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|title=Grefte S, Wagenaars JA, Jansen R, Willems PH, Koopman WJ (2015) Rotenone inhibits pr |authors=Grefte S, Wagenaars JA, Jansen R, Willems PH, Koopman WJ

|abbr=''j''_NS-S ...measure flux in the [[S-pathway control state]], S(Rot) or S. Then the NS-S pathway control efficiency in the ET-coupling state is

|abbr=S/NS ...valid estimation of S-linked respiration with succinate in the absence of Rot, since [[oxaloacetate]] accumulates as a potent inhibitor of [[succinate de

|title=Zeng R, Zhou Q, Zhang W, Fu X, Wu Q, Lu Y, Shi J, Zhou S (2019) Icariin-mediated activation of autophagy confers protective effect o |authors=Zeng R, Zhou Q, Zhang W, Fu X, Wu Q, Lu Y, Shi J, Zhou S

...binations in SUIT protocols, particularly: ([[N-pathway control state]], [[S-pathway control state]], [[NS-pathway control state]]). Titrations may perf :::* S: S(Rot), S, SM, ..

|title=Zeng R, Wang X, Zhou Q, Fu X, Wu Q, Lu Y, Shi J, Klaunig JE, Zhou S (2019) Icariin protects rotenone-induced neurotoxicity through induction of |authors=Zeng R, Wang X*, Zhou Q, Fu X, Wu Q, Lu Y, Shi J, Klaunig JE, Zhou S

#P_M+Palmitoylcarnitine+S #E_S+Rot

...ate establishes the concept that electron pressure generated by the linear S- and Gp-pathways or the convergent SGp-pathway on the Q-junction drives RET |pathways=N, S, Gp, Other combinations, ROX

|info=[[File:Grefte_S.jpg|150px|right|Grefte S]] [[Laner 2014 Mitochondr Physiol Network MiP2014|Mitochondr Physiol Networ |authors=Grefte S, Wagenaars JA, Jansen R, Willems PH, Koopman WJ

...succinate (10 mM) alone, S, or succinate (10 mM) with rotenone (0.5 μM), S(Rot), on mitochondrial respiration in cardiac mitochondria isolated from wild-t ...addition of ADP was diametrically different for S (decrease of NADH) and S(Rot) (increase of NADH).

'''Protocol''': PC, M, ADP, OCT, G, P, SUC, (FCCP, rat), ROT, AA. The concentrations were the same as in the blue book. |pathways=F, N, S, NS, ROX

:::* It is a protocol to investigate the S- and NS-pathway linked respiration. ::: + Cytochrome ''c'' test can be performed in the S-OXPHOS state.

...succinate (10 mM) alone, S, or succinate (10 mM) with rotenone (0.5 μM), S(Rot), on mitochondrial respiration, hydrogen peroxide (H₂O<sub>2</su ..., mitochondrial respiration increased by 70%. In contrast ADP titration to S induced a decline in respiration by 30% with respect to the LEAK state, whi

|abbr=S, SRot ...nked respiration; SRot; see [[Gnaiger 2009 Int J Biochem Cell Biol]]). The S-pathway describes the electron flux through [[Complex II]] (CII; see [[succ

|abbr=PM+S+Rot ...te| NS-pathway]] and [[S-pathway control state]] in the OXPHOS state and [[S-pathway control state]] in the ET state in isolated mitochondria and permea

...n of ADP was diametrically different for S(-Rot) (decrease of NADH) and S(+Rot) (increase of NADH) in mouse cardiac tissue. ...ly produced oxaloacetate (Oa), which is particulaly pronounced in state S(-Rot) [4]. However, exogenously added Oa inhibited SDH measured in brain and kid

|abbr= CCP mt S(Rot) ...chondrial O₂ consumption and the redox state of the Q-pool in S(Rot)-pathway control state in isolated mitochondria- '''[[SUIT-006]]'''

...th OM and heart mitochondria. In contrast, with complex II substrate SUC + ROT, only inhibitory effects on mitochondrial respiration was observed in both {{Template:Correction FADH2 and S-pathway}}

|abbr=CCP ce-pce S(Rot) ...chondrial O₂ consumption and the redox state of the Q-pool in S(Rot)-pathway control state in permeabilized cells- '''[[SUIT-006]]'''

|title=Stadlmann S, Renner K, Pollheimer J, Moser PL, Zeimet AG, Offner FA, Gnaiger E (2006) P |authors=Stadlmann S, Renner K, Pollheimer J, Moser PL, Zeimet AG, Offner FA, Gnaiger Erich

|abbr=Rot |abbr=Rot

:::* It is a protocol to analyse the S- and NS-pathway linked respiration. :::+ Cytochrome ''c'' test can be performed in the S-OXPHOS state.

|abbr= PM+S+Rot ...extended coupling-control proton in the N-pathway and contribution of G-, S-, Oct- and Gp-pathways in the ET state for isolated mitochondria, tissue ho

|abbr= PM+S+Rot ...extended coupling-control proton in the N-pathway and contribution of G-, S-, Oct- and Gp-pathways in the ET state for isolated mitochondria, tissue ho

|abbr= PM+S+Rot |info='''A''' to analyse O2 flux and the Q-redox state in the N-,NS- and S- pathway control state on isolated mitochondria- '''[[SUIT-031]]'''

|abbr=CCP mt S(Rot) ...hibits complex I, provides the best coupling-control protocol specific for S-pathway, avoiding reverse electron transfer (RET).

