Difference between revisions of "Ziegler 1962 Arch Biochem Biophys"
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{{Publication | {{Publication | ||
|title=Ziegler DM, Doeg KA (1962) Studies on the electron transport system XLIII. The isolation of a succinic-coenzyme Q reductase from beef heart mitochondria. Arch Biochem Biophys 97: 41-50. | |title=Ziegler DM, Doeg KA (1962) Studies on the electron transport system XLIII. The isolation of a succinic-coenzyme Q reductase from beef heart mitochondria. Arch Biochem Biophys 97:41-50. | ||
|info=[http://www.sciencedirect.com/science/journal/00039861/97/1 ScienceDirect] | |info=[http://www.sciencedirect.com/science/journal/00039861/97/1 ScienceDirect] | ||
|authors=Ziegler DM, Doeg KA | |authors=Ziegler DM, Doeg KA | ||
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|organism=Bovines | |organism=Bovines | ||
|tissues=Heart | |tissues=Heart | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|enzymes=Complex II; | |enzymes=Complex II;succinate dehydrogenase | ||
|additional=Made history | |additional=Made history | ||
}} | }} |
Latest revision as of 11:04, 13 February 2015
Ziegler DM, Doeg KA (1962) Studies on the electron transport system XLIII. The isolation of a succinic-coenzyme Q reductase from beef heart mitochondria. Arch Biochem Biophys 97:41-50. |
Ziegler DM, Doeg KA (1962) Arch Biochem Biophys
Abstract: The isolation of the succinic-CoQ reductase from beef heart mitochondria is described. The flavine content of the preparation is 4.8 mฮผmoles/mg. protein, and all of the flavine is extracted by acid only after the preparation is treated with proteolytic enzymes. The preparation also contains non-heme iron, lipid, and protoheme, and the last mentioned is present in an amount equivalent to the flavine. Based on the flavine or heme content, the minimum molecular weight in terms of protein is 210,000. The heme present in the purified enzyme is not reduced by succinate, which makes its participation as an electron carrier in the reactions catalyzed by the enzyme very unlikely.
A rapid spectrophotometric method for measuring the succinic-CoQ reductase activity of mitochondria and of the purified enzyme is described. โข Keywords: Succinic-coenzyme Q reductase, Flavine content, Beef heart mitochondria
Labels:
Organism: Bovines
Tissue;cell: Heart
Preparation: Isolated mitochondria
Enzyme: Complex II;succinate dehydrogenase
Made history