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From Bioblast
| Experience with FAO in PFI | Fatty acid used | Mammal and model | Nonmammal | Tissue and cell | Cell line | |
|---|---|---|---|---|---|---|
| Schoettl 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | Beta oxidation in adipocytes (white and brown), HEK293 cells, isolated mitochondria from liver, adipocytes, brain, etc. | Palmitoylcarnitine | Nervous system Liver Fat HEK | |||
| Senyilmaz 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | Inverted Drosophila larvae which are permeabilized by digitonin. Their body walls are more or less made of muscles. | Palmitoyl-CoA + Carnitine | Drosophila | Skeletal muscle | ||
| Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | I have extensively tested FAO in PMFBs using the Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine. Protocol: For maximal respiration we make sequential additions of 18 µM PC, 20 µM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition. | Palmitoylcarnitine Palmitoyl-CoA and L-Carnitine | ||||
| Sparks 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | We routinely (up to 4 human and mouse studies) measure respiration supported by glycolytic and fatty acid substrates using SUIT protocols in permeabilized muscle fibers. We always use palmitoylcarnitine. In the past, in Dr. Schrauwen’s lab, we used octanoate to titrate for fatty acid induced uncoupling. | Palmitoylcarnitine Octanoate | Human Mouse | Skeletal muscle | ||
| Trifunovic 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | Palmitoylcarnitine works better than Octanoylcarnitine. | Octanoylcarnitine Palmitoylcarnitine | Heart | |||
| Van Schaardenburgh 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | In my studies on patients with intermittent claudication we used Octanoylcarnitine in the SUIT protocol described by Pesta and you. | Octanoylcarnitine | ||||
| Veksler 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | We studied fatty acid oxidation in permeabilised cardiac and skeletal muscle fibers for 15 years. We use palmitoylcarnitine, palmitoyl-CoA or octanoate | Palmitoylcarnitine Palmitoyl-CoA Octanoate | Heart Skeletal muscle | |||
| Villena 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | C14-labelled palmitic acid | Fat | ||||
| Votion 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | Very limited (only preliminary tests on mice) but satisfying. I have planned new experiments on equine muscle tissue in 2016. | Octanoylcarnitine Palmitoylcarnitine Octanoate Acetylcarnitine Palmitoyl CoA (+ Carnitine) | Mouse | |||
| Weber 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | Octanoylcarnitine | Human Mouse | Genital | |||
| West 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | Octanoylcarnitine | |||||
| Wohlwend 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | Fatty acid oxidation in permeabilized muscle fibers has worked well for us in both human and rat studies. Typically we use Octanoylcarnitine and Malate for skeletal muscle and a combination of Octanoylcarnitine plus Palmitylcarnitine and Malate for human heart tissue. These protocols have given us a good insight into Beta oxidation. | Octanoylcarnitine + Malate Octanoylcarnitine + Palmitoylcarnitine + Malate (for heart) | Human Rat | Skeletal muscle | ||
| Wright 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | We do include octanoylcarnitine in the initial titration of substrates for our SUIT protocols typically, but do not measure its individual contribution to OXPHOS. | Octanoylcarnitine | ||||
| Wuest 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | Compared with just glucose oxidation, the max OXPHOS/ET capacity was slightly (but significantly) lower with PC & Oct. Protocol: PC, M, ADP, OCT, G, P, SUC, (FCCP, rat), ROT, AA. The concentrations were the same as in the blue book. | Palmitoylcarnitine + Octanoylcarnitine | Rat | Heart |