...as well as of the NADH-linked and succinate (S)-pathways separately (F, N, S) and in combination (FN, FS, FNS, NS). ...gests an unspecific effect of on mitochondrial respiration involving N-and S-pathways and raises caution in its application as specific inhibitor of FAO

|abbr=OXPHOS (F+M+P+G+S+Rot) :::+ Pathway control in OXPHOS (F, F(N), FN, FNS and S pathways) can be evaluated.

...ion, ROS, Reactive oxygen species, ROT, Rotenone, Reactive oxygen species, S, Succinate, TMPD, N,N,N’,N’-tetramethyl-p-phenylenediamine, dph, Days p |pathways=N, S, CIV, NS, ROX

|abbr=QC_imt_PM_T+OXPHOS+c+Omy_ET_G+S+Rot ...rment N-pathway capacity can be compensated partially by activation of the S-pathway.

...| '''M''' || '''Dig''' || '''ADP''' || '''G''' || '''S''' || '''u''' || '''Rot''' || '''Ama''' ...''u''': uncoupler (CCCP: carbonyl cyanide m-chloro phenyl hydrazone); '''Rot''': Rotenone; '''Ama''': Antimycin A.

|abbr= PM+S+Rot ...strates (PM) and the contribution of the S-pathway to the ET state (N, NS, S).

...Oroboros Oxygraph-2k chambers with appropriate SUIT protocol (GMP+D+c+Rot+S+Ama+As+Tm+Azd) in mitochondrial respiratory medium MIR06 [3]. Oxygen concen |pathways=N, S, NS

...CII/CI&II flux ratios of 0.8 (0.6) for OXPHOS (or ETS). The GMP/GMSE and S(Rot)E/GMSE flux control ratios are 0.50 and 0.55 in human vastus lateralis (Pes

...mplementary to [[pathway control state]]s (PCS), [[coupling control state]]s (CCS: ET, OXPHOS, LEAK) are defined in mt-preparations, and the correspondi ...respiratory capacity [2-5]. Analysis of combined NS- versus single N- and S-pathway capacities yields information on pathway interactions and channelin

...d controls. In permeabilized fibroblasts we observed differences in CII/E, Rot/E, R/CII, CI/CII (p<0.05) between groups. These data highlight the dysfunct |pathways=N, S, NS, ROX

...exerted an inhibitory effect on succinate-pathway (S-pathway) and NADH and S (NS-) pathway, whereas 0.5 mM malate was apparently saturating for NADH (N- ...fect of 2 mM malate on succinate-linked respiration at 10 mM succinate (S, Rot). The inhibitory effect is less at 0.5 mM malate (400 mM stock for 0.5 mM e

...d controls. In permeabilized fibroblasts we observed differences in СII/E, Rot/E, R/CII, CI/CII (p<0,05) between groups. These data highlight the dysfunct ...of HRR in revealing the biochemical abnormalities of complex I in patient’s fibroblasts with LHON and Leigh-like syndrome. We also suggest HRR to be us

...were permeabilized by saponin [1]. Using SUIT protocol (G, M, P, D, c, S, Rot, Ama, As+Tm, Azd) standard respiratory states (LEAK, OXPHOS, and ROX), were |pathways=N, S, CIV, NS, ROX

|pathways=F, N, S, NS, ROX ...and GMS_''E'': glutamate+malate+succinate), and CII-substrate (S(Rot)_''E'': succinate+rotenone); ''N'': number of horses (2 or 3 runs

...cclimatization, with a 31% increased reliance on CI vs. CII respiration (S-Rot) and no significant effect on total ET capacity/mg of tissue (FCCP uncouple |pathways=N, S

|abbr=OXPHOS (GM+S+Rot+Omy) ...the [[NS-pathway control state| NS-]], [[Succinate pathway control state| S]] and [[NADH Electron transfer-pathway state| N-]] pathway control state.

...[[electron transfer pathway]] (ET pathway) in [[mitochondrial preparation]]s or living cells. Different conditions designated as ROX states (different c :::: 'KCN, H₂S and CO combine with some of the components of oxidase forming an inactive c

::::* P/M/S = [[PMS |Pyruvate&malate&succinate, PMS]], [[NS-pathway control state |NS-p ::::* S/Rot = [[Succinate pathway control state |Succinate&rotenone, SRot]], S-pathway

..., Flensted-Jensen M, Ingersen A, Morville T, Dela F, Wulff Helge J, Larsen S (2021) Reliability and variation in mitochondrial respiration in human adip ...n transfer, G: glutamate, M: malate, O: octanoyl carnitine, Rot: rotenone, S: succinate]]

