Difference between revisions of "SUIT-007"
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== Strengths and limitations == | == Strengths and limitations == | ||
:::* | :::* Protocol focused on analysis of glutamate dehydrogenase anaplerotic pathway/glutaminolysis. | ||
:::+ The use of glutamate alone allows to analyse the glutamate anaplerotic pathway control state. | :::+ The use of glutamate alone allows to analyse the glutamate dehydrogenase anaplerotic pathway control state. | ||
:::+ Short duration of the experiment. | :::+ Short duration of the experiment. | ||
:::+ Glutamate is easier to prepare compared to pyruvate. | :::+ Glutamate is easier to prepare compared to pyruvate. |
Revision as of 16:38, 22 January 2019
Description
Abbreviation: N(GM)
Reference: A: »Versions
- SUIT-category: N(GM)
- SUIT protocol pattern: diametral 1G;2D;2c;3M;4U;5Ama
DLP applications
- SUIT-007 O2 pce D030 for permeabilized cells
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1G | GL | (N) | CI | 1G
|
2D | GP | (N) | CI | 1G;2D
|
2c | GcP | (N) | CI | 1G;2D;2c
|
3M | GMP | N | CI | 1G;2D;2c;3M
|
4U | GME | N | CI | 1G;2D;2c;3M;4U
|
5Ama | ROX | 1G;2D;2c;3M;4U;5Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- Protocol focused on analysis of glutamate dehydrogenase anaplerotic pathway/glutaminolysis.
- + The use of glutamate alone allows to analyse the glutamate dehydrogenase anaplerotic pathway control state.
- + Short duration of the experiment.
- + Glutamate is easier to prepare compared to pyruvate.
- + CIV activity can be measured after this protocol.
- - Analysis of N-OXPHOS capacity (instead of NS-OXPHOS capacity) does not provide the best estimate of maximum mitochondrial respiratory capacity.
- - This protocol does not address questions regarding the convergence of pathways at the Q-junction.
- - Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler.
Compare SUIT protocols
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry