIT-002 |SUIT-002]]'''Β« | - Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - Β»'''[[SUIT-002 |SUIT-002]]'''Β« | ||
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=== Apr 2020 === | === Apr 2020 === | ||
Revision as of 12:26, 29 May 2020
- Currently Answered Questions from the Β»Oroboros Ecosystem AgendaΒ«
June 2020
- Jun-04 Th
Does your sample display a preference for glutamate & malate over pyruvate & malate to support NADH-linked respiration?
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates.
-Β»SUIT-008Β« and Β»SUIT-014Β«
Previous MitoPedia Alert 2020
>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
May 2020
- May-28 Th
Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas?
- Β»Nitrogen injectionΒ«
- May-21 Th
As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production.
-Β»PhotoBiologyΒ«
- May-14 Th
How can I measure ATP production with the O2k?
Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green
- Β»Magnesium GreenΒ«
- May-07 Th
Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it? Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- Learn more about - Β»malate anaplerotic pathway control stateΒ«
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - Β»SUIT-027Β«
- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - Β»SUIT-002Β«
Apr 2020
- Apr-24 Fr
ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.
- Apr-21 Tu
Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Apr-13 Mo
Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation
- Β»SUIT-024Β«
- Apr-08 We
The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration.
- Β»SUIT-010Β«
- Apr-02 Th
Do you want to search the best SUIT protocol for your research questions?
- Use the SUITbrowser: Β»SUITbrowserΒ«
Mar 2020
- Mar-16 Mo
The standardized coupling control protocol to explore the bioenergetics of living cells
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions.
- Β»SUIT-003Β«
- Mar-11 We
Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.
- Β»BlebbistatinΒ«
- Mar-06 Fr
Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.
- Mar-03 Tu
Rapamycin - more differences or more in common with other mitObesity drugs?
- Β»RapamycinΒ«
Feb 2020
- Feb-26 We
It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.
- Β»Noncoupled respirationΒ«
- Feb-24 Mo
- Feb-19 We
Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.
- Β»SpermidineΒ«
- Feb-18 Tu
Why do we fight gas bubbles?
- Dive deep into Β»Oxygen solubilityΒ«
- Feb-12 We
What do metformin, melatonin and other bioactive mitObesity compounds have in common?
- Β»MitObesity drugsΒ«
Jan 2020
- Jan-16 Th
Oxygen flux
- which SI units should be used?
Did you ever think that units for body mass could be an issue?
- Β»Body massΒ«
- Jan-08 We