# Israelson A, Arbel N, da Cruz S, Ilieva H, Yamanaka K, Shoshan-Barmatz V, Cleveland DW (2010) Misfolded mut # Magrì A, Reina S, De Pinto V (2018) VDAC1 as pharmacological target in cancer and neurodegen

...) cycle will, besides stimulating NADH production, also produce succinate (S) feeding CII. The extent of the contribution of CII to the overall respirat ...prehensive SUIT protocol using sequential titrations of PMG, ADP, S, FCCP, Rot and Ama (Fig.1). To determine the optimum concentration of M as well as opt

::::* [[SUIT-003 O2 ce D028]] for cell membrane permeability test CCP(S) ::::* ce4Rot: ROX (rotenone, Rot; residual oxygen consumption);

| A: Additivity between the N- and S-pathway in the Q-junction | 1S(Rot)

.... Best estimates of muscular OXPHOS capacity at 37 °C [pmol O₂∙s­^-1∙mg^-1 wet weight] with isolated mitochondria or ...d respiration (N-respiration; CI-linked) and succinate-linked respiration (S-respiration; CII-linked) are distinguished from combined NS-respiration (CI

...ria E, Casado M, Fernandez-Ortiz M, Acuna-Castroviejo D, Villena J, Grefte S, Keijer J, O'Brien KA, Sowton A, Murray AJ, Campbell MD, Marcinek DJ ...EE Tallinn Kaambre T, FR Montpellier Wrutniak-Cabello C, DE Nuthetal Klaus S, DE Duesseldorf Roden M, IT Verona Calabria E, ES Valencia Casado Pinna M,

...µL (5 x 10 nM), 5 x 1 µL (5 x 50 nM), 7 x 2 µL (7 x 100 nM); interval: 120 s ::::* [[TMRM]] was added 20-30 s before the addition of imt to the O2k chamber.

...ng 200 pmol∙^-11∙mg^-1 Ww [3], compared to 153±19 pmol∙s^-1∙mg^-1 Ww in competitive racing horses [4]. ...5 mM; glutamate, G 10 mM (PGM: CI); succinate, S 10 mM (CI&II); rotenone, Rot 0.5 µM (CII); residual oxygen consumption, ROX with malonate, 5 mM, and an

...ng 200 pmol∙s^-1∙mg^-1 Ww [3], compared to 153±19 pmol∙s^-1∙mg^-1 Ww in competitive racing horses [4]. ...5 mM; glutamate, G 10 mM (PGM: CI); succinate, S 10 mM (CI&II); rotenone, Rot 0.5 μM (CII); residual oxygen consumption, ROX with malonate, 5 mM, and an

...ng 200 pmol∙s^-1∙mg^-1 Ww [3], compared to 153±19 pmol∙s^-1∙mg^-1 Ww in competitive racing horses [4]. ...5 mM; glutamate, G 10 mM (PGM: CI); succinate, S 10 mM (CI&II); rotenone, Rot 0.5 μM (CII); residual oxygen consumption, ROX with malonate, 5 mM, and an

...linked ET capacity, CI&II_''E''; inhibition of CI with Rotenone (Rot) allows evaluation of CII-linked ET capacity (CII_E) and addition # Ferlay J, Steliarova-Foucher E, Lortet-Tieulent J, Rosso S, Coebergh JW, Comber H, et al (2013) Cancer incidence and mortality pattern

...at in the Yépez paper they did not titrate Ama. I believe ''Rox'' is after Rot titration. ...comparison of data from two different laboratories. {Jenny: Also, in Gargi's paper the Seahorse data and the O2k data are treated as separate but comple

::::* Interval 4: Rbl (Rot&Ama) - take the minimum {{Template:Correction FADH2 and S-pathway}}

...CI-CI<small>&</small>II-CII SUIT protocol was selected: GM +D +c +P +S +U +Rot +Ama (further details: [[MiPNet09.12 O2k-Titrations |»O2k-Titrations]]. [[File:Arctic-charr Koerbersee2014-IOC80(s).jpg |left|300px |Arctic charr]]

::::* [[Succinate]] (S) ::::* [[Rotenone]] (Rot)

...x slip contribute significantly to mitochondrial respiration? In: Schuster S, Rigoulet M, Ouhabi R, Mazat J-P (eds) Modern Trends in Biothermokinetics. :::# Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000b) Mitochondria in th

...x slip contribute significantly to mitochondrial respiration? In: Schuster S, Rigoulet M, Ouhabi R, Mazat J-P (eds) Modern Trends in Biothermokinetics. # [[Gnaiger 2000 Life in the Cold|Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000b) Mitochondria in th

...al-time_OXPHOS_analysis |Real-time OXPHOS analysis]]'''. — Richard Altmann’s bioblasts are the systematic unit of bioenergetics and chemiosmotic couplin ...the error of comparing NADH in the N-pathway with FADH₂ in the S-pathway ― together with fumarate, FADH₂ is a product but not a

...diabetes, neurodegenerative diseases (Alzheimer’s, Parkinson’s, Huntington’s), and several types of cancer. As a result of a sedentary life style and pr Image:Logo MitoFit s.jpg