Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

From Bioblast
Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

Link: O2k-Network Discussion Forum

Smith CD (2015)

Event: Fatty Acid Oxidation O2k-Network Discussion Forum

I have extensively tested FAO in PMFBs using the Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine.

Protocol: For maximal respiration we make sequential additions of 18 ยตM PC, 20 ยตM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition.


Labels:



Preparation: Permeabilized tissue 


Coupling state: OXPHOS, ET  Pathway:


Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine