- high-resolution terminology - matching measurements at high-resolution
NADH electron transfer-pathway state
Description
[[Description::
The NADH pathway control state (N) is obtained by addition of NADH-linked substrates (CI-linked), feeding electrons into the N-junction catalyzed by various mt-dehydrogenases. N-supported flux is induced in mt-preparations by addition of NADH-generating substrate combinations of pyruvate (P), glutamate (G), malate (M), oxaloacetate (Oa), oxoglutarate (Og), citrate, hydroxybutyrate. These N-junction substrates are (indirectly) linked to Complex I by the corresponding dehydrogenase-catalyzed reactions reducing NAD+ to NADH+H+. The most commonly applied N-junction substrate combinations are: PM, GM, PGM. The malate anaplerotic pathway control state (M alone) is a special case related to malic enzyme (mtME). The glutamate anaplerotic pathway control state (aN; G alone) supports respiration through glutamate dehydrogenase (mtGDH). In mt-preparations, succinate dehydrogenase (SDH; CII) is largely substrate-limited in N-linked respiration, due to metabolite depletion into the incubation medium. The residual involvement of S-linked respiration in the N-pathway control state can be further suppressed by the CII-inhibitor malonic acid). In the N-pathway control state ETS pathway level 4 is active.]]
Abbreviation: Has abbr::N
Reference: [[Info::Pathway control state, Gnaiger 2014 MitoPathways]]
MitoPedia concepts:
MiP concept,
Respiratory state,
SUIT state,
Recommended
MitoPedia methods:
Respirometry
MitoPedia topics:
MitoPedia topic::EAGLE
Communicated by Gnaiger E 2016-03-20, edited 2016-11-29.
From CI to N
Publications: N-pathway control state
Sort in ascending/descending order by a click on one of the small symbols in squares below. Default sorting: chronological. Empty fields appear first in ascending order.
{{#ask: Pathways::N |?Was published in year=Year |?Has title=Reference |?Coupling states=Coupling |?Pathways=Pathway |?Preparation=Preparation |?Mammal and model=Organism |?Tissue and cell=Tissue;cell |format=broadtable |limit=5000 |offset=0 |sort=Was published in year |order=descending }}
Abstracts: N-pathway control state
Sort in ascending/descending order by a click on one of the small symbols in squares below.
{{#ask: Pathways::N |?Was submitted in year=Year |?Has title=Reference |?Coupling states=Coupling |?Pathways=Pathway |?Preparation=Preparation |?Mammal and model=Organism |?Tissue and cell=Tissue;cell |format=broadtable |limit=5000 |offset=0 |sort=Was submitted in year |order=descending }}
