Luevano-Martinez 2015 Biochim Biophys Acta: Difference between revisions
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{{Publication | {{Publication | ||
|title=LuΓ©vano-MartΓnez LA, Forni MF, Dos Santos VT, Souza-Pinto NC, Kowaltowski AJ (2015) Cardiolipin is a key determinant for mtDNA stability and segregation during mitochondrial stress. Biochim Biophys Acta 1847:587-98. | |title=LuΓ©vano-MartΓnez LA, Forni MF, Dos Santos VT, Souza-Pinto NC, Kowaltowski AJ (2015) Cardiolipin is a key determinant for mtDNA stability and segregation during mitochondrial stress. Biochim Biophys Acta 1847:587-98. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/25843549 PMID:25843549] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/25843549 PMID:25843549 Open Access] | ||
|authors=Luevano-Martinez LA, Forni MF, Dos Santos VT, Souza-Pinto NC, Kowaltowski AJ | |authors=Luevano-Martinez LA, Forni MF, Dos Santos VT, Souza-Pinto NC, Kowaltowski AJ | ||
|year=2015 | |year=2015 | ||
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{{Labeling | {{Labeling | ||
|area=Respiration, mt-Membrane, mtDNA;mt-genetics | |area=Respiration, mt-Membrane, mtDNA;mt-genetics | ||
|organism=Saccharomyces cerevisiae | |injuries=Temperature | ||
|organism=Saccharomyces cerevisiae, Fungi | |||
|preparations=Intact cells | |preparations=Intact cells | ||
|enzymes=Complex III, Complex IV;cytochrome c oxidase | |enzymes=Complex III, Complex IV;cytochrome c oxidase | ||
|couplingstates=ROUTINE, ET | |||
|couplingstates=ROUTINE, | |||
|pathways=ROX | |pathways=ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 12:39, 2 August 2023
| LuΓ©vano-MartΓnez LA, Forni MF, Dos Santos VT, Souza-Pinto NC, Kowaltowski AJ (2015) Cardiolipin is a key determinant for mtDNA stability and segregation during mitochondrial stress. Biochim Biophys Acta 1847:587-98. |
Luevano-Martinez LA, Forni MF, Dos Santos VT, Souza-Pinto NC, Kowaltowski AJ (2015) Biochim Biophys Acta
Abstract: Mitochondria play a key role in adaptation during stressing situations. Cardiolipin, the main anionic phospholipid in mitochondrial membranes, is expected to be a determinant in this adaptive mechanism since it modulates the activity of most membrane proteins. Here, we used Saccharomyces cerevisiae subjected to conditions that affect mitochondrial metabolism as a model to determine the possible role of cardiolipin in stress adaptation. Interestingly, we found that thermal stress promotes a 30% increase in the cardiolipin content and modifies the physical state of mitochondrial membranes. These changes have effects on mtDNA stability, adapting cells to thermal stress. Conversely, this effect is cardiolipin-dependent since a cardiolipin synthase-null mutant strain is unable to adapt to thermal stress as observed by a 60% increase of cells lacking mtDNA (Ο(0)). Interestingly, we found that the loss of cardiolipin specifically affects the segregation of mtDNA to daughter cells, leading to a respiratory deficient phenotype after replication. We also provide evidence that mtDNA physically interacts with cardiolipin both in S. cerevisiae and in mammalian mitochondria. Overall, our results demonstrate that the mitochondrial lipid cardiolipin is a key determinant in the maintenance of mtDNA stability and segregation. β’ Keywords: Phospholipid, Mitochondrion, Mitochondrial DNA, Membrane plasticity
β’ O2k-Network Lab: BR Sao Paulo Kowaltowski AJ
Labels: MiParea: Respiration, mt-Membrane, mtDNA;mt-genetics
Stress:Temperature Organism: Saccharomyces cerevisiae, Fungi
Preparation: Intact cells Enzyme: Complex III, Complex IV;cytochrome c oxidase
Coupling state: ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k
