Difference between revisions of "SUIT protocol library"
From Bioblast
Line 1: | Line 1: | ||
'''''This page is in preparation''''' (intern wiki: SUIT protocol library intern) | '''''This page is in preparation''''' (intern wiki: SUIT protocol library intern) | ||
<big><big>'''[[Substrate-uncoupler-inhibitor titration]] (SUIT) protocols | <big><big>'''[[Substrate-uncoupler-inhibitor titration]] (SUIT) protocols</big> | ||
Classes of SUIT protocols with coupling and substrate control'''</big> | Classes of SUIT protocols with coupling and substrate control'''</big> | ||
Β | |||
Β | |||
__TOC__ | |||
== Structure == | |||
# Select a [[#Pathway control states |pathway control state]] | |||
# Define substrates for each pathway state | |||
== Definition of states == | == Definition of states == | ||
Line 23: | Line 29: | ||
== | == Pathway control states == | ||
=== Single | === Single pathway control states === | ||
# CI | # CI | ||
Line 34: | Line 40: | ||
# CGPDH | # CGPDH | ||
=== Multiple | === Multiple pathway control states === | ||
'''A. CI-CII-CIV''' | '''A. CI-CII-CIV''' | ||
Line 59: | Line 65: | ||
'''D. FAO-CGPDH-CI-CII-CIV''' | '''D. FAO-CGPDH-CI-CII-CIV''' | ||
# FAO + CGPDH + CI + | # FAO + CGPDH + CI + ... | ||
# FAO | # CGPDH + FAO + CI + ... | ||
# FAO + CGPDH + | # CI + FAO + CGPDH + ... | ||
# | # CI + CGPDH + FAO + ... | ||
# ... | # ... | ||
Revision as of 03:30, 19 August 2015
This page is in preparation (intern wiki: SUIT protocol library intern)
Substrate-uncoupler-inhibitor titration (SUIT) protocols
Classes of SUIT protocols with coupling and substrate control
Structure
- Select a pathway control state
- Define substrates for each pathway state
Definition of states
Define substrates
- CI: PM, GM, PGM, M, ..
- CII: S(Rot), S, SM, ..
- CI&II: PS, GS, PMS, GMS, PGMS, ..
- CIV: AsTM
- FAO: Oct, Pal, ...
- CGPDH:
- c
Define coupling states
- L, P, E
Define residual oxygen consumption
- ROX: Rot&Ama, Rot&Mna&Ama, Cn, Az, ..
Pathway control states
Single pathway control states
- CI
- CII
- CI&CII
- CIV
- FAO
- CGPDH
Multiple pathway control states
A. CI-CII-CIV
- CI + CII
- CI + CII + CIV
- CI + CI&CII + CII
- CI + CI&CII + CII + CIV
B. FAO-CI-CII-CIV
- FAO + CI + CII
- FAO + CI + CII + CIV
- FAO + CI + CI&CII + CII
- FAO + CI + CI&CII + CII + CIV
C. CGPDH-CI-CII-CIV
- CGPDH + CI
- CGPDH + CI + CII
- CGPDH + CI + CII + CIV
- CGPDH + CI + CI&CII + CII
- CGPDH + CI + CI&CII + CII + CIV
- CI + CGPDH + ...
- CI + CI&CII + CGPDH + ...
- CI&CII + CGPDH + ...
D. FAO-CGPDH-CI-CII-CIV
- FAO + CGPDH + CI + ...
- CGPDH + FAO + CI + ...
- CI + FAO + CGPDH + ...
- CI + CGPDH + FAO + ...
- ...
Control patterns
Linear
- Linear coupling control: at a single substrate state (classical State 3 -> State 4, or P -> LT).
- Linear substrate control: at a single coupling state (unusual).
- Monodirectional linear coupling control: LN -> PD -> EF
- Bidirectional linear coupling control: LN -> PD -> LOmy -> EF
Orthogonal
- Orthogonal coupling -> substrate control (mono- or bidirectional)
- Orthogonal substrate -> coupling control (mono- or bidirectional)
Diametral
- Diametral coupling -> substrate ->> control (mono- or bidirectional)
- Diametral substrate -> coupling ->> control (mono- or bidirectional)