Difference between revisions of "MiR05Cr"
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{{MitoPedia | {{MitoPedia | ||
|abbr=MiR05Cr | |abbr=MiR05Cr | ||
|description=Mitochondrial respiration medium, '''MiR05Cr''', developed for oxygraph incubations of mitochondrial preparations - ''[[permeabilized muscle | |description=Mitochondrial respiration medium, '''MiR05Cr''', developed for oxygraph incubations of mitochondrial preparations - ''[[permeabilized muscle fibers]]''. | ||
MiR05Cr = [[MiR05]] + 20 mM [[Creatine|creatine]]. | MiR05Cr = [[MiR05]] + 20 mM [[Creatine|creatine]]. | ||
|info=[[MiPNet14.13]] | |info=[[MiPNet14.13]] | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia concepts}} | ||
{{MitoPedia methods}} | |||
}} | {{MitoPedia O2k and high-resolution respirometry}} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Medium | |mitopedia topic=Medium | ||
}} | }} | ||
The '''oxygen solubility factor''' of MiR05Cr is the same as for [[MiR05]] = '''0.92'''. | * For preparation of MiR05Cr and application in HRR, see [[MiPNet14.13 Medium-MiR06]] | ||
* The '''oxygen solubility factor''' of MiR05Cr is the same as for [[MiR05]] = '''0.92'''. | |||
'''Rationale:''' as for [[MiR05]], but less ADP is needed in permeabilized muscle | '''Rationale:''' as for [[MiR05]], but less ADP is needed in permeabilized muscle fibers. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect. | ||
Latest revision as of 18:46, 15 May 2019
- high-resolution terminology - matching measurements at high-resolution
MiR05Cr
Description
Mitochondrial respiration medium, MiR05Cr, developed for oxygraph incubations of mitochondrial preparations - permeabilized muscle fibers.
MiR05Cr = MiR05 + 20 mM creatine.
Abbreviation: MiR05Cr
Reference: MiPNet14.13
MitoPedia topics: Media for respirometry
- For preparation of MiR05Cr and application in HRR, see MiPNet14.13 Medium-MiR06
- The oxygen solubility factor of MiR05Cr is the same as for MiR05 = 0.92.
Rationale: as for MiR05, but less ADP is needed in permeabilized muscle fibers. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect.
