Komlodi Timea

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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

Komlodi Timea

MitoPedia topics: MitoPedia topic::EAGLE

COST: Has COST::Member COST WG1: Has COST WG1::WG1

COST WG4: Has COST WG4::WG4

COST ECI: Has COST ECI::ECI

Name	Has lastname::Komlòdi Has firstname::Timea, Has title::Dr.
Institution	[[Has institution::::::::::::::::::: Oroboros Instrument Mitochondria and cell research Research assistant K-Regio MitoFit H2O2 experiments Timea Komlòdi joined Oroboros in February 2017.]]
Address	Has address::Schöpfstraße 18, Has area code::
City	In city::Innsbruck
State/Province	In state::
Country	In country::Austria
Email	Has mailaddress::[email protected]
Weblink	Weblink::
O2k-Network Lab	{{#ask:has member::Komlodi Timea}}

Labels: Field of research: field of research::Basic

Publications

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Abstracts

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MitoEAGLE Short-Term Scientific Mission

- - - STSM Grant Period 1

Motivation letter

I am writing this letter to apply for the Short-Term Scientific Mission (STSM) covered by MitoEAGLE–COST Action CA15203 (1st Call for STSM Applications).

My name is Tímea Komlódi. I studied at Semmelweis University at the Faculty of Pharmacy. After graduation, in 2013, I started working as a PhD student at the Semmelweis University in the Department of Medical Biochemistry, Hungary.

My main topic of interest is energy metabolism and mitochondrial homeostasis under physiological and pathological conditions. Our group focuses mainly on neurodegenerative diseases like Alzheimer’s and Parkinson’s disease, which are attributed to mitochondrial dysfunction. I have experience in measurement of mitochondrial respiration, ATP synthesis, membrane potential, reactive oxygen species production and intra- and extramitochondrial pH detection.

In the future I am planning to deepen my knowledge about mitochondria and to expand it towards cell lines. The STSM grant would give the opportunity to deal with mitochondrial physiology and cell lines at the Oroboros Instruments in Innsbruck. The focus of the STSM project is the standardization of protocols in mitochondrial respirometry which could contribute to the scientific objective of MitoEAGLE. During the 8-day STSM mission I could acquire new measurement protocols which could be applied in future projects. These are the planned program of the STSM mission: 1.) detection of reactive oxygen species production of permeabilized cells using fluorescent probes; 2.) determination of mitochondrial membrane potential with safranin, TMRM and TPP+; 3.) assessment of mitochondrial P/O ratio by measuring ATP levels fluorometrically.

The STSM training would provide a platform for an international discussion and standardization of protocols, data and measurement techniques. Furthermore, the STSM project could be a basis of a flourishing cooperation with the Oroboros laboratory in Innsbruck in the near future.

I would like to participate in the MitoEAGLE projects because it provides the opportunity to

take part in data establishment and comparison of data between different research groups.

Moreover, Oroboros laboratory would provide a platform with its instrumental background,with its protocols and with its international connections to the objectives and aim of MitoEAGLE.

STSM request: download pdf

MitoEAGLE

- - - Benefits for COST and for the COST Action MitoEAGLE

During my PhD I worked with brain, heart and liver mitochondria isolated from guinea pig and in this summer I started to deal with cultured cell lines

Participated at

Visiting scientist in the Oroboros MitoFit Laboratory

Timea Komlòdi : Visiting scientist at the Oroboros MitoFit Laboratory

November 21 to 28 2016